LOCUS       sequence-493027-        8332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CRISPR-based technology to study chromosome-specific aneuploidy by
            targeting human centromeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Bosco N, Goldberg A, Zhao X, Mays JC, Cheng P, Johnson AF, Bianchi 
            JJ, Toscani C, Di Tommaso E, Katsnelson L, Annuar D, Mei S, 
            Faitelson RE, Pesselev IY, Mohamed KS, Mermerian A, 
            Camacho-Hernandez EM, Gionco CA, Manikas J, Tseng YS, Sun Z, Fani S,
            Keegan S, Lippman SM, Fenyo D, Giunta S, Santaguida S, Davoli T
  TITLE     KaryoCreate: A CRISPR-based technology to study chromosome-specific 
            aneuploidy by targeting human centromeres.
  JOURNAL   Cell. 2023 Apr 27;186(9):1985-2001.e19. doi: 
            10.1016/j.cell.2023.03.029. Epub 2023 Apr 18.
  PUBMED    37075754
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.03.029"; journalName: "Cell"; date: 
            "2023-04-27- 27"; volume: "186"; issue: "9"; pages: "1985-2001.e19"
COMMENT     Sequence Label: pLenti-gRNAX-2
FEATURES             Location/Qualifiers
     source          1..8332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    152..277
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             324..504
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(505..731)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(759..779)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(759..777)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(798..814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(798..814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(811..833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    822..838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(846..852,853..870,871..876))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    891..912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1029..1046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1200..1788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1280..1299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1959..2750,2751..2819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2582..2601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2820..2924)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2950..3405)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3087..3108)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3299..3318
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3532..3554
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3546..3563
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3547..3563
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3573..3592
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3573..3591
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3612..3747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3787..3908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3798..3817)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3852..3871
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3986..4219
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4291..4879
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4397..4408
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4806..4826
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4895..5494)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4978..4998)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5475..5494
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5507..6685)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5516..6454
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5539..5559)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6824..6941
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(7103..7351)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7161..7180)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7331..7351)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(7529..7573)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7758..7991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
       61 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
      121 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
      181 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
      241 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
      301 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
      361 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
      421 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
      481 gatctggtct aaccagagag acccgtttat gtatcgagct aggcacttaa atacaatatc
      541 tctgcaatgc ggcaattcag tggttcgtcc aatccatgtc agacccgtct gttgccttcc
      601 taataaggca cgatcgtacc accttacttc caccaatcgg catgcacggt gctttttctc
      661 tccttgtaag gcatgttgct aactcatcgt taccatgttg caagactaca agagtattgc
      721 ataagactac attaagcttg cagctccagc ttttgttccc tttagtgagg gttaattgcg
      781 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      841 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      901 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      961 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1021 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1081 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1141 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1201 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1261 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1321 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1381 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1441 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1501 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1561 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1621 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1681 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1741 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1801 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1861 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1921 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1981 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2041 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2101 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2161 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2221 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2281 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2341 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2401 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2461 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2521 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2581 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2641 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2701 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2761 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2821 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2881 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2941 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     3001 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     3061 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     3121 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     3181 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     3241 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     3301 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     3361 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     3421 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     3481 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     3541 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
     3601 gtacgtccct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     3661 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     3721 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggatag ctagagccag
     3781 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     3841 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     3901 aacaagttcc tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata
     3961 agatgacatg aactactata cgtactgcta gagattttcc acactgacta aaagggtctg
     4021 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     4081 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     4141 cagatctggt ctaaccagag agacccagta caagcaaaaa gcagatcttg tcttcgttgg
     4201 gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct
     4261 gccttgtaag tcattggtct taaagtcgac gcggggaggc ggcccaaagg gagatccgac
     4321 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
     4381 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
     4441 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
     4501 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
     4561 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
     4621 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
     4681 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
     4741 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     4801 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     4861 tgtaatccag aggttgattg tcgacttaac gcgttcaggc accgggcttg cgggtcatgc
     4921 accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct
     4981 cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct
     5041 cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg
     5101 gcgagactcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga
     5161 ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc
     5221 cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccacgg
     5281 cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga
     5341 gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg
     5401 cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg
     5461 tggcgaggcg caccgtgggc ttgtactcgg tcatggtggc cgtacgtcac gacacctgaa
     5521 atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact
     5581 caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct
     5641 ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg
     5701 acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg
     5761 agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac
     5821 ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa
     5881 gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg
     5941 gggcgataca cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc
     6001 cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg
     6061 acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt
     6121 ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat
     6181 tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc
     6241 gcacgcggcg gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc
     6301 cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa
     6361 tcacgtactg cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga
     6421 ggccaggccc gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc
     6481 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
     6541 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
     6601 caccggatca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
     6661 ctgcccactg acgggcaccg gagccaattc ccactccttt caagacctag aaggtccatt
     6721 agctgcaaag attcctctct gtttaaaact ttatccatct ttgcacccgg gccccctcga
     6781 gccggcgcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
     6841 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
     6901 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
     6961 tttgtctcaa gatctagtta cgccaagctt aaaaaagcac cgactcggtg ccactttttc
     7021 aagttgataa cggactagcc ttatttaaac ttgctatgct gtttccagca tagctcttaa
     7081 accaagggga tatttggacc tcggtgtttc gtcctttcca caagatatat aaagccaaga
     7141 aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta
     7201 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct
     7261 ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca
     7321 aatatgaagg aatcatggga aataggccct ctctgggtcc cctcggggtt gggaggtggg
     7381 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     7441 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     7501 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     7561 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     7621 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     7681 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     7741 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     7801 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     7861 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     7921 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     7981 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     8041 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     8101 aattgtccct catatctcct cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg
     8161 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     8221 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     8281 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gc
//
