LOCUS       sequence-493817-        6712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PopJ under the control of the PodJ promoter (KanR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Lu N, Duvall SW, Zhao G, Kowallis KA, Zhang C, Tan W, Sun J, 
            Petitjean HN, Tomares DT, Zhao GP, Childers WS, Zhao W
  TITLE     Scaffold-Scaffold Interaction Facilitates Cell Polarity Development 
            in Caulobacter crescentus.
  JOURNAL   mBio. 2023 Apr 25;14(2):e0321822. doi: 10.1128/mbio.03218-22. Epub 
            2023 Mar 27.
  PUBMED    36971555
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2023-04-25"; volume: "14(2):e0321822. doi"; pages: " 
            10.1128/mbio.03218-22. Epub 2023 Mar 27"
COMMENT     Sequence Label: pLN051
FEATURES             Location/Qualifiers
     source          1..6712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            21..130
                     /note="incP origin of transfer"
     primer_bind     282..298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      299..345
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1118..1135
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     primer_bind     complement(4536..4552)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4563..4579)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4572..4594)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4777..5365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4857..4876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5775..6590)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5775..6590)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6498..6517
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6582..6601
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ccaccgcggt ggcggccgcc ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
       61 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
      121 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
      181 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
      241 gaagcagggt tatgcagcgg aaaagatccg tcgacggccg ctctagaact agtggatcca
      301 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca
      361 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
      421 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtttttat ataagcttag
      481 gtgcggcgcc gaccatgatg gacgagctga agtccatctc ggactcctgg ctttcgcatc
      541 acgccggcgg cgaaaagagc ttctcgatgg ggggctttga cccacgttat gtggccgagt
      601 tccccgtcgc ggttgttcgc agtgaaggca agatcgtcgc cttcgccacg ctgtggacga
      661 cggccgccaa gacggcgttc tccatggacc tgatgcgcta ctctgacgaa gcgcccaaga
      721 acgtcatgga ctatctgttc gtcgagctgc tgcagtgggg caaggacgag ggatacagcg
      781 ccttcgagtt cggcgtcgcg ccgctggcgg gtctgcagga tcgccctctg gccccgatca
      841 tgtcgcgcgt cggccggctg ctctacgagc gcggcgagga gatctataac ttccagggtg
      901 tgcgtcgcta caaggacaag tacgacccgg tctggcagcc gcgctacatt gccgcgcctc
      961 agaagtgggc gatcccgttc ctgctcgccg acatcgggct gctgtcgtcc ggtggggtct
     1021 ccggcctggc gaaacgaccc aagaagctcc cggagaaggc cgcgacctag tagcgcgtct
     1081 ggttacccgt ctggacgccg agcaggttca ccagatgcac cagcatcagc atcagtccga
     1141 ccacgcagat catcatcatc ggccgccaag ggatcagccg cgggcccttg agaatattga
     1201 tcggacgcgc gcccagccag ccgaagagca cggcgaggat caggaacagg atcgcggcgg
     1261 cggcggtgac gggcaggtcc atgcgcgctt ctaggcctgc gacaatccgc ctggcaaatg
     1321 gttaaccagt gttaaccctc ttgcttttga accgcgctac agagtgatgg gactctgtcc
     1381 tgattcctcc acagtgaatc gcacagccac tacatctggc gttttcgtcg cgtttacacc
     1441 ctaggaatcg gtgactagcg ttttccccag gtgcggggag atcgattcgc atgacggcgg
     1501 cttcgccatg gagcgttaag gggattgacc ccaaggcacg ggaggtcgcg aaagacctcg
     1561 cgcgtcgctc cggcatgact ttgggtgaat ggctgaaccg aatgatcatc gaaggcgatg
     1621 gtcagaccgc tgatccgcgt ctcgcggggg acgatgtccc caaccgcgcc tatctcgaga
     1681 ttgtcaaaga tgatgcgccg ccgcgcatcg agatcgccga gcatccggca gatgaggttg
     1741 gtcgcgtcgc gctggcgctg gatcgcctga cccaacgcat tgaagccgcc gaggggcgca
     1801 acgccgccgc catcaccggc atcgatcact ccgtccgtga cgccctgacg cgtctgggcg
     1861 catccgaacg tgagcagatc gccgtcgccg cccggttcga gggcgctgtc gacgaactga
     1921 agaccgagca ggctcgggcc accgaacgtc tgcgccggat cgagagcgag gcggcgggtc
     1981 cgcgctcggc cgaggcgttg cgcgcgcttg aaggcgcctt gggcaaggtc gccggacacc
     2041 tttacgaagg cgaggcgcgg acgcgcgagg cgatcgcgac cctggaagcc aagctgaacc
     2101 agcagagctc gggcgatccc agcgcgctgg tcgaggctgt ggtcgcgcga ctgggcgaac
     2161 gcctcgaagc cgccgaaacc cgcaccagcg acgcgctgcg cgaactgggc gcgtcgttcc
     2221 aggccctgga tcagcgcttg ggcgccgtcg agactgccaa tcccgccacg ggcgttcagg
     2281 agggcctgga cagcctggcc gccaccctca cgcagaagat ggaagccgct cgcctggaga
     2341 tggcggccaa gcttcgcgaa agcgccgacg gacgtttcga tcgcatggag cgcaagctcg
     2401 gcgagatggc ggcccacgtg caggcggccg aacagcgctc ggcccaggcc attgagcgta
     2461 tgggccgcga gattgtcggt gtcgctgatg cgttcaatcg ccgcgtccac gccgccgaga
     2521 gccgtaacgc cagcgctatc gagcaggtcg gtggcgaggt tgcgcggatc gccgccagcg
     2581 tcgagcacaa gctcaatcgc gccgatagcg ttcaagccca ggcccttgaa aagctgggcg
     2641 gcgagatcgc ccggatcacc gagaagctgg ccgagcggat cggcagcgcc gagcgacgca
     2701 acgccctggc catcgacgat gtcggcgaac aggtcgcccg cgtcacagag cgcctgaacc
     2761 aacgccacga acgttccagc caggaactgg tcgaccgcat ccgccagagc gaagagcgca
     2821 cgctccgcat gctggaagaa gcgcgcgaga agatcgacag ccgtctgtcc gaggcgcagc
     2881 ggaagctgga agctgcgccg ccgtcgccgc cgcccgcgca ggctccggcg ccggttgcga
     2941 ctgctcagcg tccggttccg ccggcggctt cgcccttcga ggacaactat ttcagccagg
     3001 ccgcctcgtt cagcacgtct gaggatgagg ctgacgcgtt tgatgcgccg cctgcgccgg
     3061 cccgtagctt cgaggtggcg gagttcccgg ctgcggagcc ggaagaaccg gccttcgcac
     3121 acgatgacta cgcgatcgcc gatggcttcg agccggaaag cccgcgctat gaagtcgagc
     3181 ctgaggtctc cgacttcgcg cccgccgagc cgtcgcgtcc gatgtcgacc cgcgatatca
     3241 tcgagcaggc ccgcgccgcc gcgcgcgccg ccgccgcctc ggaaggcaag ggcggcaagg
     3301 ccaagtcggc gaagaaggaa aaggcttcca aggccagcgg ctcgctgttc agcggctttg
     3361 gcggtttctc gaccaagaag tcgaaggcgc gcttgggcgc gaccgtgacg acggccctcg
     3421 ttgtcttcgc cgccgccggc gcgctcgggg ccggcgtggg cggcctgctg ctgctgaaca
     3481 ccgacgacgg caacaactcg cccagccgcg tcgcccaggc gatcgctggt cgcaaggccg
     3541 atgtcgaggt gaatggtccc gaggctgaca cgacccccgg cgcgccgcgc gccgccgtcg
     3601 cgctgacgac gggcaaggtc gtcccagccg aggtcgaggc ccctgcagcc ccgccgacca
     3661 acgaagccaa ggccctgttc gaggacgcgg tccgtaagat cgagtcgggg gaccgctcgg
     3721 gcgtcgagtt gctgaagcgc gccgccaatg gcggctaccc ggccgcgcag ttctacctct
     3781 cgaagatgta cgagggcggc aagaacggcg tgaaggtcga tatggccgaa gcgcgccgct
     3841 ggagtgagcg cgccgccaac ggtggcgatc cgcgggccat gcacaacctg gccctctact
     3901 acttcaaggg cgagggcggt ccgcgcaatt cgacgacggc cgccagctgg ttccgcaagg
     3961 ccgccgacat gggcctggtc gacagccagt tcaacctggc tcagctctac gaaagcggac
     4021 tgggcgtcag ccagaatccg gccgaagcct acaaatggta tgtgatcgcc ggccgcgctg
     4081 gggattcgac cgcgcggggc cgcgccacgg cgctgcgctc gcagctgacc gcagaggccc
     4141 agcagaccgc tgaccgctcg gccttggcct tccgtccgca gactcaggtc cagaccgcca
     4201 gtctgtcgtc cgctgcgccg gccgccgcaa acgccaacct tggcgttgcg cagcgtgtcc
     4261 tgtcgcagct gggctattac caggggccgc gcgacggtgt ctcgtcgccc gccctgcgca
     4321 tggcgatcgc ggcctatcag cgtgatcagg gtctgccccc caccggatcg gtggacgccg
     4381 agaccctcaa tcgcctgtcg gtctacgcgc gctaaacgcc tcgcgacctc gcgctttgcc
     4441 gggaaggggc cttaattaat atgcatggta ccttaagatc tcgagctccg gagaattcga
     4501 acgttacgcg taccggtgct agctgcagcc cgggggatcc actagttcta gagcggccat
     4561 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4621 cgccttgcag cacatccccc tttcgccagc ggtatcagct cactcaaagg cggtaatacg
     4681 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4741 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4801 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4861 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4921 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4981 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5041 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5101 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5161 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5221 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5401 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5461 cacctagatc cttttagggg ggggggggcg ctgaggtctg cctcgtgaag aaggtgttgc
     5521 tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt
     5581 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga
     5641 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt
     5701 tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa
     5761 ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     5821 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     5881 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     5941 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     6001 ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact
     6061 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     6121 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     6181 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     6241 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     6301 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     6361 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     6421 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     6481 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     6541 gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     6601 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     6661 gcaatgtaac atcagagatt ttgagacaca acgtggcttt cccccccccc cc
//
