LOCUS       sequence-490898-        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-term GST tag E2 variant selected for LUBAC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  TITLE     Schulman lab plasmids 2025
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GST-tag LUBAC binding E2 variant L3LU-1
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..39
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(571..593)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(738..756)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        824..928
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catcctccaa aatcggatga aaacctgtat tttcagggat ccatgcgtgc ggcctggagg
       61 aggctgccgc gtgagcttga agaaatccgc aaagcaggga tgaaaaactt ccgtaacatc
      121 caggttgatg aagctaattt attgacttgg caagggctta ttgttcctga caaccctcca
      181 tatgataagg gagccttcag aatcgaaatc aactttccag cagagtaccc attcaaacca
      241 ccgaagatca catttaaaac aaagatctat cacccaaaca tcgacgaaaa ggggcaggtc
      301 tgtctgccag taattagtgc cgaaaactgg aagccagcaa ccaaaaccga ccaagtaatc
      361 cagtccctca tagcactggt gaatgacccc cagcctgagc acccgcttcg ggctgaccta
      421 gctgaagaat actctaagga ccgtaaaaaa ttcgcaaaga atgctgaaga gtttacaaag
      481 aaatatgggg aaaagcgacc tgtggactaa gcggccgcat cgtgactgac tgacgatctg
      541 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
      601 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
      661 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtataa
      721 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      781 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      841 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      901 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      961 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1021 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1081 cggtaagatc cttgagagtt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1141 gttctgctat gtggcgcgg
//
