LOCUS       sequence-491879-       11263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse IL-10 and BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Simic MS, Watchmaker PB, Gupta S, Wang Y, Sagan SA, Duecker J, 
            Shepherd C, Diebold D, Pineo-Cavanaugh P, Haegelin J, Zhu R, Ng B, 
            Yu W, Tonai Y, Cardarelli L, Reddy NR, Sidhu SS, Troyanskaya O, 
            Hauser SL, Wilson MR, Zamvil SS, Okada H, Lim WA
  TITLE     Programming tissue-sensing T cells that deliver therapies to the 
            brain.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4237. doi: 
            10.1126/science.adl4237. Epub 2024 Dec 6.
  PUBMED    39636984
REFERENCE   2  (bases 1 to 11263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4237. doi"; pages: " 
            10.1126/science.adl4237. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR_PGK_BFP_2A-mIL10
FEATURES             Location/Qualifiers
     source          1..11263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    254..379
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    876..1109
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1294..1338
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1623..1740
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1893..2300
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2428..2457
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3799..3855
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3856..3858,3859..3861,3862..4572)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4162..4181)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    4700..5288
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4753..4773)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5171..5182)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5291..5307)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5292..5308)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5398..5631
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5656..5674)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5657..5674)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5964..5983)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6197..6216)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6316..6338
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6376..6394)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6462..6566
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6567..6635,6636..7427)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6785..6804)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7598..8186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8087..8106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8340..8357
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8427..8447)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8432..8761
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8612..8747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9895..9960
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10090..10110
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10535..10669
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10572..10591)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10626..10645
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10837..11263,1..207)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
       61 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      121 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      181 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      241 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      301 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      361 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      421 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
      481 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
      541 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
      601 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
      661 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
      721 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
      781 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
      841 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
      901 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
      961 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1021 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1081 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1141 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1201 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     1261 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     1321 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     1381 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     1441 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     1501 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     1561 agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac
     1621 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1681 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1741 cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc
     1801 ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     1861 accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag gcatggaaaa
     1921 ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa tagctaacgt
     1981 tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc caagaacaga
     2041 tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc agatatggcc
     2101 caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca aggacctgaa
     2161 atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc
     2221 ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg ccagtcctcc
     2281 gacagactga gtcgcccggg ggggatctgg agctctcgag aattctcacg cgtcaagtgg
     2341 agcaaggcag gtggacagtg gatcatggcc ttaccagtga ccgccttgct cctgccgctg
     2401 gccttgctgc tccacgccgc caggccggag cagaagctga tcagcgagga ggacctggac
     2461 atccagatga cacagactac atcctccctg tctgcctctc tgggagacag agtcaccatc
     2521 agttgcaggg caagtcagga cattagtaaa tatttaaatt ggtatcagca gaaaccagat
     2581 ggaactgtta aactcctgat ctaccataca tcaagattac actcaggagt cccatcaagg
     2641 ttcagtggca gtgggtctgg aacagattat tctctcacca ttagcaacct ggagcaagaa
     2701 gatattgcca cttacttttg ccaacagggt aatacgcttc cgtacacgtt cggagggggg
     2761 accaagctgg agatcacagg tggcggtggc tcgggcggtg gtgggtcggg tggcggcgga
     2821 tctgaggtga aactgcagga gtcaggacct ggcctggtgg cgccctcaca gagcctgtcc
     2881 gtcacatgca ctgtctcagg ggtctcatta cccgactatg gtgtaagctg gattcgccag
     2941 cctccacgaa agggtctgga gtggctggga gtaatatggg gtagtgaaac cacatactat
     3001 aattcagctc tcaaatccag actgaccatc atcaaggaca actccaagag ccaagttttc
     3061 ttaaaaatga acagtctgca aactgatgac acagccattt actactgtgc caaacattat
     3121 tactacggtg gtagctatgc tatggactac tggggccaag gaacctcagt caccgtctcc
     3181 tcaaccacga cgccagcgcc gcgaccacca acaccggcgc ccaccatcgc gtcgcagccc
     3241 ctgtccctgc gcccagaggc gtgccggcca gcggcggggg gcgcagtgca cacgaggggg
     3301 ctggacttcg cctgtgatat ctacatctgg gcgcccttgg ccgggacttg tggggtcctt
     3361 ctcctgtcac tggttatcac cctttactgc ggttctggct ccggatcagg atctaagcgg
     3421 ggcagaaaga agaacttcca caactttctg gcctggccgt ttcctaatct gcctcctctg
     3481 gaagccatgg acatggtgga agtgatctac tacaaccgga agaagaaagt ctgggactac
     3541 ggctccggat caggatctaa gcggagaggc agagatcctg agatgggcgg caagcccaga
     3601 cggaagaatc ctcaagaggg cctgtataat gagctgcaga aagacaagat ggccgaggcc
     3661 tacagcgaga tcggaatgaa gggcgagcgc agaagaggca agggacacga tggactgtac
     3721 cagggcctga gcaccgccac caaggatacc tatgatgccc tgcacatgca ggccctgcct
     3781 ccaagatctg gaagcggcgc cacaaacttc agcctgctga aacaggctgg cgacgtggaa
     3841 gagaaccccg gacctatggt gtccaagggc gaagaactgt tcaccggcgt ggtgcccatt
     3901 ctggtggaac tggacgggga tgtgaacggc cacaagttta gcgttagcgg cgaaggcgaa
     3961 ggggatgcca catacggaaa gctgaccctg aagttcatct gcaccaccgg caagctgcct
     4021 gtgccttggc ctacactggt caccacactg acatacggcg tgcagtgctt tagcagatac
     4081 cccgaccata tgaagcagca cgacttcttc aagagcgcca tgcctgaggg ctacgtgcaa
     4141 gagcggacca tcttctttaa ggacgacggc aactacaaga ccagggccga agtgaagttc
     4201 gagggcgaca ccctggtcaa ccggatcgag ctgaagggca tcgacttcaa agaggacggc
     4261 aacatcctgg gccacaagct cgagtacaac tacaacagcc acaacgtgta catcatggcc
     4321 gacaagcaga aaaacggcat caaagtgaac ttcaagatcc ggcacaacat cgaggacggc
     4381 tctgtgcagc tggccgatca ctaccagcag aacacaccca tcggagatgg ccctgtgctg
     4441 ctgcccgata accactacct gagcacacag agcgccctga gcaaggaccc caacgagaag
     4501 agggatcaca tggtgctgct ggaattcgtg accgccgctg gcatcacact cggcatggac
     4561 gagctgtata agtgagatcc ggcagtggta gatccttgac ttgcggccgc aactcccacc
     4621 tgcaacatgc gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct
     4681 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4741 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4801 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4861 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4921 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4981 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     5041 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     5101 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     5161 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5221 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5281 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
     5341 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5401 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     5461 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5521 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5581 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
     5641 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     5701 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     5761 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     5821 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
     5881 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     5941 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     6001 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     6061 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     6121 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     6181 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     6241 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     6301 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     6361 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     6421 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6481 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6541 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6601 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6661 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6721 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6781 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6841 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6901 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6961 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7021 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7081 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7141 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7201 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7261 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7321 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7381 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7441 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7501 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7561 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7621 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7681 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7741 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7801 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7861 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7921 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7981 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8041 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8101 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8161 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8221 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8281 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8341 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8401 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8461 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8521 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8581 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8641 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8701 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8761 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     8821 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     8881 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     8941 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     9001 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     9061 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     9121 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     9181 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     9241 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     9301 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     9361 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     9421 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     9481 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     9541 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     9601 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     9661 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     9721 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     9781 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     9841 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     9901 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     9961 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
    10021 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
    10081 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
    10141 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
    10201 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
    10261 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
    10321 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
    10381 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
    10441 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
    10501 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
    10561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    10681 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    10741 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    10801 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
    10861 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
    10921 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
    10981 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
    11041 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
    11101 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
    11161 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
    11221 gcctgggcgg gactggggag tggcgagccc tcagatcctg cat
//
