LOCUS       sequence-49068-e        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GAPDHS
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1021..1041
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1042..1059
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1060..1083
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggtgtctg tggcccggga gctgactgtg ggcatcaatg gatttggacg catcggtcgc
       61 ctggtcctgc gcgcctgcat ggagaagggt gttaaggtgg tggctgtgaa tgatccattc
      121 attgacccgg aatacatggt gtacatgttt aagtatgact ccacccacgg ccgatacaag
      181 ggaagtgtgg aattcaggaa tggacaactg gtcgtggaca accatgagat ctctgtctac
      241 cagtgcaaag agcccaaaca gatcccctgg agggctgtcg ggagccccta cgtggtggag
      301 tccacaggcg tgtacctctc catacaggca gcttcggacc acatctctgc aggtgctcaa
      361 cgtgtggtca tctccgcgcc ctcaccggat gcaccaatgt tcgtcatggg tgtcaatgaa
      421 aatgactata accctggctc catgaacatt gtgagcaacg cgtcctgcac caccaactgt
      481 ttggctcccc tcgccaaagt catccacgag cgatttggga tcgtggaagg gttgatgacc
      541 acagtccatt cctacacggc cacccagaag acagtggacg ggccatcaag gaaggcctgg
      601 cgagatgggc ggggtgccca ccagaacatc atcccagcct ccactggggc tgcgaaagct
      661 gtgaccaaag tcatcccaga gctcaaaggg aagctgacag ggatggcgtt ccgggtacca
      721 accccggatg tgtctgtcgt ggacctgacc tgccgcctcg cccagcctgc cccctactca
      781 gccatcaagg aggctgtaaa agcagcagcc aaggggccca tggctggcat ccttgcctac
      841 accgaggatg aggtcgtctc tacggacttc ctcggtgata cccactcgtc catcttcgat
      901 gctaaggccg gcattgcgct caatgacaat ttcgtgaagc tcatttcatg gtacgacaac
      961 gaatatggct acagtcaccg ggtggtcgac ctcctccgct acatgttcag ccgagacgca
     1021 gagaacctct acttccaatc gcaccatcat caccaccatg attacaagga tgacgacgat
     1081 aagtgaggat cc
//
