LOCUS       sequence-49033-e        1227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1227)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CUL5
FEATURES             Location/Qualifiers
     source          1..1227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1156..1176
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1177..1194
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1195..1218
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggcgacgt ctaatctgtt aaagaataaa ggttctctcc agtttgaaga caagtgggac
       61 ttcatgcatc caattgtttt gaagctttta cgccaggaat ctgtaacaaa acagcagtgg
      121 tttgatctat tttcggatgt acatgctgtc tgtctctggg atgataaagg ctcatcaaaa
      181 attcatcagg ctttaaaaga agatattctt gagtttatta agcaagcaca ggctcgtgta
      241 ctgagccatc aagatgacac agctttgctg aaggcatata ttgttgaatg gcggaaattc
      301 ttcacacagt gtgatatttt accaaaacct ttttgtcaat tagaggtgac tctattgggt
      361 aaacaaagca gcaataaaaa atcaaatatg gaagacagta ttgttcgaaa gctcatgctt
      421 gatacgtgga atgagtcgat tttttcaaat ataaagaaca gactccagga cagtgcaatg
      481 aagctggtgc atgctgagag attaggggaa gcttttgatt cccagctggt catcggggtg
      541 cgagagtcct atgttaatct ttgctccaac cccgaggaca agcttcagat ctatagggat
      601 aattttgaga aggcatactt ggactcaaca gagaggtttt atagaacaca ggcaccctca
      661 tatttacagc aaaatggtgt gcagaattac atgaaatatg cagatgctaa attaaaagaa
      721 gaagaaaaaa gagcactccg atacttagaa acacgacgag agtgtaactc tgtggaagca
      781 ctcatggaat gctgtgtaaa tgcgctggtg acctccttta aagagactat tttagcagaa
      841 tgccaaggca tgatcaagcg aaatgaaact gaaaagttac atttgatgtt ttccttgatg
      901 gacaaagttc ctaatgggat agagccgatg ttgaaggact tggaggagca tattataagt
      961 gcgggcctag cagacatggt ggccgcagct gaaaccatca ctactgactc tgagaagtat
     1021 cgggagcaat tagacacact gtttaataga ttcagtaaac tggtcaaaga agcttttcag
     1081 gatgatcctc gtttccttac tgcaagagat aaggcatata aagcagttgt taatgatgct
     1141 actatattta aagcagagaa cctctacttc caatcgcacc atcatcacca ccatgattac
     1201 aaggatgacg acgataagtg aggatcc
//
