LOCUS       sequence-494626-        9709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Arl13B (resistance to sgRNA) with deletion of Intrinsically 
            Disordered Region, tagged with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Mahajan D, Chia HM, Lu L
  TITLE     Knocking out ARL13B completely abolishes primary ciliogenesis in 
            cell lines.
  JOURNAL   Sci Rep. 2025 Jul 24;15(1):26980. doi: 10.1038/s41598-025-11124-5.
  PUBMED    40707593
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2025-07-24"; volume: "15(1):26980. doi"; pages: " 
            10.1038/s41598-025-11124-5"
COMMENT     Sequence Label: ARL13B-(Delta-IDR)-GFP
FEATURES             Location/Qualifiers
     source          1..9709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3810..3812,3813..3815,3816..4526)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3810..3812,3813..3815,3816..4526)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3855..3876)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4116..4135)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4463..4484
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4543..5042
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4578..4601)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4975..4994
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5063..5662
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5063..5082)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5559..5579
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5676..6264
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5729..5749)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6147..6158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6471..7104
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7233..7249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7233..7249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7246..7268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7257..7273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7281..7287,7288..7305,7306..7311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7326..7347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7464..7481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7635..8223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7715..7734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8394..9185,9186..9254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9017..9036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9255..9359)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9407..9541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9444..9463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9498..9517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9709,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat cgctagcgct accggactca gatctcgagg
     2821 ccaccatgtt cagtctcatg gcaagctgtt gtggatggtt taaacgctgg cgggagcctg
     2881 ttagaaaggt gactcttttg atggtgggac ttgataatgc tggtaaaacc gcaacagcaa
     2941 agggaatcca aggagaatac cctgaggacg tggcgccaac cgtcggattt tcaaaaatta
     3001 accttagaca gggaaagttt gaagtcacca tctttgactt gggaggtgga ataagaattc
     3061 ggggaatctg gaagaattac tatgctgaat cctatggggt aatatttgtt gtggattcca
     3121 gtgatgaaga gagaatggaa gagacaaaag aggctatgtc agaaatgcta agacatcctc
     3181 gtatcagtgg gaaacccata ttggtgttgg caaataaaca agataaagaa ggagcttcag
     3241 gagaagctga tgtcattgaa tgtctatctc tggaaaaatt ggtcaatgag cacaagtgcc
     3301 tgtgtcagat agaaccatgc tccgccattt ccggctatgg aaagaaaatt gacgagtcca
     3361 ttaaaaaagg cctttattgg ctgctacatg ttattgcaag agactttgat gccttaaatg
     3421 aacgcatcca aaaagagaca acagagcagc gtgctcttga ggaacaagag aaacaagaaa
     3481 gagctgaacg agtgcgaaaa ttacgagaag aaagaaaaca aaatgaacag gagcaggctg
     3541 aactcgatgg aaccagtggt agcggccgcc taagaatgaa aaggaaccac cgggtagaac
     3601 cacttaatat agatgactgt gctcctgaga gtccaacgcc acccccaccc cctcctcctg
     3661 ttggctgggg aacccctaaa gtcactagac ttccaaaact tgagcctctt ggtgaaacac
     3721 atcataatga tttctatagg aagccgctgc ctcccctggc tgtgccacag cgacctaaca
     3781 gtgatgctca tgatgtgatc tcaggatcca tggtgagcaa gggcgaggag ctgttcaccg
     3841 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     3901 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     3961 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     4021 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4081 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4141 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4201 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     4261 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     4321 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4381 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4441 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4501 ctctcggcat ggacgagctg tacaagtcta gataattcta ccgggtaggg gaggcgcttt
     4561 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     4621 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     4681 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     4741 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4801 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4861 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4921 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4981 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     5041 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5101 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5161 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5221 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     5281 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5341 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5401 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5461 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5521 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5581 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5641 gacccgcaag cccggtgcct gaccgcgtct ggaacaatca acctctggat tacaaaattt
     5701 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5761 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5821 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5881 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5941 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6001 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6061 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6121 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6181 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6241 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     6301 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     6361 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     6421 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     6481 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     6541 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     6601 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     6661 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     6721 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     6781 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     6841 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     6901 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6961 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7021 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7081 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7141 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg
     7201 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7321 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7381 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7441 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7501 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7561 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7621 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7681 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7741 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7801 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7861 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7921 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7981 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8041 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8101 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8161 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8221 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8281 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8341 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8401 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8461 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8521 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8581 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8641 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8701 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8761 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8821 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8881 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8941 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9001 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9061 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9121 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9181 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9241 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9301 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9361 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
     9421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     9541 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     9601 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     9661 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagtt
//
