LOCUS       sequence-494337-        9457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFR-mEGFP in mammalian cells regulated by a synthetic 
            microRNA-based dosage compensation circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9457)
  TITLE     miRNA circuit modules for precise control of gene expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-synmiR-L-mRuby-Pro-EGFR-mEGFP-4×18
FEATURES             Location/Qualifiers
     source          1..9457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   651..780
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    795..843
                     /note="synthetic polyadenylation signal"
     ncRNA           complement(1047..1061)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(1223..1933)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     regulatory      1930..1939
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1955..2166)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        2211..2514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             join(2758..2829,2830..4692,4693..4761,4762..6387)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             join(6394..6396,6397..6399,6400..7113)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6439..6460)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6700..6719)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7047..7068
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7223..7344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7260..7279)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7314..7333
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7370..7510
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7370..7499
                     /label=AAV2 ITR
     rep_origin      7585..8040
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7672..7691)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7882..7903
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8057..8076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8176..8198
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8236..8254)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8322..8426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8427..8495,8496..9287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSLIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtcacaca aaaaaccaac acacagatct aatgaaaata aagatctttt
      841 attatttctt ccacaagcct gcaggaaaaa gaaagaggat gaatggtcag gaaaagtgat
      901 ttaatttata ccattttaat tcagctttgt aaaaatgtat caaagagata gcaaggtatt
      961 cagttttagt aaacaagata attgctcgaa ttctagcccc ttgaagtccg aggcagtagg
     1021 cataggaatt ataatgctta tctatacatc tgtggcttca ctatagataa gcattataat
     1081 tcctgcgctc actgtcaaca gcaatatacc ttctcgagcc ttctgttggg ttaagaagtc
     1141 gaagtaatcc cagcaagtgt ttccaagatg tgcaggcaac gattctgtaa agtactgaag
     1201 cctcattcaa acttacctgc tacttatata gctcgtccat gccaccacca aggttgctgt
     1261 atttggcaac tgccacttct ctttgcacta cgtaggtttc attgtcactc tcctcgatcc
     1321 tttccaggcg gtgatcaacg gcatggacac cgggcatctt gatgttcccg acggtctttt
     1381 ttgacctgta agttgtcacg aagttgcagt gcagatggcc accaccatca actttcagtg
     1441 cgatgtcagt gtatcctctc agaccaccat ctgctggata catcatctct gtattaggct
     1501 cccaaccctt ggtcttcttc tgcatcacgg gaccattgga gggaaagttt acccctctga
     1561 ccttgacgtt gtagacgagc tcgccatcct caaggctggt gtcctgcgtg acggtgacga
     1621 ctccaccatc ttcgtatctc gtaactcttt cccaagtaaa accctcagga aaggactgtt
     1681 taaagaaatc agggatgtcg gccgggtact tgataaaggt acggctgcca tacatgaacg
     1741 acgtggcaag aatgtcaaag gcaaatggca ggggtcctcc ctcgatgact ttgatcctca
     1801 tggtttgcac tccctcgtac ggtctgcctt ctccttcacc tgtgcatttg aattggtggc
     1861 cgttgaccga accttccatg accaccttca tacgcatatt ttccttgatc agctcttcgc
     1921 ccttagacac catggtggcg tcgactcacg acacctgtgt tctggcggca aacccgttgc
     1981 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
     2041 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
     2101 caccggttca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
     2161 ctgcccactg acgggcaccg gagccctatg acttgcatag ctgcgtatgg cgttacataa
     2221 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     2281 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     2341 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     2401 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     2461 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catgaattga
     2521 gggcgtcacc gctaaggctc cgccccagcc tgggctccac aaccaatgaa gggtaatctc
     2581 gacaaagagc aaggggtggg gcgcgggcgc gcaggtgcag cagcacacag gctggtcggg
     2641 agggcggggc gcgacgtctg ccgtgcgggg tcccggcatc ggttgcgcgc gcgctccctc
     2701 ctctcggaga gagggctgtg gtaaaacccg tccggaaacc acttaagctt cgccaccatg
     2761 agacctagcg ggacggcagg agctgccctg ttggccctat tggcggctct ttgcccagcc
     2821 agtcgggctc tggaggaaaa gaaagtttgc caaggcacga gtaacaagct cacgcagttg
     2881 ggcacttttg aagatcattt tctcagcctc cagaggatgt tcaataactg tgaggtggtc
     2941 cttgggaatt tggaaattac ctatgtgcag aggaattatg atctttcctt cttaaagacc
     3001 atccaggagg tggctggtta tgtcctcatt gccctcaaca cagtggagcg aattcctttg
     3061 gaaaacctgc agatcatcag aggaaatatg tactacgaaa attcctatgc cttagcagtc
     3121 ttatctaact atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag aaatttacag
     3181 gaaatcctgc atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa cgtggagagc
     3241 atccagtggc gggacatagt cagcagtgac tttctcagca acatgtcgat ggacttccag
     3301 aaccacctgg gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag ctgctggggt
     3361 gcaggagagg agaactgcca gaaactgacc aaaatcatct gtgcccagca gtgctccggg
     3421 cgctgccgtg gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc aggctgcaca
     3481 ggcccccggg agagcgactg cctggtctgc cgcaaattcc gagacgaagc cacgtgcaag
     3541 gacacctgcc ccccactcat gctctacaac cccaccacgt accagatgga tgtgaacccc
     3601 gagggcaaat acagctttgg tgccacctgc gtgaagaagt gtccccgtaa ttatgtggtg
     3661 acagatcacg gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat ggaggaagac
     3721 ggcgtccgca agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa cggaataggt
     3781 attggtgaat ttaaagactc actctccata aatgctacga atattaaaca cttcaaaaac
     3841 tgcacctcca tcagtggcga tctccacatc ctgccggtgg catttagggg tgactccttc
     3901 acacatactc ctcctctgga tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc
     3961 acagggtttt tgctgattca ggcttggcct gaaaacagga cggacctcca tgcctttgag
     4021 aacctagaaa tcatacgcgg caggaccaag caacatggtc agttttctct tgcagtcgtc
     4081 agcctgaaca taacatcctt gggattacgc tccctcaagg agataagtga tggagatgtg
     4141 ataatttcag gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa aaaactgttt
     4201 gggacctccg gtcagaaaac caaaattata agcaacagag gtgaaaacag ctgcaaggcc
     4261 acaggccagg tctgccatgc cttgtgctcc cccgagggct gctggggccc ggagcccagg
     4321 gactgcgtct cttgccggaa tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt
     4381 ctggagggtg agccaaggga gtttgtggag aactctgagt gcatacagtg ccacccagag
     4441 tgcctgcctc aggccatgaa catcacctgc acaggacggg gaccagacaa ctgtatccag
     4501 tgtgcccact acattgacgg cccccactgc gtcaagacct gcccggcagg agtcatggga
     4561 gaaaacaaca ccctggtctg gaagtacgca gacgccggcc atgtgtgcca cctgtgccat
     4621 ccaaactgca cctacggatg cactgggcca ggtcttgaag gctgtccaac gaatgggcct
     4681 aagatcccgt ccatcgccac tgggatggtg ggggccctcc tcttgctgct ggtggtggcc
     4741 ctggggatcg gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac gctgcggagg
     4801 ctgctgcagg agagggagct tgtggagcct cttacaccca gtggagaagc tcccaaccaa
     4861 gctctcttga ggatcttgaa ggaaactgaa ttcaaaaaga tcaaagtgct gggctccggt
     4921 gcgttcggca cggtgtataa gggactctgg atcccagaag gtgagaaagt taaaattccc
     4981 gtcgctatca aggaattaag agaagcaaca tctccgaaag ccaacaagga aatcctcgat
     5041 gaagcctacg tgatggccag cgtggacaac ccccacgtgt gccgcctgct gggcatctgc
     5101 ctcacctcca ccgtgcagct catcacgcag ctcatgccct tcggctgcct cctggactat
     5161 gtccgggaac acaaagacaa tattggctcc cagtacctgc tcaactggtg tgtgcagatc
     5221 gcaaagggca tgaactactt ggaggaccgt cgcttggtgc accgcgacct ggcagccagg
     5281 aacgtactgg tgaaaacacc gcagcatgtc aagatcacag attttgggct ggccaaactg
     5341 ctgggtgcgg aagagaaaga ataccatgca gaaggaggca aagtgcctat caagtggatg
     5401 gcattggaat caattttaca cagaatctat acccaccaga gtgatgtctg gagctacggg
     5461 gtgactgttt gggagttgat gacctttgga tccaagccat atgacggaat ccctgccagc
     5521 gagatctcct ccatcctgga gaaaggagaa cgcctccctc agccacccat atgtaccatc
     5581 gatgtctaca tgatcatggt caagtgctgg atgatagacg cagatagtcg cccaaagttc
     5641 cgtgagttga tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt
     5701 cagggggatg aaagaatgca tttgccaagt cctacagact ccaacttcta ccgtgccctg
     5761 atggatgaag aagacatgga cgacgtggtg gatgccgacg agtacctcat cccacagcag
     5821 ggcttcttca gcagcccctc cacgtcacgg actcccctcc tgagctctct gagtgcaacc
     5881 agcaacaatt ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg tcccatcaag
     5941 gaagacagct tcttgcagcg atacagctca gaccccacag gcgccttgac tgaggacagc
     6001 atagacgaca ccttcctccc agtgcctgaa tacataaacc agtccgttcc caaaaggccc
     6061 gctggctctg tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc gcccagcaga
     6121 gacccacact accaggaccc ccacagcact gcagtgggca accccgagta tctcaacact
     6181 gtccagccca cctgtgtcaa cagcacattc gacagccctg cccactgggc ccagaaaggc
     6241 agccaccaaa ttagcctgga caaccctgac taccagcagg acttctttcc caaggaagcc
     6301 aagccaaatg gcatctttaa gggctccaca gctgaaaatg cagaatacct aagggtcgcg
     6361 ccacaaagca gtgaatttat tggagcagga tctatggtga gcaagggcga ggagctgttc
     6421 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     6481 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     6541 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     6601 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     6661 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     6721 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     6781 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     6841 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     6901 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     6961 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     7021 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     7081 atcactctcg gcatggacga gctgtacaag tagagcttaa ttataatgct tatctaaata
     7141 aattataatg cttatctaaa taaattataa tgcttatcta aataaattat aatgcttatc
     7201 taaagggccc atcgatgcta gcaacttgtt tattgcagct tataatggtt acaaataaag
     7261 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7321 gtccaaactc atcaatgtat cttatcatgt ctgcggaccg agcggccgca ggaaccccta
     7381 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     7441 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     7501 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     7561 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7621 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
     7681 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7741 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7801 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7861 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     7921 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     7981 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     8041 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     8101 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     8161 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     8221 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     8281 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8341 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8401 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8461 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8521 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8581 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8641 ttctgctatg tggcgcggta ttatccctta ttgacgccgg gcaagagcaa ctcggtcgcc
     8701 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8761 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8821 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8881 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8941 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9001 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9061 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9121 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9181 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9241 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9301 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9361 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9421 gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
