LOCUS       sequence-49039-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SPSB1
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc aggagctcca gggtctggat tactgcaagc ccacccggct ggatctgcta
      121 ctggacatgc cccctgtgtc ctatgatgtc cagctgctgc attcatggaa caacaacgac
      181 cgatcgctca atgtctttgt gaaggaggac gacaagctca tctttcaccg gcatccggtg
      241 gcccagagca cggacgctat caggggcaaa gtcgggtata cccgtgggct gcacgtgtgg
      301 cagatcacgt gggccatgag acagcggggc acacacgccg tggtgggggt ggcgacggca
      361 gacgcccccc tgcactctgt cgggtacaca accctcgtgg ggaataacca cgagtcctgg
      421 ggctgggact tggggcgcaa ccggctctac cacgatggca agaaccagcc aagcaaaaca
      481 tacccagcct ttctggaacc agatgagaca ttcattgtcc ctgactcctt cctggtagcc
      541 ctggacatgg acgacgggac tctgagcttc attgtggatg gacagtacat gggagtggct
      601 tttcggggac tcaagggcaa aaaactgtat cctgtagtga gtgccgtctg gggccactgt
      661 gagatccgaa tgcgctactt gaacggactc gatcccgagt gacagtaaag gtggata
//
