LOCUS       sequence-492748-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and
            UGI, Include Golden Gate compatible cassette for sgRNA insertion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Lin H-C, Hsiao W-C, Hsu Y-C, Lin M-C, Hsu C-C, Zhang MM
  TITLE     Highly efficient CRISPR-Cas9 base editing in Bifidobacterium with 
            bypass of restriction modification systems.
  JOURNAL   Appl Environ Microbiol. 2025 Mar 10:e0198524. doi: 
            10.1128/aem.01985-24.
  PUBMED    40062897
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2025 Mar 10:e0198524. doi: 10.1128/aem.01985-24."
COMMENT     Sequence Label: cBEST2
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..22
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             146..832
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(881..904,905..907,908..4981)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4994..5245
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     terminator      complement(5426..5474)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     primer_bind     5797..5819
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5811..5828
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5812..5828
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5838..5857
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5838..5856
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5904..5922)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5943..5959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5943..5959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5956..5978)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5967..5983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5991..5997,5998..6015,6016..6021))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6036..6057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        6099..6174
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      6196..6230
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(6272..6860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6352..6371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgcaattaac cctcactaaa gggaacaaaa gctggagctc cagaattctg ttcacattcg
       61 aacggtctct gctttgacaa catgctgtgc ggtgttgtaa agtcgtggcc aggagaatac
      121 gacagcgtgc aggactgggg gagttatgtc ctccgagacc ggccccgtgg ccgtggaccc
      181 gaccctgcgt cgccgcatcg agccgcacga gttcgaggtg ttcttcgacc cgcgcgagct
      241 gcgcaaggag acctgcctgc tgtacgagat caactggggc ggccggcact ccatctggcg
      301 ccacacctcg cagaacacca acaagcacgt cgaggtcaac ttcatcgaga agttcaccac
      361 cgagcggtac ttctgcccga acacccgctg ctccatcacc tggttcctgt cctggtcccc
      421 ctgcggcgag tgctcccggg ccatcaccga gttcctgtcc cgctacccgc acgtcaccct
      481 gttcatctac atcgcccggc tgtaccacca cgccgacccg cgcaaccggc agggcctgcg
      541 cgacctgatc tcctccggcg tcaccatcca gatcatgacc gagcaggagt ccggctactg
      601 ctggcggaac ttcgtcaact actccccgtc caacgaggcc cactggcccc gctacccgca
      661 cctgtgggtc cgcctgtacg tcctggagct gtactgcatc atcctgggcc tgccgccctg
      721 cctgaacatc ctgcgtcgca agcagccgca gctgaccttc ttcaccatcg ccctccagtc
      781 ctgccactac cagcgcctgc cgccccacat cctgtgggcc accggcctga agtccggctc
      841 cgagaccccg ggcacctcgg agtccgccac cccggagtcc gacaagaagt actccatcgg
      901 cctcgcgatc ggcaccaact ccgtgggctg ggcggtcatc accgacgagt acaaggtccc
      961 ctccaagaag ttcaaggtcc tgggcaacac cgaccggcac tcgatcaaga agaacctgat
     1021 cggcgccctg ctcttcgaca gcggcgagac cgccgaggcg acccgcctga agcggaccgc
     1081 gcgtcgccgc tacacccggc gcaagaaccg catctgctac ctgcaggaaa tcttctccaa
     1141 cgagatggcc aaggtggacg actcgttctt ccaccgcctg gaggagagct tcctggtgga
     1201 ggaggacaag aagcacgagc gccacccgat cttcggcaac atcgtggacg aggtggccta
     1261 ccacgagaag taccccacca tctaccacct ccgcaagaag ctggtggact cgaccgacaa
     1321 ggcggacctg cggctcatct acctggccct cgcgcacatg atcaagttcc gcggccactt
     1381 cctcatcgag ggcgacctga acccggacaa ctccgacgtg gacaagctct tcatccagct
     1441 ggtgcagacc tacaaccagc tgttcgagga gaaccccatc aacgccagcg gcgtggacgc
     1501 caaggcgatc ctctccgcgc gcctgagcaa gtcccggcgc ctggagaacc tcatcgccca
     1561 gctgccgggc gagaagaaga acggcctctt cggcaacctg atcgcgctgt cgctcggcct
     1621 gacccccaac ttcaagagca acttcgacct ggccgaggac gcgaagctcc agctgtccaa
     1681 ggacacctac gacgacgacc tggacaacct gctcgcccag atcggcgacc agtacgcgga
     1741 cctcttcctg gccgcgaaga acctctcgga cgccatcctg ctcagcgaca tcctgcgggt
     1801 caacaccgag atcaccaagg ccccgctgtc ggcgagcatg atcaagcggt acgacgagca
     1861 ccaccaggac ctgaccctgc tcaaggccct cgtgcgccag cagctgcccg agaagtacaa
     1921 ggaaatcttc ttcgaccagt ccaagaacgg ctacgccggc tacatcgacg gcggcgcgtc
     1981 gcaggaggag ttctacaagt tcatcaagcc gatcctggag aagatggacg gcaccgagga
     2041 gctgctcgtc aagctgaacc gcgaggacct gctccgcaag cagcggacct tcgacaacgg
     2101 ctccatcccg caccagatcc acctgggcga gctccacgcc atcctccggc gccaggagga
     2161 cttctacccc ttcctgaagg acaaccgcga gaagatcgag aagatcctga ccttccgcat
     2221 cccgtactac gtcggccccc tggcccgcgg caactcccgg ttcgcgtgga tgacccggaa
     2281 gtcggaggag accatcaccc cgtggaactt cgaggaggtc gtggacaagg gcgcgtccgc
     2341 gcagtcgttc atcgagcgca tgaccaactt cgacaagaac ctcccgaacg agaaggtcct
     2401 gcccaagcac tccctgctct acgagtactt caccgtgtac aacgagctga ccaaggtcaa
     2461 gtacgtgacc gagggcatgc ggaagccggc cttcctgtcg ggcgagcaga agaaggcgat
     2521 cgtggacctg ctcttcaaga ccaaccgcaa ggtcaccgtg aagcagctga aggaggacta
     2581 cttcaagaag atcgagtgct tcgactccgt cgagatcagc ggcgtggagg accgcttcaa
     2641 cgcctccctg ggcacctacc acgacctgct caagatcatc aaggacaagg acttcctcga
     2701 caacgaggag aacgaggaca tcctggagga catcgtcctc accctgaccc tcttcgagga
     2761 ccgcgagatg atcgaggagc ggctcaagac ctacgcccac ctgttcgacg acaaggtgat
     2821 gaagcagctg aagcgtcgcc gctacaccgg ctggggccgc ctctcccgga agctgatcaa
     2881 cggcatccgg gacaagcaga gcggcaagac catcctggac ttcctcaagt ccgacggctt
     2941 cgccaaccgc aacttcatgc agctcatcca cgacgacagc ctgaccttca aggaggacat
     3001 ccagaaggcc caggtctcgg gccagggcga cagcctccac gagcacatcg ccaacctggc
     3061 gggctccccg gcgatcaaga agggcatcct ccagaccgtc aaggtcgtgg acgagctggt
     3121 caaggtgatg ggccgccaca agcccgagaa catcgtgatc gagatggccc gggagaacca
     3181 gaccacccag aagggccaga agaactcgcg cgagcggatg aagcggatcg aggagggcat
     3241 caaggagctc ggcagccaga tcctgaagga gcacccggtc gagaacaccc agctgcagaa
     3301 cgagaagctg tacctctact acctgcagaa cggccgcgac atgtacgtgg accaggagct
     3361 cgacatcaac cggctgtccg actacgacgt ggaccacatc gtgccgcagt ccttcctgaa
     3421 ggacgactcg atcgacaaca aggtcctgac ccgctcggac aagaaccggg gcaagtccga
     3481 caacgtgccc tcggaggagg tcgtgaagaa gatgaagaac tactggcgcc agctgctcaa
     3541 cgccaagctc atcacccagc gcaagttcga caacctgacc aaggccgagc ggggcggcct
     3601 gagcgagctc gacaaggcgg gcttcatcaa gcgccagctg gtcgagaccc ggcagatcac
     3661 caagcacgtg gcccagatcc tggactcccg gatgaacacc aagtacgacg agaacgacaa
     3721 gctgatccgc gaggtcaagg tgatcaccct caagagcaag ctggtctccg acttccgcaa
     3781 ggacttccag ttctacaagg tccgggagat caacaactac caccacgccc acgacgcgta
     3841 cctgaacgcc gtcgtgggca ccgcgctgat caagaagtac ccgaagctgg agtccgagtt
     3901 cgtctacggc gactacaagg tctacgacgt gcgcaagatg atcgccaaga gcgagcagga
     3961 gatcggcaag gccaccgcga agtacttctt ctactccaac atcatgaact tcttcaagac
     4021 cgagatcacc ctggccaacg gcgagatccg caagcggccc ctgatcgaga ccaacggcga
     4081 gaccggcgag atcgtctggg acaagggccg cgacttcgcc accgtccgga aggtgctgtc
     4141 gatgccgcag gtcaacatcg tgaagaaaac cgaggtgcag accggcggct tcagcaagga
     4201 gtccatcctc cccaagcgca acagcgacaa gctgatcgcc cggaagaagg actgggaccc
     4261 gaagaagtac ggcggcttcg acagccccac cgtcgcctac tccgtgctgg tcgtggcgaa
     4321 ggtcgagaag ggcaagagca agaagctgaa gtccgtgaag gagctgctcg gcatcaccat
     4381 catggagcgc tcctcgttcg agaagaaccc gatcgacttc ctggaggcca agggctacaa
     4441 ggaggtcaag aaggacctca tcatcaagct gcccaagtac agcctgttcg agctggagaa
     4501 cggccgcaag cggatgctcg cctccgcggg cgagctgcag aagggcaacg agctggccct
     4561 cccgtcgaag tacgtcaact tcctgtacct cgcgtcccac tacgagaagc tgaagggctc
     4621 gcccgaggac aacgagcaga agcagctctt cgtggagcag cacaagcact acctggacga
     4681 gatcatcgag cagatcagcg agttcagcaa gcgcgtcatc ctggccgacg cgaacctcga
     4741 caaggtgctg tccgcctaca acaagcaccg cgacaagccg atccgggagc aggcggagaa
     4801 catcatccac ctgttcaccc tcaccaacct gggcgccccc gccgcgttca agtacttcga
     4861 caccaccatc gaccgcaagc ggtacacctc caccaaggag gtcctcgacg cgaccctgat
     4921 ccaccagagc atcaccggcc tgtacgagac ccgcatcgac ctgtcccagc tcggcggcga
     4981 ctccggcggc tccaccaacc tgtccgacat catcgagaag gagaccggca agcagctggt
     5041 catccaggag tccatcctga tgctgccgga ggaagtcgag gaagtcatcg gcaacaagcc
     5101 cgagtccgac atcctggtcc acaccgccta cgacgagtcc accgacgaga acgtcatgct
     5161 gctgacctcg gacgcccccg agtacaagcc ctgggccctg gtcatccagg actccaacgg
     5221 cgagaacaag atcaagatgc tgtgatctag aggatccccg ggtaccgagc tcggattccc
     5281 cagatctaaa gttttgtcgt ctttccagac gttagtaaat gaattttctg tatgaggttt
     5341 tgctaaacaa ctttcaacag tttcagcgga gtgagaatag aaaggaacaa ctaaaggaat
     5401 tgcgaataat aattttttca cgttgaaaat ctccaaaaaa aaaggctcca aaaggagcct
     5461 ttaattgtat cggtttatca gcttgctttc gaggtgaatt tcttaaacag cttgataccg
     5521 atagttgcgc cgacaatgac aacaaccatc gcccacgcat aaccgatata ttcgactagt
     5581 tgttcacatt cgaacggtct ctgctttgac aacatgctgt gcggtgtgta aagtcgtggc
     5641 caaagtcttc tcagccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     5701 gggaaggggg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     5761 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     5821 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc
     5881 tcgaggtcct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     5941 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6001 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6061 gcgttgcgct cactgcccgc ttccaccggt gaagacatgt tttagagcta gaaatagcaa
     6121 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcttttta
     6181 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat ctagaggcca
     6241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gacccccccg
     6541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6601 acgacttatc gccgctggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6841 gcagaaaaaa aggatctcaa gcggccgcat cccattatgc tttggcagtt tattcttgac
     6901 atgtagtgag ggggctggta taatcacata cggccgataa agcaagcata taatattgcg
     6961 tttcatcttt agaagcgaat ttcgccaata ttataattat caaagagagg ggtggcaaac
     7021 ggtatttggc attattaggt taaaaaatgt agaaggagag tgaaacccat gaactttaat
     7081 aaaattgatt tagacaattg gaagagaaaa gagatattta atcattattt gaaccaacaa
     7141 acgactttta gtataaccac agaaattgat attagtgttt tataccgaaa cataaaacaa
     7201 gaaggatata aattttaccc tgcatttatt ttcttagtga caagggtgat aaactcaaat
     7261 acagctttta gaactggtta caatagcgac ggagagttag gttattggga taagttagag
     7321 ccactttata caatttttga tggtgtatct aaaacattct ctggtatttg gactcctgta
     7381 aagaatgact tcaaagagtt ttatgattta tacctttctg atgtagagaa atataatggt
     7441 tcggggaaat tgtttcccaa aacacctata cctgaaaatg ctttttctct ttctattatt
     7501 ccatggactt catttactgg gtttaactta aatatcaata ataatagtaa ttaccttcta
     7561 cccattatta cagcaggaaa attcattaat aaaggtaatt caatatattt accgctatct
     7621 ttacaggtac atcattctgt ttgtgatggt tatcatgcag gattgtttat gaactctatt
     7681 caggaattgt cagataggcc taatgactgg cttttataat atgagataat gccgactgta
     7741 ctttttacag tcggttttct aatgtcacta acctgccccg ttagttgatg catgatcatc
     7801 ataattctgt ctcattatct agagtcgcca ttggggaaga agagcaggat ttgcctgcgg
     7861 ttgaccgaga cggagtgggc gacgatgaac cggatggcgt tcgcgtgcgg tcagatggat
     7921 atctccatgg acacccacat ggatatctcc atggacaccc acatggatat ctccatggac
     7981 acccacatgg atatctccat caaatcgaag gtaagcaagc tagacggttc atgtacatga
     8041 accgaacgca gcacctccct cgccttgcgg cgctggagca acaacccgag tgccgaaggc
     8101 cgatggaaga ggaaaaagcc gcaaggcgag ggaggtgctg cgttcgcgtt tgggacaacg
     8161 tttagcgtca gtcgaacagt ggaatctcgc cgggtagatg cttcgatttc tccgccgcct
     8221 cgtcctcgcg ctccttgcga gccctgtagc agcgttgacg cacggcaccc tggctcatgt
     8281 tgaaatgctc ggcggtctgc ggccatgtat ggccctcgtc atcgtgatat gagcggactg
     8341 cttcgcgcat agcggcttgt tcatcaatcc aatcctcgcg cttcattgcc acccagctct
     8401 gaatggtgcg cgtggagacg ccgtatgctt cagctaattc acgtgcagac accgggcgct
     8461 tcttcctcaa agtcgtcctt accattccag tgcctccttc atgacctgct gatatttgtt
     8521 ctcaccgtgt ttgtgtcctc gtgcggattg gatggcgatg aatgtggctg cgttggcaat
     8581 ggcaccgtcg agccacatgc ggctgcgggt gacgatccat ttgtggatgc tcttggcgat
     8641 gtcctcgacc tcacgcgcgt gcagcggatc ggggaacagc gagacgttga gttcgtggca
     8701 ggtgcggcgc acgtataggc gcaatgcctc gctgctggcc tccgaacggt cgggcagccg
     8761 ccgcacgtag cggtaggcga gcgtgcgggc cttgtcgaag agcgtgcagt tgcgtcccag
     8821 cccctgcgtg cgggcgcgct tggtgcgctt ccagctgacg ggcggcatgt ccccgtgttc
     8881 ctggagcgac tgcacgagct gttccaggtc gtaggtgtcc cactcgatga tgtccgacgc
     8941 ccacgccgga tgctcggggt tcttcatcag aagtcccgaa tagcccatgt ccccgtcgca
     9001 ggagcggcgc agtccctccg tggtggcgtg caggagcttc aacggcttga gacgcgccag
     9061 atcggttctg ggcaccggaa aggtgagcac ccagcccgcg tgagcgtgcc cgttggccgg
     9121 gttctccgcg atccagttcg gccgcatgcc ctcgtgctcc cacagggcca tcgccttggc
     9181 gttcgcgtcg tcgatgtcca ccacgatcgt gttcaccagc gcggcggggt tcgcctcgat
     9241 gtgcttgcat ttgagcgcgt cggcgcggct cctgcggtgc agctgggagt agtcgtcgtc
     9301 gcagcacagc gggcgttcgg gcagccacca gccttcgaac gtgcgcgaca gctcaagcgt
     9361 cggctgcgaa tactcatcgg acatggctac agcatacatc gtatttgtac agtgaacaaa
     9421 tacgcgggaa ccaagtcaga tatttcagcc cagcgaagcg tcggctgaac ccagcccagc
     9481 acatcctgca cgaaaagccg ctggacggcc ctcagccgct tctccacgcc gctcacgtat
     9541 gcgcgagcct ctctgacctg ttcggccgcg tggaggccac tcacggacgt ttctgagccg
     9601 tctgagccgt cggcgtccgc tgcgcgttcc gcaggctgag gc
//
