LOCUS       sequence-49097-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HDHD1A
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             536..553
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg cggcgccccc gcagcccgtc acccacctca tctttgacat ggacggactt
      121 cttctggata ctgaacggct gtattcagtg gtgtttcaag aaatatgtaa tcgctatgac
      181 aagaaataca gctgggatgt aaagtccctg gttatgggta agaaggcatt agaggcggca
      241 cagattataa tagacgtctt gcagctcccg atgtccaaag aggagctggt ggaagaaagc
      301 caaacgaagt taaaggaagt gttccccacg gctgcgctca tgccaggggc ggagaaactc
      361 atcatccacc tgcggaaaca tggcatcccc tttgcactgg ccaccagctc gaggtccgcg
      421 tcgttcgata tgaagacaag ccgccacaag gagttcttca gcttgttttc ccacattgtg
      481 ctgggagatg accccgaagt gcagcatggc aagccagacc cagacatctt cctagcttgt
      541 gccaagaggt tctctccccc tcctgctatg gagaagtgcc ttgtctttga agatgctccc
      601 aatggggtgg aggcggccct ggcagctggg atgcaggtgg tcatggttcc tgacggaaac
      661 ttgagccgag atctgacaac aaaggccacc ctggtgctga attccctgca ggacttccag
      721 cccgagctgt ttggtttgcc ctcctatgag tgacagtaaa ggtggatacg gatccgaa
//
