LOCUS       sequence-494851-       11875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct for N-terminal S 
            protein-Flag-Streptavidin binding peptide (SFB)-tagged ZNF451.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Zhang H, Xiong Y, Sun Y, Park JM, Su D, Feng X, Keast S, Tang M, 
            Huang M, Wang C, Srivastava M, Yang C, Zhu D, Chen Z, Li S, Yin L, 
            Pommier Y, Chen J
  TITLE     RAD54L2-mediated DNA damage avoidance pathway specifically preserves
            genome integrity in response to topoisomerase 2 poisons.
  JOURNAL   Sci Adv. 2023 Dec 8;9(49):eadi6681. doi: 10.1126/sciadv.adi6681. 
            Epub 2023 Dec 6.
  PUBMED    38055811
REFERENCE   2  (bases 1 to 11875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-12-8"; volume: "9(49):eadi6681. doi"; pages: " 
            10.1126/sciadv.adi6681. Epub 2023 Dec 6"
COMMENT     Sequence Label: pLEX307-SFB-ZNF451
FEATURES             Location/Qualifiers
     source          1..11875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1282..1326
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1360..1383
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1387..1410
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1420..1533
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    1573..1597
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4784..4808)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4816..4857
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4908..5496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4961..4981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5379..5390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5568..5801
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5879..6000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5916..5935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5970..5989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6040..6175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6195..6214)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6196..6214)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6224..6241)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6224..6240)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6233..6255)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6382..6837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6469..6488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6679..6700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6863..6967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6968..7036,7037..7828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7186..7205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7999..8587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8488..8507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8741..8758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8875..8896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8911..8916,8917..8934,8935..8941)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8949..8965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8954..8976
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8973..8989
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8973..8989
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9008..9028
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9010..9028
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9056..9282
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9283..9463
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9510..9635
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10128..10361
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10546..10590
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10821..11031
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10843..11039
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10890..11025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11071..11673
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11071..11090)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11567..11587
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11716..11833
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctat gaaagaaacc gctgctgcta aattcgaacg ccagcacatg
     1321 gacagcggag ccggcgcagg agccggcgct gacgcgcctg actacaaaga cgatgacgac
     1381 aagggagatt acaaggatga cgatgacaaa ggcgcgtcga tggacgagaa gaccaccggc
     1441 tggcggggcg gccacgtggt ggagggcctg gccggcgagc tggagcagct gcgggccagg
     1501 ctggagcacc accctcaggg ccagcgggag cccgatagtg ctggtagtgc tggtagtgct
     1561 ggtgattcat caacaagttt gtacaaaaaa gcaggcttca tgggagaccc ggggtcggag
     1621 ataatagaat ctgtccctcc agctggccct gaggcatctg agtcaacaac ggatgaaaat
     1681 gaagacgaca ttcagtttgt cagtgaagga ccattacgac ctgttcttga atacattgat
     1741 ctggtcagca gtgatgatga agagcctagc acctcttata ctgatgagaa tattaaacgt
     1801 aaagaccata ttgattatca gaaggataaa gttgctttaa ctctggctcg tctagcccgc
     1861 catgttgaag tggagaaaca gcagaaagaa gagaagaata gagcattcag agaaaaaatt
     1921 gattttcagc atgctcatgg gttacaagaa ttggaattta ttcgaggaca ttctgataca
     1981 gaagcagcaa gactgtgtgt ggaccagtgg ctaaaaatgc caggactcaa aacaggcaca
     2041 attaattgtg gaacaaaaag ttcattccga agaggaggcc acacgtgggt gtctgggaaa
     2101 ccaattttat gtcctataat gcactgtaac aaggagtttg acaatgggca ccttctctta
     2161 ggacatttga aaaggttcga tcactctcca tgtgatccaa caattacact acatggacct
     2221 ttcttcagct cctttgcttg tgtagtatgt tataaaaaat ttgttactca acaacaatat
     2281 agagatcacc tttttgataa ggaagccaca gatgatggac ataacaacaa ccttcttcct
     2341 cagattattc agtgttttgc atgtccaaat tgcttccttc tttttagcag aaaggaggag
     2401 tgttcaaagc atatgtctgg aaagaatcat ttccatcaga gtttcaaact gggtgataac
     2461 aaaggaattg cacatccaat atctttccca tcttttgcaa agaaactttt gatctctctg
     2521 tgcaaagatg ttccctttca agttaagtgt gtggcctgcc acaagacact gcgttcccac
     2581 atggagctca ctgcccattt cagagttcat tgtcgaaatg ctggacctgt agctgtagct
     2641 gagaagagca ttacccaggt tgcagagaaa ttcatattaa gaggttattg tccagattgc
     2701 aatcaagtct ttgtggatga aaccagcacc caaaatcata agcagaattc aggacacaaa
     2761 gtccgagtca ttaactcagt ggaagaatca gtcttactct attgccacag cagcgaaggg
     2821 aacaaggatc cttcttctga cttgcattta ttgttggatc aatcaaaatt ttcatcactt
     2881 aaaagaacca tgtctattaa agaatctagc tcactggagt gcattgccat tccaaaaaag
     2941 aagatgaatt taaaagataa aagccatgaa ggtgttgctt gtgtccagaa agaaaaatca
     3001 gtagttaaaa cctggttctg tgaatgcaat cagcgattcc caagtgaaga tgcagtagaa
     3061 aagcatgttt tctcagcaaa cacaatgggt tataaatgtg tggtctgtgg aaaggtatgt
     3121 gatgattcag gggtcattcg tttacacatg agccggattc acggaggggc acatttaaat
     3181 aactttcttt tctggtgtcg gacatgcaaa aaggagttaa caaggaaaga tactatcatg
     3241 gcacatgtga ctgaatttca taatggacac agatattttt atgagatgga tgaggtagaa
     3301 ggtgaaactt tgccatcatc ctctacaaca ttggataatt tgactgctaa caagccttca
     3361 tcagctatta ctgttattga tcattccccg gcaaatagtt ctccgagggg taaatggcaa
     3421 tgccggattt gtgaagatat gtttgattcc caggaatatg taaaacagca ctgcatgtct
     3481 ttggcaagcc acaagtttca tagatacagc tgtgctcact gcagaaagcc ttttcataag
     3541 atagaaacat tgtaccgaca ttgccaagat gagcatgaca atgagataaa gattaaatac
     3601 ttctgtgggc tttgtgatct tatctttaat gtggaagaag catttctgag tcattatgag
     3661 gagcaccaca gcatagatta tgtatttgtg tcagaaaaaa ctgaaacttc aattaaaacc
     3721 gaagatgatt ttccagtaat agagaccagt aaccagttaa cttgtggttg ccgtgagagt
     3781 tacatctgta aagtcaacag aaaagaagat tatagcagat gtctccaaat catgctggat
     3841 aaaggaaaac tgtggtttcg ctgcagttta tgttcggcaa cagcacagaa tttaaccgac
     3901 atgaacactc atatccatca agtgcacaaa gaaaagagtg atgaggagga gcagcagtat
     3961 gtaatcaagt gtggcacctg caccaaagca tttcatgatc ctgagagtgc acagcagcat
     4021 ttccatagaa aacattgctt cttacagaaa cccagtgtgg ctcattttgg atctgaaaaa
     4081 tcaaacctgt acaagtttac tgctagtgcc tcacatacag agagaaaact gaaacaggca
     4141 ataaactatt caaaaagttt agacatggag aaaggagttg agaatgacct aagctatcag
     4201 aatatagagg aagaaattgt tgagcttcca gatttggatt acctgcgaac catgactcat
     4261 atagtctttg tagattttga taactggtca aacttttttg gtcatctacc agggcatcta
     4321 aaccaaggaa catttatttg gggctttcaa ggaggaaaca ccaattggaa gcctccgctc
     4381 aactgtaaga tttataacta cctgaacagg attggatgct tcttccttca tcctcgctgt
     4441 agtaaaagaa aagatgctgc tgattttgcc atatgtatgc atgctggccg tctagatgaa
     4501 caactaccca agcaaattcc tttcaccatc ctctcaggag atcaaggttt tctggagcta
     4561 gagaatcaat ttaagaagac tcagaggcca gctcatatac taaaccctca ccacttagag
     4621 ggagatatga tgtgtgcctt gttaaatagc atatctgata ccaccaaaga atgtgacagt
     4681 gatgataaca tgggtgccaa aaatacttca ataggagaag aatttatatc cacagaagat
     4741 gtggaattag aagaagctat tagaagaagt cttgaggaaa tgaacccagc tttcttgtac
     4801 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     4861 taatgaacta gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacctctgg
     4921 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4981 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5041 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5101 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5161 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5221 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5281 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5341 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5401 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5461 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     5521 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5581 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5641 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5701 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5761 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     5821 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6001 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     6061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     6181 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6241 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6301 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6361 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6721 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     6841 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6901 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6961 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7021 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7081 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7141 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7201 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7261 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7321 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7381 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7441 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7501 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7561 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7621 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7681 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7741 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7801 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7861 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7921 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7981 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8041 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8101 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8161 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8221 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8281 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8341 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8401 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8461 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8521 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8581 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8641 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8701 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8761 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     8821 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     8881 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     8941 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     9001 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     9061 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     9121 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     9181 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     9241 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     9301 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9361 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9421 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     9481 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     9541 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     9601 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     9661 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     9721 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     9781 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     9841 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     9901 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     9961 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
    10021 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    10081 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    10141 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    10201 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    10261 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    10321 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    10381 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    10441 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    10501 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    10561 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    10621 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    10681 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    10741 cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg
    10801 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag
    10861 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
    10921 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
    10981 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
    11041 agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc
    11101 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
    11161 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
    11221 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
    11281 gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
    11341 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
    11401 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
    11461 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
    11521 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
    11581 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
    11641 tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc
    11701 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
    11761 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
    11821 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccgg
//
