LOCUS       sequence-490837-        9970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ADAR1-p150 from the CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Kolber NS, K. Kaseniit E, Lanz TV, Robinson WH, Gao XJ
  TITLE     Sequence-Independent RNA Sensing in Living Mammalian Cells
  JOURNAL   bioRxiv 2025.09.18.677213
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.18.677213"
COMMENT     Sequence Label: EK0574 CAG-ADAR1-p150
FEATURES             Location/Qualifiers
     source          1..9970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(170..961,962..1030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     793..812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1031..1135)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1210..1229)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1396..1775
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1778..2052
                     /label=chicken beta-actin promoter
     intron          2053..3061
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2985..3007
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      3142..3151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3163..3228
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6925..6942
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6964..6993
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(7049..7066)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7055..7279
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7451..7470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7602..7649
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7657..8679
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7657..8679
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8809..8930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8846..8865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8900..8919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8979..8995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8979..8995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8992..9014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9003..9019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9027..9033,9034..9051,9052..9057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9072..9093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9210..9227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9381..9969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9461..9480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1141 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     1201 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     1261 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     1321 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgtacggg
     1381 ccagatatac gcgttgacat tgattattga ctagttatta ttagtaatca attacggggt
     1441 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     1501 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     1561 taacgccaat agggactttc cattgacgtc aatgggtgga ctatttacgg taaactgccc
     1621 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     1681 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     1741 agtacatcta cgtattagtc atcgctatta ccatgggtcg aggtgagccc cacgttctgc
     1801 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttat
     1861 ttattttgtg cagcgatggg ggcggggggg ggggggggcg cgccaggcgg ggcggggcgg
     1921 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     1981 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     2041 gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg
     2101 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     2161 ccttctcctc cgggctgtaa ttagcgcttg gtttattgac ggctcgtttc ttttctgtgg
     2221 ctgcgtgaaa gccttatagg gctccgggag ggccctttgt gcggggggga gcggctcggg
     2281 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc
     2341 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg
     2401 cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg
     2461 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc
     2521 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg
     2581 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     2641 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag
     2701 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     2761 gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc
     2821 cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     2881 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct
     2941 gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     3001 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     3061 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag cacgtgagga
     3121 ccgggtttaa acttaagctt cgccaccatg gggtcccggg ctgactacaa agaccatgac
     3181 ggtgattata aagatcatga catcgactac aaggatgacg atgacaaggg gcgcgcccta
     3241 gtaaatccgc ggcaggggta ttccctcagc ggatactaca cccatccatt tcaaggctat
     3301 gagcacagac agctcagata ccagcagcct gggccaggat cttcccccag tagtttcctg
     3361 cttaagcaaa tagaatttct caaggggcag ctcccagaag caccggtgat tggaaagcag
     3421 acaccgtcac tgccaccttc cctcccagga ctccggccaa ggtttccagt actacttgcc
     3481 tccagtacca gaggcaggca agtggacatc aggggtgtcc ccaggggcgt gcatctcgga
     3541 agtcaggggc tccagagagg gttccagcat ccttcaccac gtggcaggag tctgccacag
     3601 agaggtgttg attgcctttc ctcacatttc caggaactga gtatctacca agatcaggaa
     3661 caaaggatct taaagttcct ggaagagctt ggggaaggga aggccaccac agcacatgat
     3721 ctgtctggga aacttgggac tccgaagaaa gaaatcaatc gagttttata ctccctggca
     3781 aagaagggca agctacagaa agaggcagga acaccccctt tgtggaaaat cgcggtctcc
     3841 actcaggctt ggaaccagca cagcggagtg gtaagaccag acggtcatag ccaaggagcc
     3901 ccaaactcag acccgagttt ggaaccggaa gacagaaact ccacatctgt ctcagaagat
     3961 cttcttgagc cttttattgc agtctcagct caggcttgga accagcacag cggagtggta
     4021 agaccagaca gtcatagcca aggatcccca aactcagacc caggtttgga acctgaagac
     4081 agcaactcca catctgcctt ggaagatcct cttgagtttt tagacatggc cgagatcaag
     4141 gagaaaatct gcgactatct cttcaatgtg tctgactcct ctgccctgaa tttggctaaa
     4201 aatattggcc ttaccaaggc ccgagatata aatgctgtgc taattgacat ggaaaggcag
     4261 ggggatgtct atagacaagg gacaacccct cccatatggc atttgacaga caagaagcga
     4321 gagaggatgc aaatcaagag aaatacgaac agtgttcctg aaaccgctcc agctgcaatc
     4381 cctgagacca gaagaaacgc agagttcctc acctgtaata tacccacatc aaatgcctca
     4441 aataacatgg taaccacaga aaaagtggag aatgggcagg aacctgtcat aaagttagaa
     4501 aacaggcaag aggccagacc agaaccagca agactgaaac cacctgttca ttacaatggc
     4561 ccctcaaaag cagggtatgt tgactttgaa aatggccagt gggccacaga tgacatccca
     4621 gatgacttga atagtatccg cgcagcacca ggtgagtttc gagccatcat ggagatgccc
     4681 tccttctaca gtcatggctt gccacggtgt tcaccctaca agaaactgac agagtgccag
     4741 ctgaagaacc ccatcagcgg gctgttagaa tatgcccagt tcgctagtca aacctgtgag
     4801 ttcaacatga tagagcagag tggaccaccc catgaacctc gatttaaatt ccaggttgtc
     4861 atcaatggcc gagagtttcc cccagctgaa gctggaagca agaaagtggc caagcaggat
     4921 gcagctatga aagccatgac aattctgcta gaggaagcca aagccaagga cagtggaaaa
     4981 tcagaagaat catcccacta ttccacagag aaagaatcag agaagactgc agagtcccag
     5041 acccccaccc cttcagccac atccttcttt tctgggaaga gccccgtcac cacactgctt
     5101 gagtgtatgc acaaattggg gaactcctgc gaattccgtc tcctgtccaa agaaggccct
     5161 gcccatgaac ccaagttcca atactgtgtt gcagtgggag cccaaacttt ccccagtgtg
     5221 agtgctccca gcaagaaagt ggcaaagcag atggccgcag aggaagccat gaaggccctg
     5281 catggggagg cgaccaactc catggcttct gataaccagc ctgaaggtat gatctcagag
     5341 tcacttgata acttggaatc catgatgccc aacaaggtca ggaagattgg cgagctcgtg
     5401 agatacctga acaccaaccc tgtgggtggc cttttggagt acgcccgctc ccatggcttt
     5461 gctgctgaat tcaagttggt cgaccagtcc ggacctcctc acgagcccaa gttcgtttac
     5521 caagcaaaag ttgggggtcg ctggttccca gccgtctgcg cacacagcaa gaagcaaggc
     5581 aagcaggaag cagcagatgc ggctctccgt gtcttgattg gggagaacga gaaggcagaa
     5641 cgcatgggtt tcacagaggt aaccccagtg acaggggcca gtctcagaag aactatgctc
     5701 ctcctctcaa ggtccccaga agcacagcca aagacactcc ctctcactgg cagcaccttc
     5761 catgaccaga tagccatgct gagccaccgg tgcttcaaca ctctgactaa cagcttccag
     5821 ccctccttgc tcggccgcaa gattctggcc gccatcatta tgaaaaaaga ctctgaggac
     5881 atgggtgtcg tcgtcagctt gggaacaggg aatcgctgtg taaaaggaga ttctctcagc
     5941 ctaaaaggag aaactgtcaa tgactgccat gcagaaataa tctcccggag aggcttcatc
     6001 aggtttctct acagtgagtt aatgaaatac aactcccaga ctgcgaagga tagtatattt
     6061 gaacctgcta agggaggaga aaagctccaa ataaaaaaga ctgtgtcatt ccatctgtat
     6121 atcagcactg ctccgtgtgg agatggcgcc ctctttgaca agtcctgcag cgaccgtgct
     6181 atggaaagca cagaatcccg ccactaccct gtcttcgaga atcccaaaca aggaaagctc
     6241 cgcaccaagg tggagaacgg agaaggcaca atccctgtgg aatccagtga cattgtgcct
     6301 acgtgggatg gcattcggct cggggagaga ctccgtacca tgtcctgtag tgacaaaatc
     6361 ctacgctgga acgtgctggg cctgcaaggg gcactgttga cccacttcct gcagcccatt
     6421 tatctcaaat ctgtcacatt gggttacctt ttcagccaag ggcatctgac ccgtgctatt
     6481 tgctgtcgtg tgacaagaga tgggagtgca tttgaggatg gactacgaca tccctttatt
     6541 gtcaaccacc ccaaggttgg cagagtcagc atatatgatt ccaaaaggca atccgggaag
     6601 actaaggaga caagcgtcaa ctggtgtctg gctgatggct atgacctgga gatcctggac
     6661 ggtaccagag gcactgtgga tgggccacgg aatgaattgt cccgggtctc caaaaagaac
     6721 atttttcttc tatttaagaa gctctgctcc ttccgttacc gcagggatct actgagactc
     6781 tcctatggtg aggccaagaa agctgcccgt gactacgaga cggccaagaa ctacttcaaa
     6841 aaaggcctga aggatatggg ctatgggaac tggattagca aaccccagga ggaaaagaac
     6901 ttttatctct gcccagtact agtccaccat caccatcacc attaggccgc tcgaggaggg
     6961 cccgaacaaa aactcatctc agaagaggat ctgaatagcg ccgtcgacca tcatcataat
     7021 catcattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     7081 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7141 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7201 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     7261 gatgcggtgg gctctatgga gatctatcga tgttctgggg atgcggtggg ctctatggct
     7321 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     7381 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7441 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7501 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7561 gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt
     7621 tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg
     7681 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     7741 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     7801 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     7861 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     7921 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     7981 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     8041 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     8101 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     8161 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     8221 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     8281 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     8341 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     8401 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     8461 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     8521 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     8581 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     8641 aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat
     8701 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     8761 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     8821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     8941 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     9001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9961 ggatctcaag
//
