LOCUS       sequence-491100-       10373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for PiggyBac mediated integration and stable expression of 
            hFGFR3c-N540K protein, mutant variant causes Hypochondroplasia and 
            Thanatophoric dysplasia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10373)
  TITLE     Krejci Lab plasmids
REFERENCE   2  (bases 1 to 10373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTR01F/hFGFR3c-N540K-V5
FEATURES             Location/Qualifiers
     source          1..10373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2830..2871
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2881..2898
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2949..3070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3318..3529
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3542..3810
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3839..3848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3869..4531
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4601..4654
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4655..5254
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4655..4674)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5151..5171
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5308..5328)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5370..5491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6254..6288
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6779..6795)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6779..6795)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6792..6814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6803..6819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6827..6833,6834..6851,6852..6857))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6872..6893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7010..7027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7181..7769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7261..7280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7940..8731,8732..8800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8563..8582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8801..8905)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8931..9386)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9068..9089)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9280..9299
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9513..9535
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9527..9544
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9528..9544
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9674..9736
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     10221..10240
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag cttggtaccg
      301 agctcggatc cagtaccctt cacccacgac cccaccatgg gcgcccctgc ctgcgccctc
      361 gcgctctgcg tggccgtggc catcgtggcc ggcgcctcct cggagtcctt ggggacggag
      421 cagcgcgtcg tggggcgagc ggcagaagtc ccgggcccag agcccggcca gcaggagcag
      481 ttggtcttcg gcagcgggga tgctgtggag ctgagctgtc ccccgcccgg gggtggtccc
      541 atggggccca ctgtctgggt caaggatggc acagggctgg tgccctcgga gcgtgtcctg
      601 gtggggcccc agcggctgca ggtgctgaat gcctcccacg aggactccgg ggcctacagc
      661 tgccggcagc ggctcacgca gcgcgtactg tgccacttca gtgtgcgggt gacagacgct
      721 ccatcctcgg gagatgacga agacggggag gacgaggctg aggacacagg tgtggacaca
      781 ggggcccctt actggacacg gcccgagcgg atggacaaga agctgctggc cgtgccggcc
      841 gccaacaccg tccgcttccg ctgcccagcc gctggcaacc ccactccctc catctcctgg
      901 ctgaagaacg gcagggagtt ccgcggcgag caccgcattg gaggcatcaa gctgcggcat
      961 cagcagtgga gcctggtcat ggaaagcgtg gtgccctcgg accgcggcaa ctacacctgc
     1021 gtcgtggaga acaagtttgg cagcatccgg cagacgtaca cgctggacgt gctggagcgc
     1081 tccccgcacc ggcccatcct gcaggcgggg ctgccggcca accagacggc ggtgctgggc
     1141 agcgacgtgg agttccactg caaggtgtac agtgacgcac agccccacat ccagtggctc
     1201 aagcacgtgg aggtgaacgg cagcaaggtg ggcccggacg gcacacccta cgttaccgtg
     1261 ctcaagacgg cgggcgctaa caccaccgac aaggagctag aggttctctc cttgcacaac
     1321 gtcacctttg aggacgccgg ggagtacacc tgcctggcgg gcaattctat tgggttttct
     1381 catcactctg cgtggctggt ggtgctgcca gccgaggagg agctggtgga ggctgacgag
     1441 gcgggcagtg tgtatgcagg catcctcagc tacggggtgg gcttcttcct gttcatcctg
     1501 gtggtggcgg ctgtgacgct ctgccgcctg cgcagccccc ccaagaaagg cctgggctcc
     1561 cccaccgtgc acaagatctc ccgcttcccg ctcaagcgac aggtgtccct ggagtccaac
     1621 gcgtccatga gctccaacac accactggtg cgcatcgcaa ggctgtcctc aggggagggc
     1681 cccacgctgg ccaatgtctc cgagctcgag ctgcctgccg accccaaatg ggagctgtct
     1741 cgggcccggc tgaccctggg caagcccctt ggggagggct gcttcggcca ggtggtcatg
     1801 gcggaggcca tcggcattga caaggaccgg gccgccaagc ctgtcaccgt agccgtgaag
     1861 atgctgaaag acgatgccac tgacaaggac ctgtcggacc tggtgtctga gatggagatg
     1921 atgaagatga tcgggaaaca caaaaacatc atcaagctgc tgggcgcctg cacgcagggc
     1981 gggcccctgt acgtgctggt ggagtacgcg gccaagggta acctgcggga gtttctgcgg
     2041 gcgcggcggc ccccgggcct ggactactcc ttcgacacct gcaagccgcc cgaggagcag
     2101 ctcaccttca aggacctggt gtcctgtgcc taccaggtgg cccggggcat ggagtacttg
     2161 gcctcccaga agtgcatcca cagggacctg gctgcccgca atgtgctggt gaccgaggac
     2221 aacgtgatga agatcgcaga cttcgggctg gcccgggacg tgcacaacct cgactactac
     2281 aagaagacaa ccaacggccg gctgcccgtg aagtggatgg cgcctgaggc cttgtttgac
     2341 cgagtctaca ctcaccagag tgacgtctgg tcctttgggg tcctgctctg ggagatcttc
     2401 acgctggggg gctccccgta ccccggcatc cctgtggagg agctcttcaa gctgctgaag
     2461 gagggccacc gcatggacaa gcccgccaac tgcacacacg acctgtacat gatcatgcgg
     2521 gagtgctggc atgccgcgcc ctcccagagg cccaccttca agcagctggt ggaggacctg
     2581 gaccgtgtcc ttaccgtgac gtccaccgac gagtacctgg acctgtcggc gcctttcgag
     2641 cagtactccc cgggtggcca ggacaccccc agctccagct cctcagggga cgactccgtg
     2701 tttgcccacg acctgctgcc cccggcccca cccagcagtg ggggctcgcg gacggccaag
     2761 ggtcaagaca attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg
     2821 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     2881 catcatcacc atcaccattg agtttaaacc ctagagtcgg ggcggccggc cgcttcgagc
     2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtt
     3181 tgcgtattgg gcgctcttcc gctgatctgc gcagcaccat ggcctgaaat aacctctgaa
     3241 agaggaactt ggttagctac cttctaaatc ggatccgcgg ccgcgaagga tctgcgatcg
     3301 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3361 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3421 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     3481 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     3541 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     3601 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     3661 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     3721 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     3781 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc
     3841 caccatggag agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg
     3901 caccctgaac ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg
     3961 ccgcatgacc aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct
     4021 gagccacgtg atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa
     4081 ccccttcctg cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga
     4141 ggacggcggc gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg
     4201 cgacttcaag gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat
     4261 catccgcagc aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg
     4321 cagcttcgcc cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag
     4381 ccacatgcac ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt
     4441 cgccttccgc cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca
     4501 gcacgccttc aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc
     4561 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct
     4621 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     4681 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4741 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4801 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4861 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4921 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4981 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     5041 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     5101 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     5161 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     5221 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     5281 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5341 tttaatgcct ttgtatcatg cgttaactaa acttgtttat tgcagcttat aatggttaca
     5401 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5461 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gaattgactc aaatgatgtc
     5521 aattagtcta tcagaagctc atctggtctc ccttccgggg gacaagacat ccctgtttaa
     5581 tatttaaaca gcagtgttcc caaactgggt tcttatatcc cttgctctgg tcaaccaggt
     5641 tgcagggttt cctgtcctca caggaacgaa gtccctaaag aaacagtggc agccaggttt
     5701 agccccggaa ttgactggat tcctttttta gggcccattg gtatggcttt ttccccgtat
     5761 ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg
     5821 tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga
     5881 gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg
     5941 taattacatc cctgggggct ttgggggggg gctgtccctg atatctataa caagaaaata
     6001 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
     6061 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
     6121 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
     6181 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
     6241 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg
     6301 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
     6361 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     6421 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     6481 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     6541 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
     6601 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     6661 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
     6721 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt
     6781 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6841 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6901 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6961 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7021 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7621 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8221 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8401 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8461 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8521 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8581 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8641 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8701 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8761 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8821 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8881 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     8941 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     9001 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     9061 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     9121 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     9181 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     9241 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     9301 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     9361 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     9421 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     9481 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     9541 cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc agactcgcgg
     9601 tgcaaatgtg ttttacagcg tgatggagca gatgaagatg ctcgacacgc tgcagaacac
     9661 gcagctagat taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgt
     9721 gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat taatttgaat
     9781 agatattaag ttttattata tttacactta catactaata ataaattcaa caaacaattt
     9841 atttatgttt atttatttat taaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     9901 aaaactttta tgagggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc
     9961 cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc
    10021 atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc
    10081 tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga
    10141 aaaggcctcc acggccacta gtattatgcc cagtacatga ccttatggga ctttcctact
    10201 tggcagtaca tctacgtaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca
    10261 gaacatttct ctggcctaac tggccggtac ctgagctcgc tagcgggaat ttccggggac
    10321 tttccgggaa tttccgggga ctttccggga atttccagat cgctattacc atg
//
