LOCUS       sequence-490888-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for ARIH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     Schulman lab plasmids 2025
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GST-tag ARIH2 binding E2 variant L3A2-2
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             508..552
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(728..750)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 catcctccaa aatcggatga aaacctgtat tttcagggat ccatgaaagc ggccagcagg
       61 aggctgatga aggagcttga agaaatccgc aaagcaggga tgaaaaactt ccgtaacatc
      121 caggttgatg aagctaattt attgacttgg caagggctta ttgttcctga caaccctcca
      181 tatgataagg gagccttcag aatcgaaatc aactttccag cagagtaccc attcaaacca
      241 ccgaagatca catttaaaac aaagatctat cacccaaaca tcgacgaaaa ggggcaggtc
      301 tgtctgccag taattagtgc cgaaaactgg aagccagcaa ccaaaaccga ccaagtaatc
      361 cagtccctca tagcactggt gaatgacccc cagcctgagc acccgcttcg ggctgaccta
      421 gctgaagaat actctaagga ccgtaaaaaa ttcgcaaaga atgctgaaga gtttacaaag
      481 aaatatgggg aaaagcgacc tgtggacggt cttaacgaca tcttcgaggc tcaaaagatt
      541 gaatggcatg aataagcggc cgcatcgggc tgacctagct gaagaatact ctaaggaccg
      601 taaaaaattc gcaaagaatg ctgaagagtt tacaaagaaa tatggggaaa agcgacctgt
      661 ggactaagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg
      721 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      781 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
      841 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg
      901 tg
//
