LOCUS       sequence-492677-        9469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of human codon optimized fusion of 
            hifiDn29-dCas9-P2A-GFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Fanton A, Bartie LJ, Martins JQ, Tran VQ, Goudy L, Kernick C, 
            Durrant MG, Wei J, Armour-Garb Z, Pawluk A, Konermann S, Marson A, 
            Gilbert LA, Roth TL, Hsu PD
  TITLE     Site-specific DNA insertion into the human genome with engineered 
            recombinases.
  JOURNAL   Nat Biotechnol. 2025 Nov 6. doi: 10.1038/s41587-025-02895-3.
  PUBMED    41199024
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Nov 6. doi: 10.1038/s41587-025-02895-3."
COMMENT     Sequence Label: pIVT_hifiDn29-dCas9-P2A-GFP
FEATURES             Location/Qualifiers
     source          1..9469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(join(1318..2031,2032..2034))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(1321..2031,2032..2034))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1363..1384)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1712..1731
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(2035..2088)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2104..2124)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(2128..3711,3712..3714,3715..6201,6202..6204,
                     6205..6228))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2128..6228)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6238..6258)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6385..6405)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     8236..8255
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8334..8438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8439..8507,8508..9299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8657..8676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccctag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtatc gagccccagc tggttctttc cgcctcagaa ggaagacgaa ttcgaagact
     1081 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tyyykktttt
     1141 ttttwwawww ymwwkkkkwa wrgrkrwwkk kkkkwwwwwt tttttttttt tttttttttt
     1201 tttttttttt tttttttttt ttttcttcct actcaggctt tattcaaaga ccaagaggta
     1261 caggtgcaag ggagagaaga agggcatggc cagaaggcaa gccccgcaga aggcagctta
     1321 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     1381 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
     1441 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     1501 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     1561 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     1621 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     1681 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     1741 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     1801 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     1861 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     1921 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     1981 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcactgggcc
     2041 aggattctcc tcgacgtcac cgcatgttag cagacttcct ctgccctctc cactgccgct
     2101 agccaccttc ctctttttct taggtccgtc gcctcccagc tgagacaggt cgatccgtgt
     2161 ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct ctttggtgct
     2221 ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag gggctcccag
     2281 attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg gcttatcccg
     2341 gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga tcactctctt
     2401 ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct gttccacaaa
     2461 cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt ggctggccag
     2521 gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct gcagttcgcc
     2581 ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt acttaggcag
     2641 cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccagaa agtcgatggg
     2701 attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt tcacactctt
     2761 cagtttcttg gacttgccct tttccacttt ggccaccacc agcacagaat aggccacggt
     2821 ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg cgatcagctt
     2881 atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct gcacctcggt
     2941 ctttttcacg atattcactt ggggcatgct cagcactttc cgcacggtgg caaaatcccg
     3001 gcccttatcc cacacgatct ccccggtttc gccgtttgtc tcgatcagag gccgcttccg
     3061 gatctcgccg ttggccaggg taatctcggt cttgaaaaag ttcatgatgt tgctgtagaa
     3121 gaagtacttg gcggtagcct tgccgatttc ctgctcgctc ttggcgatca tcttccgcac
     3181 gtcgtacacc ttgtagtcgc cgtacacgaa ctcgctttcc agcttagggt actttttgat
     3241 cagggcggtt cccacgacgg cgttcaggta ggcgtcgtgg gcgtggtggt agttgttgat
     3301 ctcgcgcact ttgtaaaact ggaaatcctt ccggaaatcg gacaccagct tggacttcag
     3361 ggtgatcact ttcacttccc ggatcagctt gtcattctcg tcgtacttag tgttcatccg
     3421 ggagtccagg atctgtgcca cgtgctttgt gatctgccgg gtttccacca gctgtctctt
     3481 gatgaagccg gccttatcca gttcgctcag gccgcctctc tcggccttgg tcagattgtc
     3541 gaactttctc tgggtaatca gcttggcgtt cagcagctgc cgccagtagt tcttcatctt
     3601 cttcacgacc tcttcggagg gcacgttgtc gctcttgccc cggttcttgt cgcttctggt
     3661 cagcaccttg ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga tagcgtccac
     3721 atcgtagtcg gacagccggt tgatgtccag ttcctggtcc acgtacatat cccgcccatt
     3781 ctgcaggtag tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc
     3841 tttcaggatc tggctgccca gctctttgat gccctcttcg atccgcttca ttctctcgcg
     3901 gctgttcttc tgtcccttct gggtggtctg gttctctctg gccatttcga tcacgatgtt
     3961 ctcgggcttg tgccggccca tcactttcac gagctcgtcc accaccttca ctgtctgcag
     4021 gatgcccttc ttaatggcgg ggctgccggc cagattggca atgtgctcgt gcaggctatc
     4081 gccctggccg gacacctggg ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg
     4141 gatcagctgc atgaagtttc tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt
     4201 cttgccggac tgcttgtccc ggatgccgtt gatcagcttc cggctcagcc tgccccagcc
     4261 ggtgtatctc cgccgcttca gctgcttcat cactttgtcg tcgaacaggt gggcataggt
     4321 tttcagccgt tcctcgatca tctctctgtc ctcaaacagt gtcagggtca gcacgatatc
     4381 ttccagaatg tcctcgtttt cctcattgtc caggaagtcc ttgtccttga taattttcag
     4441 cagatcgtgg tatgtgccca gggaggcgtt gaaccgatct tccacgccgg agatttccac
     4501 ggagtcgaag cactcgattt tcttgaagta gtcctctttc agctgcttca cggtcacttt
     4561 ccggttggtc ttgaacagca ggtccacgat ggcctttttc tgctcgccgc tcaggaaggc
     4621 gggctttctc attccctcgg tcacgtattt cactttggtc agctcgttat acacggtgaa
     4681 gtactcgtac agcaggctgt gcttgggcag caccttctcg ttgggcaggt tcttatcgaa
     4741 gttggtcatc cgctcgatga agctctgggc ggaagcgccc ttgtccacca cttcctcgaa
     4801 gttccagggg gtgatggttt cctcgctctt tctggtcatc caggcgaatc tgctgtttcc
     4861 cctggccaga gggcccacgt agtaggggat gcggaaggtc aggatcttct cgatcttttc
     4921 ccggttgtcc ttcaggaatg ggtaaaaatc ttcctgccgc cgcagaatgg cgtgcagctc
     4981 tcccaggtgg atctggtggg ggatgctgcc gttgtcgaag gtccgctgct tccgcagcag
     5041 gtcctctctg ttcagcttca cgagcagttc ctcggtgccg tccatctttt ccaggatggg
     5101 cttgatgaac ttgtagaact cttcctggct ggctccgccg tcaatgtagc cggcgtagcc
     5161 gttcttgctc tggtcgaaga aaatctcttt gtacttctca ggcagctgct gccgcacgag
     5221 agctttcagc agggtcaggt cctggtggtg ctcgtcgtat ctcttgatca tagaggcgct
     5281 caggggggcc ttggtgatct cggtgttcac tctcaggatg tcgctcagca ggatggcgtc
     5341 ggacaggttc ttggcggcca gaaacaggtc ggcgtactgg tcgccgatct gggccagcag
     5401 gttgtccagg tcgtcgtcgt aggtgtcctt gctcagctgc agtttggcat cctcggccag
     5461 gtcgaagttg ctcttgaagt tgggggtcag gcccaggctc agggcaatca ggttgccgaa
     5521 caggccattc ttcttctcgc cgggcagctg ggcgatcaga ttttccagcc gtctgctctt
     5581 gctcagtctg gcagacagga tggccttggc gtccacgccg ctggcgttga tggggttttc
     5641 ctcgaacagc tggttgtagg tctgcaccag ctggatgaac agcttgtcca cgtcgctgtt
     5701 gtcggggttc aggtcgccct cgatcaggaa gtggccccgg aacttgatca tgtgggccag
     5761 ggccagatag atcagccgca ggtcggcctt gtcggtgctg tccaccagtt tctttctcag
     5821 gtggtagatg gtggggtact tctcgtggta ggccacctcg tccacgatgt tgccgaagat
     5881 ggggtgccgc tcgtgcttct tatcctcttc caccaggaag gactcttcca gtctgtggaa
     5941 gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat agcagatccg
     6001 gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg cctcggctgt
     6061 ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt gccggtcggt
     6121 gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga tcacggccca
     6181 gcccacagag ttggtgccga tggccaggcc gatgctgtac ttcttgtcgc tggcctccac
     6241 ctttctcttc ttcttggggc tgctgctgcc gccgctgctg ccgccacttg attccggtgt
     6301 cgctgattca gatgtacccg gagtctcact cccactgctt tctggcgtgg cgctttcact
     6361 ggtaccgggt gtctcgctac cagacacctt cctctttttc ttaggtccgc tgttgatctg
     6421 aggcagctta ggataaagca ccaaggtgaa gtcatcgttt ctctggtgct tttcttttct
     6481 gtaaacagcg tagtccagca cgctcttcag caggttgttc ttcttggcag ggtcctgggt
     6541 cttgtagtac agttcaagca cgttctcgac tgtggggatg atatccttct tcactttgtt
     6601 ccgctgcacc tcctcggcca gagccttctc ggcgtcgtgg atggccttct tggtatcatc
     6661 gatgcgttca gccaggatgt tgcttctttc caggaatgta tcgatgctgt acacgccctt
     6721 ctccagcaga ttgtgcaggt tgtccttctg cttgtacagg tcattcagtt ccttctccag
     6781 ccggtggggg gctttctgtc tcaggtccac gctgtcgtcg ttcaccttgg gtctctgatg
     6841 tttgaaccaa tcggccttgt actgtgccag cagctggtgc aggccttgga taattttgct
     6901 ttccacgtac tcgaatctgc tagacttgtt ggtacagtat cttttgtagc agatcaggtg
     6961 ggggtggcgc ttgtgggtgt aaggtctata caccatgctg ctaccacaaa tcccgcactt
     7021 gaccagtccg gccagggggt tagtgatgcc gttctgcttc tgatagggca cgtggtactt
     7081 ccgctccagg atctcttggg ccttcaggta ggtttccatg gacaccagag gttcgtgctt
     7141 gccctccacg tcgatccatt gttccttggg ccgcatccgg acatcctttt ttttgcctgg
     7201 tgtgatagat tttttttcct cccgcttccg ccactggatt ctgccggcgt acacggcatt
     7261 cttcaactta gacaacacgg tggagacctt ccaggggatg cctgtagggc tcttgcagaa
     7321 cttgttcagc tcgttggcga tcttgctggc gcccatccgc acgttaggat cgtcgtgggt
     7381 gtaccagtca aagatcatct tcacgatagg ggcctgctca gggtgaggaa tcaggtaccg
     7441 ctccttgttg tttttctcga tgatgtagcc gtaaggtggt cttggagcga tgtaatttcc
     7501 ctcctcaact gtggccactc tgccccgctg cagtcttctg gtgataaact tgaattcccg
     7561 cctggccatg aaggcgccaa actcggcgta ttcctcgtcg aactcgttgt tcaggtcgta
     7621 tgttttcaga ggggtgatga tcttggtgtt gctattcttg aaggcctcca gcacaatgcc
     7681 ctgctccttg gtattgcctc tgcccagtct gtcgatgtcc atgcacagca cagcgtcgta
     7741 cagtccctct tccaccccct tcagtgtctc cagcatctca ggtctgtgga tcagagactc
     7801 gccgctgatg cccacttcct ctctgatctt cacgatgttc agatttcttt ctttggccag
     7861 cttcagcagg atgtttctat gtttagccag ggtttcgccc tcgcccctgg cctcagcttc
     7921 cagatcggct ctggactttc taaggtgtat gagcacccgt tccattctta tatttcttct
     7981 tactcttctt ttctctctta tttccttata gtgtgtcgta ttagcggccg cggatctcta
     8041 gcggatctga cggttcacta aaccaacgcg tatatctggc ccgtacatcg cgaagcagcg
     8101 caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg
     8161 tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat
     8221 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtcgaccct aggggatcgg
     8281 gagatcgatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8401 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8461 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8521 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8581 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8641 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8701 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8761 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8821 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8881 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8941 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9001 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9061 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9121 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9181 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9241 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9301 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9361 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9421 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
