LOCUS       sequence-49134-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSP90B1
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             787..807
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             808..825
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             826..849
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgaagacca agcctatttg gaccagaaac cctgatgaca tcacccaaga ggagtatgga
       61 gaattctaca agagcctcac taatgactgg gaagaccact tggcagtcaa gcacttttct
      121 gtagaaggtc agttggaatt cagggcattg ctatttattc ctcgtcgggc tccctttgac
      181 ctttttgaga acaagaagaa aaagaacaac atcaaactct atgtccgccg tgtgttcatc
      241 atggacagct gtgatgagtt gataccagag tatctcaatt ttatccgtgg tgtggttgac
      301 tctgaggatc tgcccctgaa catctcccga gaaatgctcc agcagagcaa aatcttgaaa
      361 gtcattcgca aaaacattgt taagaagtgc cttgagctct tctctgagct ggcagaagac
      421 aaggagaatt acaagaaatt ctatgaggca ttctctaaaa atctcaagct tggaatccac
      481 gaagactcca ctaaccgccg ccgcctgtct gagctgctgc gctatcatac ctcccagtct
      541 ggagatgaga tgacatctct gtcagagtat gtttctcgca tgaaggagac acagaagtcc
      601 atctattaca tcactggtga gagcaaagag caggtggcca actcagcttt tgtggagcga
      661 gtgcggaaac ggggcttcga ggtggtatat atgaccgagc ccattgacga gtactgtgtg
      721 cagcagctca aggaatttga tgggaagagc ctggtctcag ttaccaagga gggtctggag
      781 ctggcagaga acctctactt ccaatcgcac catcatcacc accatgatta caaggatgac
      841 gacgataagt gaggatcc
//
