LOCUS       sequence-490894-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for ANKIB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  TITLE     Schulman lab plasmids 2025
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GST-tag ANKIB1 binding E2 variant L3AN-1
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(523..545)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(690..708)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        776..880
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcggccagtc cgaggctgat ttgggagctt gaagaaatcc gcaaagcagg gatgaaaaac
       61 ttccgtaaca tccaggttga tgaagctaat ttattgactt ggcaagggct tattgttcct
      121 gacaaccctc catatgataa gggagccttc agaatcgaaa tcaactttcc agcagagtac
      181 ccattcaaac caccgaagat cacatttaaa acaaagatct atcacccaaa catcgacgaa
      241 aaggggcagg tctgtctgcc agtaattagt gccgaaaact ggaagccagc aaccaaaacc
      301 gaccaagtaa tccagtccct catagcactg gtgaatgacc cccagcctga gcacccgctt
      361 cgggctgacc tagctgaaga atactctaag gaccgtaaaa aattcgcaaa gaatgctgaa
      421 gagtttacaa agaaatatgg ggaaaagcga cctgtggact aagcggycgc atcgtgactg
      481 actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
      541 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
      601 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
      661 cggagtgtat aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      721 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      781 aacccctatt tgwttatttt tctaaataca ttcaaaatat gtatccgctc atgagacaat
      841 aaccctgata aatgcttcat aatattgaaa aaggaagagt atgagtattc aacatttccg
      901 tgtcgccctt attccctttt ttgcggcatt ttgcct
//
