LOCUS       sequence-490851-        1239 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Blue Fluorescent Protein expressing plasmid with Puromycin selection
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1239)
  TITLE     Mx potein restrict viral replication in salmonid cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-Pur-BFP
FEATURES             Location/Qualifiers
     source          1..1239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..640)
                     /codon_start=1
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(696..1025)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      710..845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(764..783)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1010..1030
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 taggggcggt ggatcgaatc tcgtagcacg tgggcgcgcc ttaggcgcca ggttttcgtg
       61 tcatacacca ggtcctaggt ccctcgggca cctcgacatc ggcggtcacg gtgaagccca
      121 ggcgctcgta gaatggaagg tttcttggtg cgcttgtttc gagaaaggcg ggcacaccgg
      181 cgcgttcggc ggcctctacg ccgggcaaca cgacggcgct gcccaagccc ttgccctggt
      241 ggtcggggct cactcctaca gtggccagaa accaggcagg ctccttggga cgatgggggg
      301 caagcaggcc ctccatttgc tgctgggcgg ccaggcggct gccgctcagc tcggccattc
      361 gggggccgat ctcggcgaac acggcgccgg cctcaacgct ctcgggggta gtccacacgg
      421 ccacggccgc gccgtcgtcg gctacccaca ccttgccgat gtccagcccg acgcgggtca
      481 ggaacagctc ctgcaactct gtcacacgct cgatgtgccg gtcggggtcc acggtgtgcc
      541 gggtagcggg gtagtcggcg aaagcggcgg ccagagtgcg gacggcgcgg ggcacatcat
      601 cgcgagtggc caggcgcacg gtaggcttgt actcggtcat cctcaggcac gtgctgatca
      661 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
      721 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
      781 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
      841 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
      901 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
      961 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     1021 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1081 aataggccga aatcggcaaa atcccttata aatcaaaaaa tagaccgaga tagggttgag
     1141 tgttgttcca gtttggaaca agagtcacta ttaaagaacg tggactccac gtcaagggcg
     1201 aaaaaccgtc tatcagggcg atggccacta cgtgaacat
//
