LOCUS       sequence-492314-       10817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pL1-R2-pLsUBI-RUBY-tLsUBI (Golden Gate Level 1 module with a 
            transcription unit expressing RUBY from the LsUBI promoter with the 
            tLsUBI terminator for R2 position in Level 2 vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10817)
  TITLE     Monitoring the stability of transgene expression in lettuce using 
            the RUBY reporter.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pR2B6
FEATURES             Location/Qualifiers
     source          1..10817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    105..129
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      221..852
                     /label=oriV
                     /note="incP origin of replication"
     promoter        939..1043
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1044..1112,1113..1904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1262..1281)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2075..2663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2564..2583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2999..4147
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4303..4327
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6261..10211
                     /label=RUBY
     CDS             7761..7817
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8652..8708
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aagcttgaat tcttgcttgt cttcacagag tggggcccac tgcatccacc ccagtacatt
       61 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
      121 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
      181 gatacaggca gcccatcagt cagatcagga tctcctttgc gacgctcacc gggctggttg
      241 ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga
      301 agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc
      361 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt
      421 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa
      481 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga
      541 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat
      601 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg
      661 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc
      721 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg
      781 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc
      841 tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
      901 ggcctcaccc caaaaatggc agcgctggcc aattcgtgcg cggaacccct atttgtttat
      961 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccttga taaatgcttc
     1021 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1081 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1141 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1201 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1261 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1321 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1381 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1441 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1501 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1561 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1621 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1681 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1741 ctggagccgg tgagcgtggt tctcgcggta tcattgcagc actggggcca gatggtaagc
     1801 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1861 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1921 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1981 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2041 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2101 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2161 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2221 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2281 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2341 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2401 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2461 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2521 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2581 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2641 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcag
     2701 atcctagatg tggcgcaacg atgccggcga caagcaggag cgcaccgact tcttccgcat
     2761 caagtgtttt ggctctcagg ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt
     2821 attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga cggtctacgg
     2881 gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag gcgggtcaaa
     2941 tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag gagggtgaat
     3001 gaatcggacg tttgaccgga aggcatacag gcaagaactg atcgacgcgg ggttttccgc
     3061 cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca
     3121 gtccgtcggc tcgatggtcc agcaagctac ggccaagatc gagcgcgaca gcgtgcaact
     3181 ggctccccct gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc gtcttgaaca
     3241 ggaggcggca ggtttggcga agtcgatgac catcgacacg cgaggaacta tgacgaccaa
     3301 gaagcgaaaa accgccggcg aggacctggc aaaacaggtc agcgaggcca agcaggccgc
     3361 gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag ctttccttgt tcgatattgc
     3421 gccgtggccg gacacgatgc gagcgatgcc aaacgacacg gcccgctctg ccctgttcac
     3481 cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa
     3541 caaggacgtg aagatcacct acaccggcgt cgagctgcgg gccgacgatg acgaactggt
     3601 gtggcagcag gtgttggagt acgcgaagcg cacccctatc ggcgagccga tcaccttcac
     3661 gttctacgag ctttgccagg acctgggctg gtcgatcaat ggccggtatt acacgaaggc
     3721 cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg
     3781 gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac
     3841 gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta
     3901 cacgaaattc atatgggaga agtaccgcaa gctgtcgccg acggcccgac ggatgttcga
     3961 ctatttcagc tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg
     4021 cggatcggat tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga
     4081 gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa
     4141 acgctagggc cttgtggggt cagttccggc tgggggttca gcagccagcg cctgatctgg
     4201 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
     4261 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
     4321 cctgtcaaac actgatagtt taaaccactt cgtgcagaag acaatagtgg agcggttttt
     4381 cagtgacctc tctaaagtga tatattcccg acaaaatggt tattttacaa aaaaaataat
     4441 catttttttt caaattattc atttatatgg gttagaatac tcaatttccc tgtgttattt
     4501 aatttaaaat catttccaat atacaaacat actttattat ctgattattt ataataaatt
     4561 ttcataaagt ttcacctaat cttttattcc ttatattata aaatttatcc atccaaatca
     4621 aacttatttt gtataattta aaagtatttt caatataatc atttggtttg tggtgacgta
     4681 gtaaaacata tgaaatatat gatgtataac ttattgatca atgttcaaat tttgacacta
     4741 ctcggtcctg tggccataaa tgtttgtcgg cattgactcc aaaacatgaa gtatcctctt
     4801 taataaatta aagttttttt ttgtcacttg tcacactctc atgcattata caaaatgtca
     4861 ttttgtcata attttgagat atttattttt catttgtcat ttacttcact ttttattttt
     4921 gaatatatcc ttaatttagt ttttataaat taaccatatc ataataacta tattaatttt
     4981 aaattttgaa tttcaaactt caatcgaaac tttaaataaa tttacaattg aaacgtttgt
     5041 atttaacatt ttgttataat tagacatttg atacacgagt cagataacta aacacataat
     5101 tttaatttat ttttttcatt ttttttttat aattcttact tattccaaat ttcaaattca
     5161 aataaatttg ctgtttaatc attcgtattt aacattttat tgtaatcaac ccgtataata
     5221 tacaagtatc acaactacta acctcatatt tgcagcgaaa gattattcgg cgtgcaaaag
     5281 cagggcaaaa aaacccttgt tagaaagttt cttttccaaa ataaaaaatt aaaaaataaa
     5341 agtattttca atatcaataa catactctat tctctaatta tttattacta acttttcaaa
     5401 gagattaacc taatcttgta ttccttaaat tataaaattt acaaattata tggatccaaa
     5461 accaaatatc ccatcgactc aagatttaag ttaaaagttt cttggacgaa acacaaagaa
     5521 aagatattat cctaacccaa aagatgacgg cagccttaga aatcaccgcc actgattaaa
     5581 tatcggaatc caacgactct aatttcagac ccttcatcaa ctttttattg acacgtgtca
     5641 tattttcgtc cgtttgttat ctaacaaccc gtagcgttag ccttcctttt cgtgaagaaa
     5701 ccttttctta tcctataaat ccacccccaa atccatcatc ttccttcatc ctgcaatcat
     5761 ctttatcttc aaagctctga cttcttcaat tctcccaagg tattatcttt tttattcttt
     5821 tatgtttgtt tagtttttga acagtttatt ttcaatcgat taaaggttat tattgttttt
     5881 gatctgtttg tggcttttga tttaagatat aacctgatgt tgtaattaac acgattgata
     5941 gtgtcttaat atgttttaat tatggtttct agatctaatc atcgaattta ttaacgatct
     6001 gttatagttt cgtgttttcg atcgtttttc aacaagtcaa ttccaaaatc gatgttattg
     6061 tataaaagct tcgaacgacg attttttgta aattaattca cgatctaagt tttcaatttc
     6121 agagaataaa gttgttgatt tgtttttgaa ttgtaatctg atctctgttt caagtacgat
     6181 atgaatttgt ttgttgatga attgatgata aaactgtttt cgttttctaa taattgtttc
     6241 gttctttttt taacagaatg atggatcatg cgaccctcgc catgatcctc gcgatctggt
     6301 tcatcagctt ccacttcatc aagctgctgt tctcccagca gaccaccaag ctgcttccgc
     6361 caggaccaaa gccgcttccg atcatcggca acatccttga ggtgggcaag aagccgcatc
     6421 ggtccttcgc caacctcgcc aagattcacg gcccactcat ttccctcaga ctcggctctg
     6481 tgaccaccat cgttgtgtcc tctgccgacg tggccaaaga gatgttcctc aagaaggatc
     6541 acccgctctc caaccgcacg atcccgaata gtgttacagc cggcgaccac cacaagctca
     6601 ccatgtcttg gctcccggtg tctccgaagt ggcgcaactt ccgcaagatt accgccgtgc
     6661 atctgctctc cccacagaga ctcgatgcct gccagacatt caggcacgcc aaggtgcagc
     6721 agctctacga gtacgttcaa gagtgcgccc agaaaggcca ggccgtggat attggcaagg
     6781 ccgcctttac gaccagcctc aacctcctca gcaagctgtt cttcagcgtc gagctggcgc
     6841 accacaagtc ccataccagc caagagttca aagagctgat ctggaacatc atggaagata
     6901 taggcaagcc gaactacgcc gactacttcc cgattctcgg ctgcgttgac ccatctggca
     6961 ttagaagaag gctcgcctgc tccttcgaca agctgatcgc cgtgttccag ggcatcatct
     7021 gcgagagact cgccccagat tcctccacca caactaccac caccaccgac gacgtgctcg
     7081 atgtgctcct ccagctgttc aagcagaacg agctgacgat gggcgagatc aaccacctcc
     7141 tcgtggacat cttcgacgcc ggcaccgata ccacatcctc cacattcgag tgggtgatga
     7201 ccgagctgat ccgcaatcca gagatgatgg aaaaggccca agaggaaatc aagcaggtcc
     7261 tcggcaagga caagcagatc caagagtccg acatcatcaa cctgccgtac ctccaggcga
     7321 tcatcaaaga gacactccgc ctccatccgc cgaccgtgtt cttgctccca agaaaggccg
     7381 acaccgatgt cgagctgtac ggctacatcg tgccgaagga tgcccagatc ctcgtgaacc
     7441 tctgggccat tggcagggac ccaaacgcct ggcagaacgc cgatattttc agcccagagc
     7501 gcttcatcgg ctgcgagatc gatgttaagg gccgcgattt cggcctcctt ccatttggcg
     7561 ctggccgcag aatttgccca ggcatgaatc tcgccatcag gatgctcacc ctcatgctcg
     7621 ccacactcct ccagttcttc aactggaagc tcgaaggcga catctccccg aaggacctcg
     7681 acatggacga gaagttcggc attgcgctcc aaaagaccaa gccgctcaag ctcatcccga
     7741 ttccgcgcta cggtagcgga gctaccaatt ttagcctcct taagcaggca ggtgatgtag
     7801 aagagaaccc cgggcctatg aagatgatga acggcgagga cgccaacgac cagatgatca
     7861 aagagtcctt cttcatcacc cacggcaacc cgatcctcac cgtcgaggat acacatccgc
     7921 tcaggccgtt cttcgagaca tggcgcgaga agattttctc caagaagccg aaggccatcc
     7981 tcatcatctc cggccactgg gagacagtga agccaaccgt gaacgccgtg cacatcaacg
     8041 acaccatcca cgacttcgac gactacccag ccgccatgta ccagttcaag tacccagctc
     8101 caggcgagcc agagcttgcg agaaaggtgg aagagatcct caagaagtcc gggttcgaga
     8161 cagccgagac agaccaaaag aggggccttg atcacggcgc ctgggttcca ctcatgctca
     8221 tgtatccaga ggcggacatc ccggtgtgcc agctctcagt tcagccacat ctcgacggca
     8281 cctaccacta caatctcggc agagccctcg cgccgctcaa gaatgatggc gtgctcatta
     8341 ttggctccgg cagcgccaca catccactcg atgagacacc gcactacttc gatggtgttg
     8401 ccccttgggc cgctgccttc gattcttggc ttaggaaggc cctcatcaac ggccgcttcg
     8461 aggaagtgaa catctacgag agcaaggccc cgaactggaa gctcgcccat ccatttccag
     8521 agcacttcta cccgctccac gttgtgctcg gcgctgctgg tgaaaagtgg aaggccgagc
     8581 tgatccactc ctcctgggat catggcacac tttgccacgg ctcctacaag ttcacctccg
     8641 ccggatccgg agcaaccaac tttagcctgc tcaagcaagc aggagatgtt gaggaaaatc
     8701 ctggccccat gaccgccatc aagatgaaca ccaacggcga gggcgagaca cagcacatcc
     8761 tcatgatccc gttcatggcg cagggccacc tcaggccatt tctcgaactc gccatgttcc
     8821 tctacaagcg ctcccacgtg atcatcaccc tgctcacaac tccgctcaat gccggcttcc
     8881 tcaggcacct ccttcaccac cattcctact cctccagcgg catcaggatc gtcgagctgc
     8941 cattcaactc caccaaccac ggactcccac cgggcatcga gaacaccgat aagctcacac
     9001 tcccgctcgt ggtgtccctc ttccattcca ccatcagcct cgatccgcac ctccgcgatt
     9061 acatctccag gcatttcagc ccagccaggc caccactctg cgtgatccat gatgtgttcc
     9121 tcggctgggt tgaccaggtg gccaaggatg tgggctctac aggcgtggtg ttcacaacag
     9181 gcggcgctta tggcacatcc gcctacgtgt ccatctggaa cgatctcccg caccagaact
     9241 actccgacga ccaagagttc ccgctgccag gcttcccaga gaaccataag ttccgcaggt
     9301 cccagctcca tcggttcctc agatatgccg acggctccga cgattggtcc aagtatttcc
     9361 agccgcagct ccgccagtcc atgaagtctt ttggctggct ctgcaactcc gtggaagaga
     9421 tcgagacact cggcttctcc atcctccgca actacaccaa gctgccgatc tggggcatcg
     9481 gcccacttat tgcttcccca gtgcagcact cctcctccga caacaattca acaggcgccg
     9541 agttcgtgca gtggctcagc ctcaaagagc cggactccgt cctctacatc tccttcggct
     9601 cccagaacac gatcagcccg acgcagatga tggaactcgc tgctggcctt gagtcctccg
     9661 agaagccatt cctctgggtg atcagagccc cgttcggctt cgacatcaac gaagagatgc
     9721 gcccagagtg gctgccagag ggctttgagg aacgcatgaa ggtgaagaaa cagggcaagc
     9781 tcgtgtacaa gctcggcccg cagcttgaga tcctcaacca tgaatccatc ggcggctttc
     9841 tcacccactg cggatggaac agcatccttg agtctcttcg cgagggcgtt ccgatgcttg
     9901 gatggccact tgctgccgag caggcctaca acctcaagta cctcgaagat gagatgggcg
     9961 tcgcggttga gcttgctaga ggcctcgaag gcgagatctc caaagagaag gtcaagcgca
    10021 tcgtcgagat gatccttgag cgcaacgagg gctccaaagg ctgggagatg aagaatcgcg
    10081 ccgtggaaat gggcaaaaag ctcaaggacg ccgtgaacga ggaaaaagag ctgaagggct
    10141 cctccgtgaa ggcgatcgac gatttcctcg acgccgtcat gcaggccaaa cttgagccaa
    10201 gcctccagtg agctttggtt tggtggtgtt atgaaggttg ttgatgaatg aatttgtgta
    10261 tgtttgaatg tttgtgttgt gattgaaact ctggtttgat ggtttgaagt gaataattgt
    10321 tgaataaaaa aactttggtt ttctgtgaaa ttgattaagc catgttattt taattgttca
    10381 ttgtctttgt gttgtcaaat tgcgcatttg aggttaagtt tttggtaacg ttttgaaccc
    10441 aaccaaatat aatacaattc tttatctaat tatcggataa ttaaatatcc aattttcatc
    10501 aagttggata tgtccactta taaggcattt ataactataa tattttcaat tataaatata
    10561 aatatttgtc ggttgtaatt gcacacaaat tggataacag ttagatattt tattgaatca
    10621 aatctaacac aataatcgaa atagatatcc attagataaa actaaaccaa tttaatatcc
    10681 taattaaagg ttatttccct atataattca tgagttcgaa gtcatattaa tttgcagttt
    10741 tggtctttta catataattt ttgtaaatcc actattagtt acactttagt tttactagat
    10801 tatagcccgc gttcgct
//
