LOCUS       sequence-494279-       21299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 (+)-Eddh in Gordonibacter 
            urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21299)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 21299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF2-Elen0501-(+)-Eddh-Accs(ElA2)
FEATURES             Location/Qualifiers
     source          1..21299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1503..1514)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      7928..7955
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      15082..15109
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(15541..16392)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(16379..17218)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(17729..18700)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19939..20026
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      20368..20762
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20925..20968)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            21064..21173
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctat
     1501 tcgcgtcctt cgatcacgtc gaggaactcc tgcttgccgt ggacgtccat cttttggtag
     1561 atgtggcgca ggtgggtggt caccgtgccg cgcgacaggt agaggtcttc ctggatgcgg
     1621 gtgctcgagc ggcccttcgc gaacaggatc atgatctcgg tttccttcgg cgacagaccg
     1681 tattccccgg ccacgtcttt gcagcggtct tggaaacgct ggcgcctcgg ctcggcgccg
     1741 cccttgtcct catcgccctt cgccagcgcg atgaggtcgt tctcgttgaa cacgaacatg
     1801 tacgagatga ggatggccag cgtggccagc agcgcgatca ggctgaggct ctgcggcgag
     1861 aacggcgcga acgtgcacac gaactgcccc agcgaggaac cgagcagcac gccgagcgtc
     1921 accatgcccc agccgatgcc gaacaccatg acgcccgaca gccggtacgt gaacgagatg
     1981 tcggcgagca gtatccagat gagcacgttg aacgtgccgt agccggccag cgcgatggtg
     2041 ctctcgatct cggaaaagcc ggtgaacacg ggcagcagca tgaagaacac ggccatgacc
     2101 agcatcaccg agcggtacac gggacgtagg cccgttttgc tggagagcag cacgcagccg
     2161 tagacgatgg cgatggtgag caggctcgac caaaggagcg ggaagcggta gaagttctcg
     2221 acgctggcct cgttcgcggt gatgaacacc gcgcgcacca cgccgtcggc catgccgatg
     2281 aggcaggcgc acacgccgat cttccacgag aacttcccca ggtggatctc gaacggctgc
     2341 acgaccggct cggctttcga cgaccacgtc ctcagcttca cgaacagaat ccaacctgac
     2401 gcggcgggca gcagcgcgca gaccacgcac gctccgacgg gcggcaaggt cagaacggcg
     2461 gggaagaggg ccgcggcgat caggaaggcg agcggcgctt cgaacgacgt gcggcgggga
     2521 tcgacattgc ggtacagctc gccgtacccc aggtcgatga gccccgatcc ggcgccggtc
     2581 gataccgagc cgaacgcgca cagcgccagc gccggtgcgc tcggcagggt ggcgcaggcg
     2641 agggccagcg tgccgacggt ggtgagggcc gggccgagga agcggaacgc gcgatggccg
     2701 atgagcgccg tgaagcgccg gtggaagatc gcgcttgcga acagggtcgc gcacagcacg
     2761 agcgtcgatc ccaagtaagc cgcgccgggc agcggctcgc cgttccacgt gatggagctg
     2821 gtggcggcgc ctaggaacat gaggtacacc cacgcgcgcg ccagggcgaa gccgagcatg
     2881 cgcaggttca acaggctgcg cggcgccgtc tcgcgcaggc gatcccgggc ggtggtcagc
     2941 ggtttcggca tgtgcgtcac gagaattccc cctcaagatc tcgatcccct gagcctcatt
     3001 atagcgtgcg cgatccgtcc ggctcacgtg gcccgatagg ttgatgcgtg acgtcatgct
     3061 ttgctcatca ggtgtttctc caaaatcatg ggaaatgcat gatgggaaag cctccgattc
     3121 gcccgatttt ttgcagaaac gcatgatgcg cggacgtccc ctgccgcgca tgctcgaaca
     3181 aagcggcggg cgttctcgac gagccgtcgc aaggacgaga cggagaagga ggaggaatgg
     3241 gaaagctcaa tctgacgcgc cgtgcgttca cgaagctcac ggccgtgacg ggtgcggcgt
     3301 tggcctgcgc ggcagcggtc gccccgaacg cggcgctggc cgaagatgcc ggagcggccg
     3361 cgcgcggcga cgacgtgaaa cgcgtgcgca cgtgctgccg cggctgcgga aagatggagt
     3421 gcggcgtgtg ggtgaccgtg caaaacggcc gcgctatcaa ggtggagggc gaccagtcgt
     3481 cattccagtc gagcggcaac tgctgcggca agtcgcagtc gtccatccag gcggcgtacc
     3541 accccgaccg catctaccat cccatgaagc gcacgaatcc caagggcgag gatcccggct
     3601 ggcagcgcat cacgtgggac gaggcgatgg agatcgtcgg caccaagttc aacgagctga
     3661 tggaccgcta cggcggacag tgcatcttca acatggcggg cacgtcgcgc cagtgggtgt
     3721 acgggcccta cgcgttctac aagtggctgt tcgacacgcc gaacgcgcac gtggcgtcgg
     3781 aaatctgcaa ggggccgcgc cgtttgatgg ggtggatcag ctcggtcgac ggcgcgccgt
     3841 ggatggcgct gcgcgacggg ccgcgcgtgt acgtgcagtg ggggaccgcg cccgaaaact
     3901 cgaactacga tgacagctgc cgcaacctcg tggacaagat gaccgaggcc gacgtgcaca
     3961 tctgcatcga tccgcgcctg tccggctcgg gcaaggaggc cgactactgg ctgaacctgc
     4021 gtcccggctc cgacggcgcg ctggcgctgt gctggcagca cctcgtcatc aagaacgacc
     4081 tggtggactg ggagttcgtg aagcgctgga cgaactcgtc gttcctcgtg gtggaggacc
     4141 gggagcccac gggcggccgc tacatcgacc tgtccacgcc gctcaacaac gccggcatcc
     4201 ccgccgacgt cgtgggcacg aagctcaaga cgcgcctgct gaaggaaagc gatgtggtgg
     4261 agggcggcag cccgcgcaag ttctacgcgt ggaacaaact ggccaacgac ggcgccggcg
     4321 gcttggtgat gtgggacgtc gacaccacgc aatgggaggg ctgcaaccac gtggccccca
     4381 cgcgcgacca gatggaggtc gtgtacaagg gcacgtcgca ggagggctat ttgccgccgc
     4441 tgtcctatca cgagctggag gaagccggca tcgacctgga tatgcgtggg acgcatgagg
     4501 tggagctgct cgacggttcg aagcacacgg cgaagccggt gtgggcgtac ctcgaggaat
     4561 cggtggcgga ctgcacgccc gcgtggtgct ccgagatcac gggcctcgat cctgcgctca
     4621 tcgaagaggc gtgcctcgtg tgggccacgc gtcccgaggg gcaggattac ggcaacggcg
     4681 gtatccacct gaacctcgcg cccgaccaga tcggcaactg cacgcagacg gtgcgcgcgg
     4741 tgctgcacct catctacatg accggcaact tcgacacgcc cgccggcaac cgcggcctca
     4801 cgcgctcgcc catcgacgag caggccacgg ccgcgcccgg ctcgaacatg ccgcaggaag
     4861 tgaaggccca gctgatcgcc ttgggcgaga tccccgtgga aggcgtcacg cccgatcccc
     4921 tcaacgtgcc cgaccgctac gacacgctgt cgaacatggt gggcgccgac gagttcccca
     4981 tcacggcgta ctacaacgag tgggccgacg cgacgcgcat ctgggacgcc tgcctcaccg
     5041 gcgagccgta ccccgtgcgc ggcggcatca acgagtccgg ctcgttcatg aacatgtcga
     5101 acgcgaacct ggcctgggag gcgttgcagt cgctcgattt ctgggtggac atcaacatgt
     5161 tccaccatcc cggcaccgag atggccgaca tcctgctgcc gtgccagcat tggctggaga
     5221 tcaacaacat ccgcgtgtcg cagggcgcgt ccggcggtat cggcgccacc atccgcgcgg
     5281 tcgagccgcc cagcgacacg aagttcgact acgacatcaa ccgcctgctg ttcgacgccg
     5341 tgggcggccc gaacggaacc tggaccaaca tcgcgggcga cgcgcccggc ggctaccacg
     5401 tggacgagcg cttggaggac tggttccaga acaactcgaa gaccaatccc aaggtgaaat
     5461 ggcagcattg ggacgacttc gtggaggact ttcaggagaa cggctggatc aacgccaagg
     5521 agatcgagcc cgaccgctgg ggcacgtacc gccgcttcga gaccggctgg atgcgcatcg
     5581 gcaaggacgc gtgcaccggc tccacgttca gcgcggcgtt cgacgacgcc ggcaatccgg
     5641 tgaacaactt cggctgcccc acgccgacgg gcctcgtgga attttggccg ctcgtgttcg
     5701 agacgtactg cgtggacaag gcgaacgagt tcaaccccgg caagttcgac ctggtgcacg
     5761 agatgatgcc gcactacgac gagccgaaat cgggccccaa gggcgacgtg gacatgaacg
     5821 agtatcccat tatcctcacc accggccgcc gcatacccgt gtacttccat tccgagcacc
     5881 ggcagctgcc gtggtgtcgc gagctgtggc cggcaccgcg tttggaggtg aaccccgaag
     5941 acgctgccga gctggggctc gagcagggcg attgggcctg gatcgagacg gagtggggca
     6001 aggtgcgcca gtgcgtcgat ttgtactacg gcatcgcgaa ggggtgggcg aacgccgagc
     6061 acgcctggtg gttccccgag ctgcccgcgc cgacgcacgg gttcgagctg tcgaacatcg
     6121 agtgcatctg gaacccctac ggccaggatc cgttcatcgg gtcgtcccac atgcgcggcg
     6181 tgcccgtgaa gatatacaag gccacgcccg agaactgccc cgacggcaag gtcatcccct
     6241 gcgcgcccga ggacggcacc gagatcatct acgatgcctc cgacccgcgc ctgaaggaat
     6301 ggctgccgaa ctacaccatc cgagaggagg cgtaaagcca tgacccagta cgcgatcgtc
     6361 accgatttga accggtgcgt cggctgcctg gcctgcatgg ttgcctgcaa ggcggtcaac
     6421 aacgtgccgg tgggcaacta ttggaacaag gtgctgcgca tcggcccctc gctgaaagag
     6481 ggggccacgt cgtcgcacga cgtggagatg tactacctgc ccgtgcagtg ccagcactgc
     6541 gccgaccccg agtgcgtgaa ggtgtgcccg acgggcgcct cgcacaaact ggaggacggc
     6601 acggtgcaga tcgacaaggc gaagtgcatc ggctgccagt tctgcgcgat gtcgtgcccc
     6661 tacaacgtgc ggtacctgaa cgaggaagag ggcgtcgtgg agaagtgcac gctgtgcgcg
     6721 cagatcgtgg ggcagggcgg gttgccgcaa tgcgtcatcc agtgcggagg gcgcgcccgg
     6781 ttcttcggcg atctggacca ggggatcgag tcgttcgaag cccccgccgt cggctacgac
     6841 accgatcgca cctacgacgg cctgtatgca ggcaaccgcg tgacgctggg cgagttggcg
     6901 caggacttcg atgcgagcac cgatctgcat catctgccga acgtcggcaa cgatccgaac
     6961 ttcgcgttca tcctgcgcaa ccgcgactgg aagggtgatg aataatggaa ctccagtggc
     7021 ctttgatttt gttcaccacg ctggttgcgt ggagcgcggg gctgttcggg acgcaggcgc
     7081 tgatggcggt gttcggcgtc gggaagaagg cgcaggtgcc ggcgtgggtt gcgtcggccg
     7141 tcttgctggc ggccggcggg atcgcggtgt tcttccacct tgagcactgg gagcggatct
     7201 tcaacgggtt cgggcatttg acgagcggga tcacgcagga gctgatcgcg atcgtggtgc
     7261 ttgccgtggc cgccgtggcc tacctcgtgc tcatgcgcaa gtccgacgac ggcgcgagcg
     7321 tgccgaagtg gctggcgtgg ctgtccgtcg ccttgtcggt cgtgctggtc gccgtcatgg
     7381 cgcacagcta cacgatggcc gcccgccccg cctgggactc ggccctctgg atcctctacg
     7441 tcctcggcaa cgcctgcgtg ttgggccccg cgacgttcgc cctcctctcc accttggctg
     7501 cagggggacc ccgggaccaa cccgccgatc gcgcagccga cgccggggcc cccgcaggta
     7561 ggacggccct cgccggcgcc gccatcaacg ccctcgcaac cctcgccttc gccgccttcc
     7621 tccagctctc cgccggctcc ttcgccgacg tcggcctcta cttcgacccg acgcacccca
     7681 ccaaggccat ggccgacgca gccgcgacgg tcgcctccca ggccccgctg ctgtggctgg
     7741 gagccgtggc cgtgggcgcg atcgtgcccc tggccgccgc cttcatgggc aggcgcacgg
     7801 ggaactggag gctctgggcc ccggtcgcga tagccgccgc cctcgtcggc gccgtctgca
     7861 tgcgcgccgt cttctacaac ctggggctca gcgtgttcat gttctactag actagtcgac
     7921 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
     7981 aaagccagat tattaatccg gcttttttat tatttaattc ctgttaccgc tggttgccac
     8041 gacgccctcg aagtctcctt cgagggcgtt ctgtcgtttg caagaagggt ctggagaggc
     8101 aaatcgcgct cgaaggggct tcgaggccgg ggtaggtcgg gagggtggaa tccggatgtt
     8161 tcacgtgaaa catccgttct tttacggccg gtgtttcgcg tgaaacattt tgcggaaagg
     8221 actccttccc ttcccgtgcg ccaccccaac gccccgctcc tagcgctcgt acacctggtc
     8281 gcagttgagc agatccttca tggagtgcca gatctggtgc gggttgcccg gcgggttctt
     8341 gagcggcttc atcggatagc gctcctgcgc gcccgacagc agatcgacgg cgaacacttc
     8401 gtcggacagc tcgagcgcgc cttccgggca gatgtcggtg cagcagcggc acttcacgca
     8461 atcgaccgga cgatgcgtca cgccgatcga gccgtcctca cccgcgtact tcgcgatggc
     8521 cccggtgggg cagaacgtgg cgcacatctg gcacgacgag cacttctcgg gatcgatgat
     8581 cacatggccc cacaagcgcg tgtccatgag cacatcctgc ggctgcccca acgaatcgag
     8641 cgcggccagc aaacgttcgc ggcggtcggg aatgaagtga ggcagcgtgc cgtccttgcc
     8701 caccttcacg aacctcggct cccgccgctc ctccacaccc aatgcgtcct tgacggcgta
     8761 gtcagccgtc accgccgcaa cgttcttagc ctcgtccttc atgctggaga agaagctccg
     8821 acggctcgcg tcgtagccct cgtcgcccgc ccggcgcacg gatgagggaa gcttttccgc
     8881 caggtcgatg cgcatatcgt tgttccaggt tgcaagcagc gcgcttgccg tgtcgcacac
     8941 aacccgcgcc atctcgaaac ccgagacatg ctcgcattgc gcgcagcgcg cctgcacgag
     9001 gctcacgcgg cgcacgccca acgccgccag cgtgaccagc aggctctcct ccacgcgccc
     9061 caggcacgtg acgcccacca ccttctcggg gtcgtacaac ccgtcggccg cggcaagcag
     9121 ctgatcgcag gcgataacca cctgtccgtc ggccacgtca gccgccgcgc ggcatgcttg
     9181 cagaagctcg gcatcgttcg gacgatgcgc ttcaagcgcg caggtgggac acacggtggc
     9241 gcaggttccg cacccgatgc accgctcggg ttcgatgacc agctcgttgt cgtcatagga
     9301 gatgcaaccg ctcgtgcaca cgtcggcgca cttcatgcac gttgcgttgc gattgcgcac
     9361 aatagcgcaa cgaccctgat gaaccgtaat gtcggcgctc tgaagttgtt cgagcagacc
     9421 gaggaaattc gtcacgtgag ccatgacttc accctttctc gtctaccccc ttgcaccgca
     9481 cagtataacc actttcgccg tctcacgcga gaaaaaacgc gcttgacacc ttcgacaggc
     9541 ctccctatca tgtggccttg gggagtccgc cggcacgctg atcgcgtgcg aagcggacat
     9601 tgtttgcaaa agtgggaacc tcaggggtga ggtttcgcgg tgctgtgcgc cgtaagtgca
     9661 aacaatgtct gacgaggggc ggaacatgac tcacgattcc acgaaccagc atctttctct
     9721 tgagcccgac tacgacgggt tcgatccact tgaagatttc gaagacgatg cgaccgaggt
     9781 cgaaggccgt gccgacgagt tcgtcgccac ggtgagcgcc gccgaagacg cgctcgctca
     9841 cgacgcgatg gcacccgcca cgcccgccat ctgcgaaaag cccgctgaag agcgcatcgc
     9901 cgacttgttg gaggccatgg cgccgcgccg cacggtgctg ctgggcatcc tggccgcctg
     9961 cgccgagccg cagccggcat ccgccgtgaa cgctcgcgtc gacgagctgc aggagaacaa
    10021 cttctcggtg tacagcgcgg ccaacctgtg ctccttgctg gagaaggcgg gcgccatcga
    10081 gcgcgtcacg gccgagggcg aacccgcgga ggacatcgaa atcgagccac agaccgtcgt
    10141 cgtcgacggc gtcgaatacc tggaagcccg cgaacccgtc gagatctact ggcacatcac
    10201 cgagccgggc cgcgcggcgc ttgaggccga caaaccgctc gagcgcctgc gcgccctcct
    10261 ggacgaagat gccgcctacg cgcccatcta ccagcgcatc ctccgcttgt gcacagcaga
    10321 cggcggcgcc acgacacccg ccatcaacaa cgcggtcgac cacaacccgc tggtgcagaa
    10381 gccgcgcttc tacgcgcccc acttcgtcga ccgcctggag aaatgcgacg cgctggcctg
    10441 gaagaaggcg tggtgcatca ccgacatcgg acgcgccggc ctcgatatgc tggcagacgt
    10501 cgtagacgag aacgcgcccg ccacccagcc cgaaaccccc gcaacccctg atcccgccgc
    10561 atccaaggag gactaaccat gaccgaagca accgcgcccg caaccgagac cctcgccgac
    10621 gaagcgacca tgcaggagct cgtcaaactg aacgagcagc gcgccgccac gtacggcctg
    10681 ctgtcgcgcc tgtaccgcgt ggaggtggac cagccccttc tcgacgagct gcgcagcatg
    10741 cgcttccccg ccaagacggg caacgagaac gtggacgagg gctaccgcct gctggccacg
    10801 tacctgagca acacctggga caacagcgtc accgacctcg ccatcgacta cgtgcgcgtg
    10861 ttcatcggac acggcatcga cgcgttctcg gccgcctacc cgttcgaatc ggtgtacacc
    10921 tccgagaagc gcctgctcat gcaggaagca cgcgacgagg tgctggccat ctaccgcagc
    10981 gcaggcctcg acaagcagga cacatggaaa gagggcgagg atcatatcgc gctcgagctg
    11041 gaattcgagc agatcatggc cacgcgcacg gtcgaagcgc tgcgcaaagg cgacgaagac
    11101 gaggccgtcg cgctgctgac cacgcagaag aactttctcg aagaccatct ggcggcatgg
    11161 gctcccatga tgacctccga catgaagcga ttcgcgcaaa ccgacttcta ccggggcttg
    11221 gcgtacttga cggacgggtt cctccaaacc gaccaaacgt tcctcgacga cgtgctggcc
    11281 gaagactaac tgcacaggat cgaaagaagg agcgatggac aacaagcagg agaaaaccta
    11341 cgccggcatg acgcgacgca cgctgtgcct gggcgcaggc ggcgctgcgg tcatgctagg
    11401 tctaggcgga ttgaagtacg cgggatcgca accgctcgtg cgcccgccgg gaggccagga
    11461 cgaggaacgg ctcatctcgg cctgcatccg ctgcgagaag tgctacgaga tctgcccgcg
    11521 cgacgtcatc gcgcccgccc acctcgagga cggtgtgctg aacatgcgca cccccacgtt
    11581 cgacttctcg gccaactggt gcgactggtg cgccgaagag cacgacggca ccccgttgtg
    11641 cgtgagcgcc tgccccaccg aagcgctcaa gctgcccgac gacgccacgg ccgagaacac
    11701 catcatcggc aaagccgtta tcaacacgga ctggtgcttg gcgtacaagc tgatcggatg
    11761 ccggttttgc tacgacgcct gtccctacga ggccatggag ctcgacgaga acaaccgccc
    11821 cgtcgtgatc gaggacaagt gcaacggctg cggcgcgtgc gagagcgtgt gcgtctcgct
    11881 gcagaacggc tccatctccg aaggagccac cgcgcgcgcc atcgtcgtcc acccgcttga
    11941 cgaagttgaa ggataaggtg ccctcatgaa gaagaactcc aagaagctgc gcaccctcgt
    12001 cgcactggcc atcgtggccg tcgtcgccgt cggcttcgtc acgaacgtcg ggatcggcac
    12061 catgtcggca ccgggcatct gggacatctc catcctatgc ccgctgggcg ccttgggaac
    12121 gatgctcgcc tccaagatga tggtgccgcg cgccgtaatc tcgctgatcg tgatggtgct
    12181 gctcatcgtt ctgttcgccc gcgcgttctg cggatggatt tgccccgtgc cgctcgtgca
    12241 gaagttgcgc ggcttgttcg cgaagaagca acccgtcgaa cgcggcgacg atgccgatcc
    12301 tgccgcggcg ccgctcacga aagtcgagat cgacgcgctg ggcgtttcat gctcgaaggg
    12361 gtcgaagaat ggcagcggat gcgcgtcctg cgcctccaag cgcggagccg cgctcgatgc
    12421 tcgccacttc gtgctgggcg gcgcgctggc gtccaccttc atctttggat tcccggtgtt
    12481 ctgcctcgtc tgccccatcg gactcacgtt cgccaccatc ctgctggtca tcaacctgtt
    12541 ctcacacggc gatgtcacct ggtcgctcat cgtggtgccc gcgctgctta tcgccgaggt
    12601 gctgctgttc aagaagtggt gccataagct gtgcccgctt tcggcgttca tgagcctgat
    12661 cgcgaaggcg aacaagacct tcgtgcccac gatcgacgat gcgaagtgcc tggaaacggc
    12721 gaagggcggc gcgtgcgggg cctgcggccg cgtctgcgag gagggcatcg acccgcgcca
    12781 cccgcagctt tcgcaggcgg catggagcga gtgcaccaag tgccgcgcct gcgtggacgc
    12841 atgccctgcg aacgcgatca ccatgccgct gctgccgcgg cgaacgccgt ctaccgcaga
    12901 acctgtcgcc gtgccaacga acgccgagaa gatcgacgcg gagaatgcgc gccccgttcc
    12961 gtaaagcccc tcctgcgcga cccggctcgc agctgcagcg gtctggtcgc gcatccggaa
    13021 tcgggccgag cccgaccccg ggcaaaaggc ggcttggcaa gggctttggg ggacgagccg
    13081 ccttttgccc gggagcgaca tccccgctcc ccctccctgc gaaaagaggg atgccgcttc
    13141 cggccttcat ctgacaacca cgacgtggga ggacgaaaac ccatggcata cgaaacgaca
    13201 agcgaatcga ccgaggccct gctcgtcacc ggaggcgtcg gcaccggcaa aacgcaacgt
    13261 ctcgtcgagc gcgctgccgc gctgctggcc gaaaacgccg ccgcgggcaa cgtgctcgtg
    13321 ctgtgcgcga cgccccaggc cgcacgcctg ttctcggagc ggttgacagc ccgcgtcggt
    13381 gccaagcgcg ctgccggcgc aaccgtcgcc accgcacgcg ccttagcgct cgacgtgctg
    13441 gccgacgacg gcgcgcgccg ctggtcgggg cgcgagccgc gactactgac cgccttcgaa
    13501 gagacgttcc tcatggagga catgaaggtg agcgggctgc ggccgaagcg gctgcgcgag
    13561 atgctcaagt tcttctaccg ctcgtggacc gagctggcgg acgacgatcc cgattggctg
    13621 ctaccgggcg aggaggccga tgtccatacg ctgttgaaag agaacctggt cttcatgcgc
    13681 gccgtcatag aacccgaagc ggcgaacctt gctgcacgct acctccgaga acacgacgaa
    13741 gcgcgggcgg cgcattccta cgcgcacgtg ctgatggacg actacgcctg cgcaagccgt
    13801 gcctcgcaga cgctggcgaa tctcgtcgca acctgctcga tcaccgtggc gggcgacgcg
    13861 gaaacctgca tcgagacata cgattcgtac ccctatgccg ccggaatcga cgaattcctc
    13921 gcaacgcatc ccgatgctgc ttgcgagcac ctggatgccg acgcgcccga tgccgtcgaa
    13981 cgggtcgctc tggcaagcga cacgcccgaa gccgagttcg aacaggtggc gcgcacggtg
    14041 gccgaagccg tcgccgcagg aacgcccgca cggcagatcg tcgtggccgt accgaacagc
    14101 gtgtggtcgc gcaacatcgc aaccgctttg cacgcacgag gcgtgccaac cgaagcgctc
    14161 gccatctcgc agcccctgcg cggcgacgtg cgggacaacg cccgctgcac gcctgcgcgc
    14221 atcgccacgg ccctcaacct cgtggcgaac ccagacgaca ccgcggcttg gcggtgctgg
    14281 tgtggattcg gcgactacct ggtgaacagc gcggctttcg ccagcctgcg gacttgcgcg
    14341 aaggagcgca gtatcggcct cgtggacgct ttggagatgc taagcgaaaa cgactgcgag
    14401 cacgtagtgg gcgcgcaacg cgtggcgacc gcctacaagg acggtcgcgc gctcatcgag
    14461 caggtacgag gattgcgggg cacggcactg ctggacgagc tgacgcgcat cgtcaccgac
    14521 ggagcggaat cccaagcgcc cgccgttctg cgcgagctct gcctcgagca cggcaacgca
    14581 gccgatgaaa acccggaaag cctttacgat gccgatgctg cggcgatggc gcggcgcatg
    14641 gccgagcgcc tgctcgcgcc cacgctggag gttgccgacg cggtagccgt cgtcccctac
    14701 gacttcgcct gcggcttctc gcccgacctg ctgatcgtcg ccggcttcgt gaacggtttc
    14761 atcccctccc gcgactattt cgacgccacg gtgaccccgc ccgagcgcca ggagaagatg
    14821 cgcgccgtcg acgaacgacg cgtgcgcgcg ctcacaggaa aggccggccg cgcggtggcg
    14881 ctgtcgtact tcacgtccac cgacctcgaa agcgccggca agctgaagct caagatcagt
    14941 cgcatccgcc tcaagaacgg tatccgcacc tgcactatag agcccagcct attcctggag
    15001 caaatcgcag cgcaggagta gtaaaaaaag gatcaatttt gaactctctc ccaaagttga
    15061 tcccttaacg atccctgcag gagaaggcca tcctgacgga tggccttttt gaagctttct
    15121 agaatcgggg gcccccgggg ggattctaga acaatggaag tcctcgtctc gccctcgaat
    15181 tagcccgctt aatgagcggg ctttttttga attaattctc gcgagctggc acgacaggtt
    15241 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc gcgaattgca
    15301 agctggccga cgcgctgggc tacgtcttgc tggcgttcgg gagcagaaga gcatacatct
    15361 ggaagcaaag ccaggaaagc ggcctatgga gctgtgcggc agcgctcagt aggcaatttt
    15421 tcaaaatatt gttaagcctt ttctgagcat ggtatttttc atggtattac caattagcag
    15481 gaaaataagc cattgaatat aaaagataaa aatgtcttgt ttacaataga gtgggggggg
    15541 tcagcctgcc gccttgggcc gggtgatgtc gtacttgccc gccgcgaact cggttaccgt
    15601 ccagcccagc gcgaccagct ccggcaacgc ctcgcgcacc cgctggcggc gcttgcgcat
    15661 ggtcgaacca ctggcctctg acggccagac atagccgcac aaggtatcta tggaagcctt
    15721 gccggttttg ccggggtcga tccagccaca cagccgctgg tgcagcaggc gggcggtttc
    15781 gctgtccagc gcccgcacct cgtccatgct gatgcgcaca tgctggccgc cacccatgac
    15841 ggcctgcgcg atcaaggggt tcagggccac gtacaggcgc ccgtccgcct cgtcgctggc
    15901 gtactccgac agcagccgaa acccctgccg cttgcggcca ttctgggcga tgatggatac
    15961 cttccaaagg cgctcgatgc agtcctgtat gtgcttgagc gccccaccac tatcgacctc
    16021 tgccccgatt tcctttgcca gcgcccgata gctacctttg accaccatgg catcagcggt
    16081 gacggcctcc cacttgggtt ccaggaacag ccggagctgc cgtccgcctt cggtcttggg
    16141 ttccgggcca agcactaggc cattaggccc agccatggcc accagccctt gcaggatgcg
    16201 cagatcatca gcgcccagcg gctccgggcc gctgaactcg atccgcttgc cgtcgccgta
    16261 gtcatacgtc acgtccagct tgctgcgctt gcgctcgccc cgcttgaggg cacggaacag
    16321 gccgggggcc agacagtgcg ccgggtcgtg tcggacgtgg ctgaggctgt gcttgttctt
    16381 aggcttcacc acggggcacc cccttgctct tgcgctgcct ctccagcacg gcgggcttga
    16441 gcaccccgcc gtcatgccgc ctgaaccacc gatcagcgaa cggtgcgcca tagttggcct
    16501 tgctcacacc gaagcggacg aagaaccggc gctggtcgtc gtccacaccc cattcctcgg
    16561 cctcggcgct ggtcatgctc gacaggtagg actgccagcg gatgttatcg accagtaccg
    16621 agctgccccg gctggcctgc tgctggtcgc ctgcgcccat catggccgcg cccttgctgg
    16681 catggtgcag gaacacgata gagcacccgg tatcggcggc gatggcctcc atgcgaccga
    16741 tgacctgggc catggggccg ctggcgtttt cttcctcgat gtggaaccgg cgcagcgtgt
    16801 ccagcaccat caggcggcgg ccctcggcgg cgcgcttgag gccgtcgaac cactccgggg
    16861 ccatgatgtt gggcaggctg ccgatcagcg gctggatcag caggccgtca gccacggctt
    16921 gccgttcctc ggcgctgagg tgcgccccaa gggcgtgcag gcggtgatga atggcggtgg
    16981 gcgggtcttc ggcgggcagg tagatcaccg ggccggtggg cagttcgccc acctccagca
    17041 gatccggccc gcctgcaatc tgtgcggcca gttgcagggc cagcatggat ttaccggcac
    17101 caccgggcga caccagcgcc ccgaccgtac cggccaccat gttgggcaaa acgtagtcca
    17161 gcggtggcgg cgctgctgcg aacgcctcca gaatattgat aggcttatgg gtagccattg
    17221 attgcctcct ttgcaggcag ttggtggtta ggcgctggcg gggtcactac ccccgccctg
    17281 cgccgctctg agttcttcca ggcactcgcg cagcgcctcg tattcgtcgt cggtcagcca
    17341 gaacttgcgc tgacgcatcc ctttggcctt catgcgctcg gcatatcgcg cttggcgtac
    17401 agcgtcaggg ctggccagca ggtcgccggt ctgcttgtcc ttttggtctt tcatatcagt
    17461 caccgagaaa cttgccgggg ccgaaaggct tgtcttcgcg gaacaaggac aaggtgcagc
    17521 cgtcaaggtt aaggctggcc atatcagcga ctgaaaagcg gccagcctcg gccttgtttg
    17581 acgtataacc aaagccaccg ggcaaccaat agcccttgtc acttttgatc aggtagaccg
    17641 accctgaagc gcttttttcg tattccataa aacccccttc tgtgcgtgag tactcatagt
    17701 ataacaggcg tgagtaccaa cgcaagcact acatgctgaa atctggcccg cccctgtcca
    17761 tgcctcgctg gcggggtgcc ggtgcccgtg ccagctcggc ccgcgcaagc tggacgctgg
    17821 gcagacccat gaccttgctg acggtgcgct cgatgtaatc cgcttcgtgg ccgggcttgc
    17881 gctctgccag cgctgggctg gcctcggcca tggccttgcc gatttcctcg gcactgcggc
    17941 cccggctggc cagcttctgc gcggcgataa agtcgcactt gctgaggtca tcaccgaagc
    18001 gcttgaccag cccggccatc tcgctgcggt actcgtccag cgccgtgcgc cggtggcggc
    18061 taagctgccg ctcggacagt tcgaggctgg ccagcctgcg ggccttctcc tgctgccgct
    18121 gggcctgctc gatctgctgg ccagcctgct gcaccagcgc cgggccagcg gtggcggtct
    18181 tgcccttgga ttcacgcagc agcacccacg gctgataacc ggcgcgggtg gtgtgcttgt
    18241 ccttgcggtt ggtgaagccc gccaagcggc catagtggcg gctgtcggcg ctggccgggt
    18301 cggcgtcgta ctcgctggcc agcgtccggg caatctgccc ccgaagttca ccgcctgcgg
    18361 cgtcggccac cttgacccat gcctgatagt tcttcgggct ggtttccact accagggcag
    18421 gctcccggcc ctcggctttc atgtcatcca ggtcaaactc gctgaggtcg tccaccagca
    18481 ccagaccatg ccgctcctgc tcggcgggcc tgatatacac gtcattgccc tgggcattca
    18541 tccgcttgag ccatggcgtg ttctggagca cttcggcggc tgaccattcc cggttcatca
    18601 tctggccggt ggtggcgtcc ctgacgccga tatcgaagcg ctcacagccc atggccttga
    18661 gctgtcggcc tatggcctgc aaagtcctgt cgttcttcat cgggccacca agcgcagcca
    18721 gatcgagccg tcctcggttg tcagtggcgt caggtcgagc aagagcaacg atgcgatcag
    18781 cagcaccacc gtaggcatca tggaagccag catcacggtt agccatagct tccagtgcca
    18841 cccccgcgac gcgctccggg cgctctgcgc ggcgctgctc acctcggcgg ctacctcccg
    18901 caactctttg gccagctcca cccatgccgc ccctgtctgg cgctgggctt tcagccactc
    18961 cgccgcctgc gcctcgctgg cctgctgggt ctggctcatg acctgccggg cttcgtcggc
    19021 cagtgtcgcc atgctctggg ccagcggttc gatctgctcc gctaactcgt tgatgcctct
    19081 ggatttcttc actctgtcga ttgcgttcat ggtctattgc ctcccggtat tcctgtaagt
    19141 cgatgatctg ggcgttggcg gtgtcgatgt tcagggccac gtctgcccgg tcggtgcgga
    19201 tgccccggcc ttccatctcc accacgttcg gccccaggtg aacaccgggc aggcgctcga
    19261 tgccctgcgc ctcaagtgtt ctgtggtcaa tgcgggcgtc gtggcctgcc cgctctaatg
    19321 cccggttggc atggtcggcc catgcctcgc gggtctgctc aagccatgcc ttgggcttga
    19381 gcgcttcggt cttctgtgcc ccgcccttct ccggggtctt gccgttgtac cgcttgaacc
    19441 actgagcggc gggccgctcg atgccgtcat tgatccgctc ggagatcatc aggtggcagt
    19501 gcgggttctc gccgccaccg gcatggatgg ccagcgtata cggcaggcgc tcggcaccgg
    19561 tcaggtgctg ggcgaactcg gacgccagcg ccttctgctg gtcgagggtc agctcgaccg
    19621 gcagggcaaa ttcgacctcc ttgaacagcc gcccattggc gcgttcatac aggtcggcag
    19681 catcccagta gtcggcgggc cgctcgacga actccggcat gtgcccggat tcggcgtgca
    19741 agacttcatc catgtcgcgg gcatacttgc cttcgcgctg gatgaagtcg gccttggccc
    19801 tggccgattg gccgcccgac ctgctgccgg ttttcgccgt aaggtgataa atcgccatgc
    19861 tgcctcgctg ttgcttttgc ttttcggctc catgcaatgg ccctcggaga gcgcaccgcc
    19921 cgaagggtgg ccgttaggcc agtttctcga agagaaaccg gtaagtgcgc cctcccctac
    19981 aaagtagggt cgggattgcc gccgctgtgc ctccatgata gcctacgaga cagcacatta
    20041 acaatggggt gtcaagatgg ttaaggggag caacaaggcg gcggatcggc tggccaagct
    20101 cgaagaacaa cgagcgcgaa tcaatgccga aattcagcgg gtgcgggcaa gggaacagca
    20161 gcaagagcgc aagaacgaaa caaggcgcaa ggtgctggtg ggggccatga ttttggccaa
    20221 ggtgaacagc agcgagtggc cggaggatcg gctcatggcg gcaatggatg cgtaccttga
    20281 acgcgaccac gaccgcgcct tgttcggtct gccgccacgc cagaaggatg agccgggctg
    20341 aatgatcgac cgagacaggc cctgcggggc tgcacacgcg cccccaccct tcgggtaggg
    20401 ggaaaggccg ctaaagcggc taaaagcgct ccagcgtatt tctgcggggt ttggtgtggg
    20461 gtttagcggg ctttgcccgc ctttccccct gccgcgcagc ggtggggcgg tgtgtagcct
    20521 agcgcagcga atagaccagc tatccggcct ctggccgggc atattgggca agggcagcag
    20581 cgccccacaa gggcgctgat aaccgcgcct agtggattat tcttagataa tcatggatgg
    20641 atttttccaa caccccgcca gcccccgccc ctgctgggtt tgcaggtttg ggggcgtgac
    20701 agttattgca ggggttcgtg acagttattg caggggggcg tgacagttat tgcaggggtt
    20761 cgtgacagtt agtacgggag tgacgggcac tggctggcaa tgtctagcaa cggcaggcat
    20821 ttcggctgag ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa cacagtattg
    20881 caaggacgcg gaacatgcct catgtggcgg ccctcgcgag aattaattca gataaaaaaa
    20941 atccttagct ttcgctaagg atgatttcta gcgatgaccc tgctgattgg ttcgctgacc
    21001 gtagactggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg
    21061 tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga
    21121 cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac
    21181 gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg
    21241 cgacatcgcg cgcgcgcgcg cggatgctag cggcgcgcca aaaaaaagcc cgctcatta
//
