LOCUS       sequence-49372-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Hammond GR, Fischer MJ, Anderson KE, Holdich J, Koteci A, Balla T, 
            Irvine RF
  TITLE     PI4P and PI(4,5)P2 Are Essential But Independent Lipid Determinants 
            of Membrane Identity.
  JOURNAL   Science. 2012 Jun 21.
  PUBMED    22722250
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2012 Jun 21."
COMMENT     Sequence Label: LYN11-FRB-mcherry
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(118..144)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(151..429)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             444..461
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     promoter        complement(545..748)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(574..594)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
       61 ctccttgatg atggccatgt tatcctcctc gcccttgctc accatggtgg cgaccggtgc
      121 gtagtctggt acgtcgtacg gataactagt ctttgagatt cgtcggaaca catgataata
      181 gaggtcccag gcttgggtga ggtccttgac attccctgat ttcatgtact tcctgcacca
      241 ctcttgggcc tccattaaat ctcgaccata ggcctgatta aaggatgttt ccttcagagt
      301 ctgggggccc cgttccatca tagcatgcaa gggctccagc acctcaaaca tgcctttcac
      361 gttcctttcc ccaaagtaca aacgagatgc ctcttccagg ccttcatgcc acatctcatg
      421 ccagaggatt ctagatagga tagcaccagc accagcacca gcacctgaat tcgcagatct
      481 gctgtctttc ccttttgatt ttatacatcc catggtgcta gcggatctga cggttcacta
      541 aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      601 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
      661 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
      721 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
      781 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
      841 taccgtcatt gacgtcaata gggg
//
