LOCUS       sequence-49317-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, 
            Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, 
            Cheng L
  TITLE     Gene targeting of a disease-related gene in human induced 
            pluripotent stem and embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2009 Jul 2. 5(1):97-110.
  PUBMED    19540188
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Jul 2. 5(1):97-110."
COMMENT     Sequence Label: PIGAZFN-L1
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             155..742
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             758..799
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             809..826
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatctgtatg cgaaatttct cccagcggag cagcttggtg aggcatctac gtacgcacac
       61 cggcgagaag ccattccaat gccgaatatg catgcgcaac ttcagtgagc gcggcaacct
      121 gacccggcac ctaaaaaccc acctgagggg atcccaacta gtcaaaagtg aactggagga
      181 gaagaaatct gaacttcgtc ataaattgaa atatgtgcct catgaatata ttgaattaat
      241 tgaaattgcc agaaattcca ctcaggatag aattcttgaa atgaaggtaa tggaattttt
      301 tatgaaagtt tatggatata gaggtaaaca tttgggtgga tcaaggaaac cggacggagc
      361 aatttatact gtcggatctc ctattgatta cggtgtgatc gtggatacta aagcttatag
      421 cggaggttat aatctgccaa ttggccaagc agatgaaatg caacgatatg tcgaagaaaa
      481 tcaaacacga aacaaacata tcaaccctaa tgaatggtgg aaagtctatc catcttctgt
      541 aacggaattt aagtttttat ttgtgagtgg tcactttaaa ggaaactaca aagctcagct
      601 tacacgatta aatcatatca ctaattgtaa tggagctgtt cttagtgtag aagagctttt
      661 aattggtgga gaaatgatta aagccggcac attaacctta gaggaagtga gacggaaatt
      721 taataacggc gagataaact tttaagggcc cttcgaaggt aagcctatcc ctaaccctct
      781 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tac
//
