LOCUS       sequence-493373-        7829 bp    DNA     circular SYN 27-JAN-2026
DEFINITION  Expression of AEBP2LTEV (AEBP2 long isoform that can be converted to
            an AEBP2 short isoform by TEV cleavage) in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7829)
  TITLE     Davidovich Lab AEBP Plasmids 2025
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 27, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMB.AEBP2LTEV
FEATURES             Location/Qualifiers
     source          1..7829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        483..587
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(588..656,657..1448)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(806..825)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1619..2207
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2108..2127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2361..2378
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2510..2734
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2801..3334)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3523..3528,3529..3545,3546..3551))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        4099..4190
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4115..4132
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4242..5339
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      5196..5205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5313..5336
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5373..5396
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6072..6092
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    7131..7265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7168..7187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7222..7241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  7475..7640
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt cggggaaatg
      481 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      541 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      601 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      661 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      721 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      781 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      841 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      901 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      961 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1021 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1081 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1141 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1201 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1261 ctggctggtt tattgctgat aaatctggag ccggtgagcg tggttctcgc ggtatcattg
     1321 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1381 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1441 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1501 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1561 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1621 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1681 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1741 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1801 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1861 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1921 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1981 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2041 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2101 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2161 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2221 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2281 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2341 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     2401 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cagaccagcc gcgtaacctg
     2461 gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac
     2521 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     2581 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     2641 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     2701 gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca
     2761 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     2821 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     2881 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     2941 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     3001 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     3061 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     3121 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtcgc
     3181 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     3241 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     3301 cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat
     3361 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     3421 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     3481 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     3541 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     3601 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     3661 gactccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     3721 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     3781 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     3841 ttcgcccagg actctagcta tagttccctg aattcgcatc tagagcctgc actagacgaa
     3901 ctaggcaaga tgcgtccaat ccgtctaaac atggtgacaa cgctggacag atgacgtaac
     3961 accgagccac atcctgaaat cgaggcaggc taaccgaaac cgtgacaatg caaagagaca
     4021 gcctgaccgt ctcaggtaga ctacccatga gtcacaatga acgctagtgg ttggctacat
     4081 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
     4141 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
     4201 attcataccg tcccaccatc gggcgcggaa gaatgggtac caaaactgaa gaaggtaaac
     4261 tggtaatctg gattaacggc gataaaggct ataacggttt ggctgaagtc ggtaagaaat
     4321 tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat
     4381 tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct
     4441 ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg
     4501 acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc
     4561 cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tttgctgccg aacccgccaa
     4621 aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc
     4681 tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt
     4741 atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg
     4801 gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag
     4861 acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca
     4921 acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac
     4981 tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc gcaggtatta
     5041 acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat ctgctgactg
     5101 atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt
     5161 acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac gcccagaaag
     5221 gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg
     5281 tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagactg
     5341 ccgcaggcac cgattacgat atcccaacga ccctggaagt tctgttccag gggccctccg
     5401 gtatggccgc cgctatcacc gacatggccg acctggagga gctctcccgc ctgagccctc
     5461 tgccccccgg cagcccgggt tcggcggcgc ggggccgggc tgagcccccc gaggaggagg
     5521 aggaagagga ggaggaggaa gaggaggcgg aggccgaggc ggtggcggcg ctgctgctga
     5581 acggcggcag cggtgggggc ggcggaggcg gcggcggagg agtggggggc ggcgaggcag
     5641 acacgatgtc ggagccgagc cccgagagcg ccagccaggc cggggaggac gaagacgagg
     5701 aggaggacga cgaggaggag gaagatgaga gcagcagcag cggcgggggt gaggaggaga
     5761 gtagcgccga gagcctggtg ggcagcagcg gcgggagcag cagcgacgac acccgctcgt
     5821 tgagccccgg cgccgccagc agcagcagcg gggatgggga cggcaaggag ggcctggagg
     5881 agcccaaggg accgcggggc agccagggcg gcggcggggg cggcagcagt agcagcagcg
     5941 tagtctccag cggcggcgac gagggctacg ggactggggg aggcggaagc agcgcgacct
     6001 ccgggggccg gcggggcagc ttggagatgt cgtcggatgg ggaacccctg agccgcatgg
     6061 actcggagga cgaaaacttg tatttccagg gcatgtacac acgtcggtac tcaagcataa
     6121 gcagtactat aatggatgta gacagcacaa tttccagtgg gcgttcaact ccagcaatga
     6181 tgaatggaca aggaagcact acttcttcaa gcaaaaatat tgcctataat tgttgttggg
     6241 accagtgcca ggcttgcttc aactctagcc cagacctggc agatcacatc cgttccatac
     6301 atgtagatgg tcagcgagga ggggtatttg tttgcttatg gaaaggttgt aaagtatata
     6361 acactccatc taccagtcaa agttggttac aaaggcatat gctgacacac agtggagaca
     6421 aacctttcaa gtgtgttgtt ggtggctgca atgccagctt tgcttctcag ggagggctag
     6481 ctcgtcatgt acccacacac ttcagtcagc agaactcctc aaaagtttct agccagccaa
     6541 aggccaaaga agaatctcct tctaaagctg gaatgaacaa aaggaggaaa ttaaagaaca
     6601 aaagacgacg ctcattacca cggccacatg atttcttcga tgcacaaaca ctggatgcga
     6661 taagacatcg agccatatgc tttaacctct cagctcatat agaaagttta gggaagggac
     6721 acagtgttgt ttttcatagt actgtaatag ctaagagaaa agaagattct gggaagatca
     6781 aacttttgct tcattggatg cctgaagaca ttctgcctga tgtgtgggtg aatgaaagtg
     6841 aacgacatca gttaaaaact aaagtagttc atttatcaaa gctacccaaa gatactgcct
     6901 tgcttttgga cccaaacata tacagaacaa tgccgcagaa gaggttgaag agaactctga
     6961 taagaaaagt gttcaatttg tatttaagca aacagggatc ctaatgagtc gagaagtact
     7021 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     7081 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     7141 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7201 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7261 ggatctgatc actgatatcg cctaggagat ccggggctga agttgagacg acgcacaagg
     7321 tcagggcact catgcgacaa tcaactcgat gcatgatccg caccattgtc gaggggccag
     7381 cgtcaatagt gccgatgacc acagacccgg ttaagacata gccgaatgga gccgcgccga
     7441 ccacagaatg ataactagtg cactgcagta catcaaccag ataagtgaaa tctagttcca
     7501 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
     7561 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
     7621 aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat
     7681 cctgccaact ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga
     7741 aaatttttct gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat
     7801 ttgcagcctg aatggcgaat ggggggagt
//
