LOCUS       sequence-491240-       11640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses bovine MSTN (p.L64P) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11640)
  TITLE     pcDNA3.1-MSTN expression plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1-MSTN-L64P
FEATURES             Location/Qualifiers
     source          1..11640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7231..7248)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7237..7461
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7507..7935
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7594..7613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7804..7825
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7944..7964)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7949..8278
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8129..8264
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8191..8210
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8345..9139
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8399..8418)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9009..9028
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9313..9434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9350..9369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9404..9423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9483..9499)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9483..9499)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9496..9518)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9507..9523
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9531..9537,9538..9555,9556..9561))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9576..9597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9714..9731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9885..10473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9965..9984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10644..11435,11436..11504))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11267..11286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11505..11609)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgtttgg cttggcgtta ctcaaaagca
      961 aaagaaaagt aaaaggaaga agtaagaaca agggaaaaga ttgtattgat tttaaaacca
     1021 tgcaaaaact gcaaatctct gtttatattt acctatttat gctgattgtt gctggcccag
     1081 tggatctgaa tgagaacagc gagcagaagg aaaatgtgga aaaagagggg ctgtgtaatg
     1141 catgtttgtg gagggaaaac actacatcct caagactaga agccataaaa atccaaatcc
     1201 tcagtaaacc tcgcctggaa acagctccta acatcagcaa agatgctatc agacaacttt
     1261 tgcccaaggc tcctccactc ctggaactga ttgatcagtt cgatgtccag agagatgcca
     1321 gcagtgacgg ctccttggaa gacgatgact accacgccag gacggaaacg gtcattacca
     1381 tgcccacgga gtgtgagtag tcctgctggt gcagagcaac gactctgctg actgctgttc
     1441 tagtgttcat gagaaaccga tctattttca ggctctttta acaagctgct ggcttgtatg
     1501 taaggaggag gggaaagagc ttttttcaag atttcatgag aaatagacca atgagactga
     1561 aagctgctac tttatttgtt tccttagaga gctaaaaagc taaaaatcaa aaatgaaatg
     1621 cttgcatagc attcatgtta tatagtttag tatgacaact ataacatgtt tatgttttca
     1681 cagcttaatg ctaccaaggt aaaggattgg gagacagtat cagcaatgtg aaaaatttac
     1741 atcaaatttc ctaattgcat ttggttgcct gaaatatgca tttaaaataa cagggtgttg
     1801 tttttttttt tttttttttc attaataaaa gagaaaggaa gaaatctgta gaggttgaag
     1861 cctatctggg cgtttgctga acacttagaa tgacttctgt tattcaaaac tatttctcat
     1921 agggttttta tggtcttcac agagtatcta attttgaaag ctattagagt ggaaaggata
     1981 aaagaatatt cttaataaac ttaatgtatt agtaagagca ataaggaagt aaacacagca
     2041 tagtgaaaaa tcatgagcta atcagcagaa aattctaaga aataaacatt ttaattacaa
     2101 agttccactt ataccctgac catggtacta ttgttgagag tacctggtct gcacatatct
     2161 aggaggcaca tgcttaataa ccttctaaaa tattattgta ttcctcatag gagggagaac
     2221 tattacctat atgtagtacc tatgttgttt ctgaaagata atatgtttca tgtatttctg
     2281 ttgcagtcac ttcaaaccta tactcaagga aagggagaca ggcatctcaa cagagaaggc
     2341 atgaccagaa agagttttgt gccatgtgtc tgcgatcttg ctttatacag ggctctaccc
     2401 actttaaact ggactcaaaa cagtttcaaa atactgcttt ttcttattaa gtaactagtt
     2461 tataaggcaa caaataaatt tcctttaaga ctgtgctatc agataatcct ggaatagatt
     2521 tgccttactt ataaacaatc ttgagaaaac aaaaaggcaa gaaattgcta agtgcttctg
     2581 cttacaatga cagcctggcc ctaaagacaa tgttttctaa gttttgaaac agcttgaata
     2641 caacatctaa gttttggtgc taattacctg ctagtttttt tatttttttc ctttaaaagg
     2701 ctgtcccagc gtcctaacat aacagatgca ctatattttc tgctaattcc cgaggctcag
     2761 ttagttgctc actgtgtctt gtccccaggt aattcaggcc tgggggaagg gttccttcct
     2821 ccagactgat tggtacagct gctcagtaag tgtaactact cagattccca aagaattcta
     2881 agtggatgtt cttccacagt gtctcttgtt ctctctaatc atcatcattt taaaatttca
     2941 tccacttttc attccttaat agaattttcc ttagtccaca gttctctgga aaggaagtag
     3001 gcttctcata acagctgaaa aaacatatac ctaaaagatt ctgaaaagct gtaataactg
     3061 ttatacttga tattttgctg ttatgaatga aatgctacat atttttccat tttaaaagac
     3121 taaatatgca cacattattc caattaaaaa atgttcatag attgatatgg aggtgttcgt
     3181 tcatttttca taaaaatgat cttagtaact ttttttctta ttcatttata gctgatcttc
     3241 taacgcaagt ggaaggaaaa cccaaatgtt gtttctttaa atttagctct aagatacaat
     3301 acaataaact agtaaaggcc caactgtgga tatatctgag gcctgtcaag actcctgcga
     3361 cagtgtttgt gcaaatcctg agactcatca aacccatgaa agacggtaca aggtatactg
     3421 gaatccgatc tctgaaactt gacatgaacc caggcactgg tatttggcag agcattgatg
     3481 tgaagacagt gttgcagaac tggctcaaac aacctgaatc caacttaggc attgaaatca
     3541 aagctttaga tgagaatggc catgatcttg ctgtaacctt cccagaacca ggagaagatg
     3601 gactggtaag tgattactga aaataacatg ctaaaaacct tgttatgtgt ttattcataa
     3661 tgtgaatgaa tagtagtgaa aaataactac cagtttcctg tgcttataag ccagacaaag
     3721 gcaccttacc ccagtggtag ccctgtactc aataaaagta ggtgtcccat ttcacatcct
     3781 atgaaacact ctcttgatac tttgactttg catgaggatt taaaagaaaa aaagttatac
     3841 catggtcctt aagtttttag ggaattcttt ggaattgaga atgaaatata aaatgctttc
     3901 cgttgatgtg ctacatgatt atataaataa aaacatgaag tcttcacagt ggattctagt
     3961 actcacccaa caacacattt tttcccccag aagagtgacc aatttgttaa aattcttttg
     4021 cttaataagg cagaaaaatg aactctacaa gttataatta aaataaaatg cttttactta
     4081 tagaaattaa ctagatatat gttcaggttt atatactatt aaatatacta tatttaagat
     4141 ctctcatgat aaatatgttc cttgttttat agactattga tgcactgatg tatatgtgga
     4201 ttactttgtg aattacccct ggtaaaatta aaaatttcag gctagttaac ttgtactact
     4261 tagctatttt ctgaactgtc ttactgttct ttaacaggag ttaacttagg taatgtcaac
     4321 taatttaata taaagtcaaa cagaaaataa tgccttatat attataaaaa ttaataaaaa
     4381 accattttaa aatctagtat aagtttagag ctactcactc ttctggctta tctatgcttg
     4441 tatttacttc tgttttcaaa aaatttttta atgtgaccat accttttatt tccagttatt
     4501 gatataattt acaacaaaag attatacttg caagctttat agtttttaaa tggtcttatt
     4561 tgtagtgaat atcatatcta aatgatatct aaatgtaaag taaatcatac ctaaatgaaa
     4621 acatattctt taagtcatta taaaattttc caggtgatca atttttcttt aaatatacta
     4681 cataaaatgt tattgactcc caaaatgatg ttattttgta taatcttaaa taccaataat
     4741 taccaggtct attttggttt tagtgtagga taaaaaagaa tgtgttcttt tttctaggta
     4801 gcattttaat gatcaaagtt ggtgacgtga cagaggtctt aagtattatt aaacagatga
     4861 ttaataagat gtattcctca gacttttcca tataaaagga aaaatgtctc aaattcatga
     4921 aaagattggt acaggaggag gattagcaaa ttgtagttta aatatctgaa tggaaacact
     4981 ttttagtgaa agaataaagg gaatatcatt gtatcttctt ctgagtctgt gcctctctct
     5041 cttggagtta gtctttccaa ccctatatac ttaccactat cttcatccct ctaccttcct
     5101 ttttcccatt acatctgtgc agtactgggt ggcaactatt gtgtttcggt gttaatatcc
     5161 aagtttccct gaataagacc aagtgaatgg aggatgaatg agtataccta tccctccagg
     5221 ggtcatcaga catatttagc caccatattt aatcaataag caggaagaca taagctagcc
     5281 ttgtccttct tctttcctcc ctgctccttt ctcttctctt ccccctctcc ctttactgtc
     5341 atccatcagt attttcagag catctattat gtgtcaggca ttcagatact caaacggagg
     5401 aaaacaagaa taaacaagac aaagatctga ccacagggga atccctatgg ctactgtaga
     5461 cttttgagcc ataaaggaag aatcaagcct agtgtaaatg aaaattcctt aatgctgtgc
     5521 cttttaaaaa gaaatgtgac ataagcaaaa tgattagttt ctttctttaa taatgagtcc
     5581 ttgaggtagg agagtgtttt gggatctatt attaactctt ctttcctttc catacagact
     5641 ccttttttag aagtcaaggt aacagacaca ccaaaaagat ctaggagaga ttttgggctt
     5701 gattgtgatg aacactccac agaatctcga tgctgtcgtt accctctaac tgtggatttt
     5761 gaagcttttg gatgggattg gattattgca cctaaaagat ataaggccaa ttactgctct
     5821 ggagaatgtg aatttgtatt tttgcaaaag tatcctcata cccatcttgt gcaccaagca
     5881 aaccccagag gttcagccgg cccctgctgt actcctacaa agatgtctcc aattaatatg
     5941 ctatatttta atggcgaagg acaaataata tacgggaaga ttccagccat ggtagtagat
     6001 cgctgtgggt gttcatgagg tctatatttg gttcatagct tcctcaaaca tggaaggtct
     6061 tcccctcaac aattttgaaa ctgtgaaatt atgtaccaca ggctataagc ctagagtatg
     6121 ctacagtcac ttaagcacaa gctacagtat atgagctaaa aagagagaat atatgcaatg
     6181 gttggcattt aaccatccaa acaaatcgta taataaaaag ttttatgatt tccagagttt
     6241 ttgaactagg agatcaaatt ccatttatgt tgaaatatat tacaacacat gcaggtgaat
     6301 gaaagcaatt ctccttgtct tctggtgaat taaaggagta tgctttaaaa tctatttctc
     6361 tacagtttca cttaatattt acagaaaaat ctatatgtag tattggtaaa atgcagtatt
     6421 gttatatacc attatttgaa acatccttaa acacttgaat ttatattgta tgatagcata
     6481 cttggtaaga tgagattcca caaagtaggg atggcacacc atacgcaggt taccattcct
     6541 atactgattg atacagtaca ttaacagttt ttgccaatgg tgctaataca ataggctgaa
     6601 tggctgatgt tatcaggttt atcaagcaaa aaacattcag gaaagtaata agtttctcct
     6661 ttcttcaggt gcattttcac actcctccct atgggcaatg gatgttctat agagaaagaa
     6721 aactcatttt cctagaggtc tacattcaat tctgtagcat acttggagaa gctgcattga
     6781 aaaggcagtc aaaaagtatt cattttggtc aaaatttcaa aattatagcc tgcctttgca
     6841 atactgcagc ttttaggatg aaataatgga aatgactgat tctatcaata ttgtataaaa
     6901 agattttgaa atagttgcat ttatataata tgtatacaat attgttttgt aaataaatgt
     6961 ctcctttttt atttactttg gtatattttt acagtaagga catttcaaat taagtattaa
     7021 ggcacaaaga catgtcatgt aggacataaa agcaaaagct tatattttgg agcaaattag
     7081 ttgattaaat agtggtctta aaactccata tgctaatggt tagatggtta tattacaatc
     7141 attttatatt tttttacatt attagcattc acttatggat tcatgatggc tgtctcgagt
     7201 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     7261 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     7321 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7381 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7441 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     7501 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7561 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7621 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7681 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7741 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7801 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7861 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7921 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7981 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8041 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8101 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8161 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     8221 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8281 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     8341 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     8401 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     8461 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     8521 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     8581 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     8641 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     8701 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     8761 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     8821 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     8881 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     8941 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     9001 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     9061 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     9121 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     9181 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     9241 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     9301 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9361 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9421 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     9481 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9541 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9601 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9661 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    10381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    11041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    11101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    11161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    11221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    11281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    11341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    11401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    11461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
