LOCUS       sequence-492937-        2696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 expression vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMB1a2_Blue
FEATURES             Location/Qualifiers
     source          1..2696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..73
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        127..145
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     127..144
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    152..208
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(210..229)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(211..229)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(236..253)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(236..252)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(245..267)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        546..617
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(618..686,687..1478)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(836..855)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1649..2237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2138..2157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2391..2408
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2423..2563
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 cgtctcagtc aggagagaga cccctgttga caattaatca tcggcatagt atatcggcat
       61 agtataatac gacaaggtga ggaactaaac ccaggaggca gatcatgacc atgattacgc
      121 caagctattt aggtgacact atagaatact caagcttgca tgcctgcagg tcgactctag
      181 aggatccccg ggtaccgagc tcgaattcgc cctatagtga gtcgtattac aattcactgg
      241 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      301 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      361 cccaacagtt gcgcagcctg aatggcgaat gacgcctgat gcggtatttt ctccttacgc
      421 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
      481 catagggtct ctcgcttgag acgaagcttg acacggttat ccacagaatc aggggatact
      541 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      601 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      661 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      721 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      781 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      841 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
      901 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
      961 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1021 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1081 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1141 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1201 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1261 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1321 ccggtgagcg tggttctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1381 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1441 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1501 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1561 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1621 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1681 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1741 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1801 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1861 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1921 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1981 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2041 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2101 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2161 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2221 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2281 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2341 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2401 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2461 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     2521 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     2581 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     2641 gaccgtttta acgcgaattt taacaaaata ttaacgctta caattttaac aagctt
//
