LOCUS       sequence-492973-        9464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of NLS-tagged mOrange2 from EF1-alpha 
            promoter (for lentiviral packaging).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Van Eyndhoven LC, Kinsler G, Zhang J, O'Farrell A, Abderrahim R, 
            Srivastav A, Triandafillou CG, Greco TM, Zaret KS, Cristea IM, 
            Orzalli MH, Drayman N, Singh A, Raj A
  TITLE     Lineage memory shapes viral resistance barriers in human skin.
  JOURNAL   bioRxiv [Preprint]. 2025 Jun 27:2025.06.27.661951. doi: 
            10.1101/2025.06.27.661951.
  PUBMED    40666908
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Jun 27:2025.06.27.661951. doi: 
            10.1101/2025.06.27.661951."
COMMENT     Sequence Label: pCGT001_nuclear-mOrange2
FEATURES             Location/Qualifiers
     source          1..9464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      85..540
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(172..191)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     382..403
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        566..670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(671..739,740..1531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(889..908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1702..2290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2191..2210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2444..2461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2578..2599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2614..2619,2620..2637,2638..2644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2652..2668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2657..2679
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2676..2692
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2676..2692
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2711..2731
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2713..2731
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2759..2985
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2986..3166
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3213..3338
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3831..4064
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4249..4293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4524..4734
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4546..4742
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4593..4728
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4764..5783
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    5854..5971
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6109..7287
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6340..7278
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7235..7255
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7295..7304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7307..7327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7325..8029
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNYE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     KIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     CDS             8030..8050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    8075..8663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8128..8148)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8546..8557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8735..8968
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9046..9167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9083..9102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9137..9156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9207..9342
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9362..9381)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9363..9381)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9391..9408)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9391..9407)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9400..9422)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
       61 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      121 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      181 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      241 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      301 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      361 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      421 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
      481 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      541 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      601 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      661 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      721 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      781 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      841 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      901 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      961 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     1021 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1081 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1141 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1201 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     1261 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     1321 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     1381 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     1441 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1501 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1561 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1621 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1681 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1741 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1801 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1861 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1921 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1981 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2041 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2101 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2161 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2221 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2281 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2341 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2401 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2461 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2521 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2581 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2641 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2701 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2761 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2821 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2881 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2941 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     3001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     3181 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     3241 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     3301 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     3361 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     3421 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     3481 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3541 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3601 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3661 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3721 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3781 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3841 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3901 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3961 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     4021 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     4081 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     4141 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     4201 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     4261 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     4321 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     4381 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     4441 acccacctcc caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt
     4501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt
     4561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4741 aaaagctttc tagaggtacc accatgaaaa agcctgaact caccgctacc tctgtcgaga
     4801 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcc gagggcgaag
     4861 aatctcgggc tttcagcttc gatgtgggag ggcgtggata tgtcctgcgg gtgaatagct
     4921 gcgccgatgg tttctacaaa gatcgctatg tttatcggca ctttgcatcc gccgctctcc
     4981 ctattcccga agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc
     5041 gccgtgcaca gggtgtcacc ttgcaagacc tgcctgaaac cgaactgccc gctgttctcc
     5101 agcccgtcgc cgaggccatg gatgccatcg ctgccgccga tcttagccag accagcgggt
     5161 tcggcccatt cggacctcaa ggaatcggtc aatacactac atggcgcgat ttcatctgcg
     5221 ctattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcct
     5281 ccgtcgccca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     5341 acctcgtgca cgccgatttc ggctccaaca atgtcctgac cgacaatggc cgcataacag
     5401 ccgtcattga ctggagcgag gccatgttcg gggattccca atacgaggtc gccaacatct
     5461 tcttctggag gccctggttg gcttgtatgg agcagcagac ccgctacttc gagcggaggc
     5521 atcccgagct tgcaggatct cctcggctcc gggcttatat gctccgcatt ggtcttgacc
     5581 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggct cagggtcgct
     5641 gcgacgcaat cgtccggtcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     5701 gcgctgccgt ctggaccgat ggctgtgtgg aagtgctcgc cgatagtgga aacagacgcc
     5761 ccagcactcg tcctagggca aaggatctgc agtaatgagg cgcgccgtta acgaattcta
     5821 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     5881 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     5941 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     6001 actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta aacagagagg
     6061 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     6121 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     6181 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6241 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6301 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     6361 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
     6421 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     6481 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     6541 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     6601 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     6661 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     6721 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     6781 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     6841 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     6901 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     6961 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     7021 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     7081 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     7141 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     7201 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     7261 ttttttcttc catttcaggt gtcgtgaggc tagcgccacc atggtgccta agaaaaaacg
     7321 gaaagtgagc aagggcgagg agaataacat ggccatcatc aaggagttca tgcgcttcaa
     7381 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     7441 ccgcccctac gagggctttc agaccgctaa gctgaaggtg accaagggtg gccccctgcc
     7501 cttcgcctgg gacatcctgt cccctcattt cacctacggc tccaaggcct acgtgaagca
     7561 ccccgccgac atccccgact acttcaagct gtccttcccc gagggcttca agtgggagcg
     7621 cgtgatgaac tacgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     7681 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     7741 gatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     7801 tgccctgaag ggcaagatca agatgaggct gaagctgaag gacggcggcc actacacctc
     7861 cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt
     7921 cgacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     7981 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagc ctaagaaaaa
     8041 acggaaagta tgaggatcca cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     8101 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     8161 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     8221 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     8281 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8341 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8401 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8461 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     8521 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8581 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     8641 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     8701 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     8761 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     8821 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8881 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8941 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     9001 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     9061 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     9181 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9241 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9301 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     9361 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     9421 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catc
//
