LOCUS       sequence-494915-       14648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14648)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 14648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-DLL4-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..14648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7570..7586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        7753..8132
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8133..8336
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8287..8307
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8333..8357
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        8567..8585
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8567..8584
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     8598..8617
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             10253..10288
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             10298..10321
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             11981..12025
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             12026..12052
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(12192..12209)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12198..12309
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12427..12447)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12432..12761
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12612..12747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12674..12693
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12809..12856
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12875..13474
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12875..12894)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13371..13391
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    13604..13725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13641..13660)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13695..13714
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(13778..14569,14570..14638))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14401..14420
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(14639..14648,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactag gggtttgagg gccaatggaa cgaaaacgta
     1561 cgtttatggt ataacttatt gattataata cagtatacaa aggtcccttt tcagggaccc
     1621 tttcgtatgt tgtagctgat tttattttct tcttttcttt tgggtgcttt taatagccat
     1681 atatgatgtt tcatataaag ttaaaagttt ccccatctgt ctatctatag cgtcatgttt
     1741 ttcaggattt cttaatttct gcagcagttt agatatatat ttcgcaggta actttattat
     1801 taatgaatta tgggattgat acatttcctt tgtccaatct aaatcttttc ctgatttcaa
     1861 tacctcactc tcatctctat taattataaa accgagcttt ccatacggac ctgttaaata
     1921 tgattgtaat tgtctatact cagatggacc taattcttca aaatttttag catcaaaaac
     1981 aacttgtctt gttttatagt cctccaatac tcgtttccaa aaatcagatt tgccaccatt
     2041 ggtgcctata atatctcgtc tctgaactgc gttaccattt ggatgggact tgatgtctgt
     2101 taggtgggat gcaaatacta ttcttagtgc gtctaaacac cattgttcaa attcagtggc
     2161 accttcattt cctatcggta tctgatctag atgagtagtt atttgaccta ttgttttatt
     2221 tctaatggct gaattatctg aaataatatt aatatcatat tcgtcattta tctcctctgc
     2281 ttcctctgga gcaagtgcat tacgatttag attcaaacca agccagtaac atggatgaat
     2341 taataatttc tcattacttt caaaaccttt atctggagtt cgcccgtcat ggcaaaatga
     2401 ataggatgag gtgtttttat cacgaatacc aacaaatcct acactataca aactttggag
     2461 aattccactt gcctttaaca actgtatttc agacgttatt ttaggatctc cattttcttc
     2521 gattaattcg aaagatgctt ctattttttt taagcacgta taaactgtta attcaggttc
     2581 aatgctacga aatgcactag ttataacctg tattgaagga aagatcttct gatactcttt
     2641 ccagagatct tcaagtctgg ccatggaaat tgacttggct gcatattcta ggtcagtgtt
     2701 tatgatagtt tctctattct ctctgaatgc ggaaaaaaaa gcttcattca acaatgatag
     2761 taaatccctg ggccggtaaa gggtaaattg caaacatcgc ttaaaaccat tcctcccttt
     2821 aagatcatcc gctgtgcatc tatcccaaac tcgttgatct ttctcaatat ctagcttaaa
     2881 tgctactttc attcttttag ctgacagcat taggagttgt gcccagtccc aatgcaacct
     2941 tatgacttga ccctctatat ttctcgagta atcaggatct tcctttgata gcgacctaaa
     3001 tatattatcc cttaaaaaaa ttattggacg aatgcatttt gctttttgat ttaattcaat
     3061 agatgcatat gctagacctg caatgattcc aattcctata ttatccggtt catacgcctc
     3121 atctagctta tccattaata tgacaacttt cctgtctgag cgttcaagaa gtgatactat
     3181 attattttct atttctgaga tattcaaatt gaattgaaga tcaccaattg attcttctgg
     3241 gttattttca tctaaatact cctttgcgac aagcctacac tttcttaaaa tgtcaccttg
     3301 tgcagaattc cattttttca aatgttcatt caacaatgtt tctgatgata tttgagatga
     3361 caatttgtaa tgagatgata tatatgatgc tatctccatt agcatagcgt atcgccataa
     3421 tagtcttgtt gctgcccttg ctaaattaaa tgatcctgta aatggtttca acattgatct
     3481 gaaaccaata atttgagaat cgtctggtga gaaactcagg attaatattt ttttgtcttt
     3541 cttccaatgc tcatttagct gaataaataa agcactttta cctgtccctc gtctaccaac
     3601 aacaatggtc ctgtcatcag tttcaattag agtcctaaag tcagcagttt caatgaaagc
     3661 attactcaac atctttttat cattttctgc tgtcgtatca ccaaacggat tagactttga
     3721 agtaatattc aattccatat tcaacctttt atgttagttg ctttcattta ttactttata
     3781 tactgttgaa cgagcaatat tcattgtttt tgatatatgt gaggcaccta acccctgttg
     3841 ccacatattt aatactgcat ctctatctat ttttcttttt ctaccaaaaa caactccttt
     3901 tgccattgcc tcttggcgac cttcattggt acgctctagt attctctgtc gttctgcctg
     3961 ggccactgca gatagaatag tgacaaccat tttacccatc tccccatcgg tactgattcc
     4021 gtcatcaata aaccgaatgg atacaccttg ggcgtcaaac tcttttatta actggatcat
     4081 gtcagcagta tcgcgcccaa ggcggtcaag tttcttcacc aagatgacgt caccttcctc
     4141 caccttcatc ctcagcaagt ccagcccttt ccgatcgctt gaactgcccg atgccttgtc
     4201 agtaaagatg cgatttgctt tcacgcctgc gtctttgagt gcccgaacct gaatatcgag
     4261 agattgctgg ctggttgata cccgtgcgta accaaaaagt cgcataaaaa tgtatcctaa
     4321 atcaaatatc ggacaagcag tgtctgttat aacaaaaaat cgatttaata gacacattaa
     4381 cagcactgtt tttatgtgtg cgataattta taatatttcg gacggttgca aaattgttaa
     4441 tgaataactg tcaggtaggg agcctgatat gcctgtcgac tttctaacct ctgaccaaaa
     4501 acagaattac ggttgttatg ctgcagaacc taatgacgtg caactggcgc gctattttca
     4561 tcttgatgaa cgggatctgg ccttcattaa ccaacgacgg ggcaaacata ataggctggg
     4621 cattgcgctt cagctcacca cagcccgttt tctgggaaca tttctgacgg atttaactca
     4681 ggttctgcct ggtgttcaac attttgtcgc ggtacagctt aatatccacc gtccagaagt
     4741 tctctcccgc tatgctgaac gggacactac ccttagagaa catactgcat taattaagga
     4801 atattacggc tatcatgaat ttggtgattt tccatggtct ttccgcctga agcgtctgct
     4861 atatacccgg gcgtggctca gtaatgagcg accgggtctg atgtttgatt ttgccactgc
     4921 atggttgctt caaaataagg tattactgcc cggagcaacc acactagtac gtctcatcag
     4981 tgaaattcgt gaaagggcaa atcagcggct gtggaaaaag ctggccgcac tgccgaacaa
     5041 atggcaggca gctcaagtga tggagcttct ggtcattccg gaaggtcagc gtgtatcagc
     5101 actggaacag ttgaaaaaag gccctgttac agtcagtgga cctgcgttta atgaagcgct
     5161 ggaacgatat atccgattac gaagtcttga gttttcccga ctgaactttt ccggtctgcc
     5221 tgccattcaa ctgcgtaatc tggctcgtta tgctggcatg gcgtcggtaa aatatatcgc
     5281 tcgaatgcca cagcagagaa agcttgctgt acttactgca ttcgttaaag cacaggaaat
     5341 aacggcatta gacgatgccg ttgatgtgct tgatatgcta attctggaca ttatccgcga
     5401 agcaaagaaa accgggcaaa aaaaaagact caggacactg aaagatcttg atcaggccgc
     5461 attgttactg gcgcgggcat gtgcattgtt gctggatgat aatacagatg tcccagatct
     5521 caggcaggtt atcttcaagt gcgtacccaa aaacagactg gcagaatctg taagcaaggt
     5581 taatgaactt gctcgtccac agaacaataa tttccatgac gaaatggttg agcagtacgg
     5641 tcgggttaaa cgttttcttc cggcggtgtt gcgggacctg catttccgtg cggcaccggc
     5701 aggtgaacat gtactggctg cgattcatta tctggcagaa ctgaatggtt cgaaaaagcg
     5761 catccttgat gatgcgcctg aacatattat caccggtccc tggaaacgcc tcgtatacga
     5821 tgcggaggga cggatacagc gtgcaggtta ttcactatgt ttgctggaac gccttcagga
     5881 tgcactgcgc cgccgggaca tctggcttga aaacagtgat cgctggggag atcctcgcga
     5941 gaagttgttg caaggtgaag agtggcagac tcagcgtatt cctgtctgtc gggcactggg
     6001 acatcctgtc gatggacgta aaggtgtgca acaactggct attcagctgg atgagacctg
     6061 gaaagccgtg gcatcacgat ttgaaaagaa tgcggaagtt catatctgta atgaaggtaa
     6121 atatccatcc ctgactatca gttgtctgga gaaacaggaa gagccaccat cattgcttcg
     6181 tctaaataat cggatcaaac agctactccc accggtagat ttaacggaac tgttacttga
     6241 gatagatgcc cagacaggat ttacacatga gtttgcgcat gtcagtgaat ctggtgctcg
     6301 agcgcaagat ttgcacatca gtttatgtgc ggtattgatg gctgaagcct gtaatatcgg
     6361 actggaaccg ctgataaagc acaatatacc agcactgacc cgccatcggc tcagttgggt
     6421 gaaacagaat taccttcgtg cagaaacgct ggtcagcgcc aatgcccgcc tggttgattt
     6481 tcagtccaca ctggagcttg ctggtcgttg gggaggtgga gaagtggcat cagctgacgg
     6541 catgcgcttt gtcacaccag tgaagaccat caactcagga tctaacagaa aatattttgg
     6601 ttctggacga ggcatcacct ggtataactt cgtatctgat cagtactctg ggttccatgg
     6661 cattgtggta cccggtacat tacgggattc gatttttgta ctggaaggac ttcttgagca
     6721 gcagacaggg ctgaatccag ttgaaatcat gacagacact gcgggtagca gcgatattat
     6781 tttcggtctg ttctggctac tggggtatca gttttccccc cggcttgccg atgcaggtga
     6841 ggcggtgttc tggcgggtga ataaatcggc aaactacgga gtactggata agttggcccg
     6901 aggatatgca gatctgtcaa aagcggagag tcagtgggat gagatgatgc gaaccgctgg
     6961 ttcgctgaag ttgggtacaa ttcatgcgtc agaactcatt cgctctttac tgaaaagctc
     7021 gcgcccatca ggactggcac aggcaattat ggaagtaggg cgtgtaaaca aaacgctata
     7081 tctcctcaat tatattgatg atgaagatta tcgtcggcgg atcctgacgc agcttaatcg
     7141 aggagaaggc cgccacgctg tggctcgtgc gatttgttac ggacaacgcg gagagatcag
     7201 aaagcgttat cgtgaagggc aggaagatca actgggggcg ctgggcctgg ttactaatgc
     7261 agtggttctg tggaacacgc tctatatgga ggaagcgttg agctggatgc gccgtaatgg
     7321 cgaagaaatt atagatgaag atatcgctcg gctatctccc ctgatgcacg ggcatatcaa
     7381 tatgttgggc cattatacat tcacgttgcc agaggatatt ttaaaagggg aactgagagc
     7441 tctaaattta aatataaaca acgaattatc tccttaacgt acgttttcgt tccattggcc
     7501 ctcaaacccc aactataaga tgttatcagt atttattatc atttagaata aattttgtgt
     7561 cgcccttaat tgtgagcgga taacaattac gagcttcatg cacagtggcg ttgactcaat
     7621 attggccatt agccatatta ttcattggtt atatagcata aatcaatatt ggctattggc
     7681 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     7741 gaccgccatg ttggcattga ttattgacta gttattaata gtaatcaatt acggggtcat
     7801 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     7861 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     7921 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     7981 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     8041 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     8101 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     8161 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     8221 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     8281 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     8341 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     8401 accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg attccccgtg
     8461 ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg gcttcgttag
     8521 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact
     8581 atagaataac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
     8641 cctcggttct atcgaaaacg cgcctgctag cgccaccatg aagaccatca ttgccctgag
     8701 ctacatcttc tgcctggtgt tcgctgcggc cgccagcgga gtttttcagc tgcaactgca
     8761 agagttcatc aacgagagag gcgtgctggc ctctggcaga ccttgtgaac ctggctgccg
     8821 gaccttcttc agagtgtgcc tgaagcactt ccaggccgtg gtgtctcctg gaccttgtac
     8881 ctttggcacc gtgtctacac ctgtgctggg caccaatagc ttcgccgtgc gggatgattc
     8941 tagcggcgga ggcagaaatc ctctgcagct gcccttcaac ttcacctggc ctggcacctt
     9001 cagcctgatc atcgaagcct ggcatgcccc tggggatgat ctgaggcctg aagctttgcc
     9061 tcctgacgct ctgatcagca agatcgccat ccagggatct ctggccgtgg gccaaaattg
     9121 gctgctggac gagcagacca gcacactgac cagactgcgg tacagctaca gagtgatctg
     9181 cagcgacaac tactacggcg acaactgcag cagactgtgc aagaagcgga acgaccactt
     9241 cggccactac gtgtgtcagc ccgatggcaa cctgtcttgt ctgcctggat ggaccggcga
     9301 gtactgccag caacctatct gtctgagcgg ctgccacgag cagaatggct actgttctaa
     9361 gcccgccgag tgcctgtgta gacctggctg gcaaggcagg ctgtgcaacg agtgcatccc
     9421 tcacaatggc tgcagacacg gcacctgtag caccccttgg cagtgcacat gtgacgaagg
     9481 atggggcgga ctgttctgcg accaagacct gaactactgc acccatcaca gcccctgcaa
     9541 gaacggcgcc acatgtagca acagcggcca gcggtcttac acctgtacct gcagacctgg
     9601 atacaccggc gtggactgcg agctggaact gagcgagtgc gacagcaacc cctgtagaaa
     9661 tggcggctcc tgcaaggacc aagaggacgg ctatcactgc ctgtgtccac ctggatatta
     9721 cggcctgcac tgcgaacaca gcaccctgag ctgtgccgac tctccctgtt ttaatggcgg
     9781 cagctgtaga gagagaaacc agggcgccaa ttacgcctgc gagtgcccac ctaatttcac
     9841 cggcagcaac tgcgagaaga aagtggaccg gtgcaccagc aatccctgcg ctaatggtgg
     9901 ccagtgcctg aacagaggcc ccagcagaat gtgtagatgc agacccggct ttaccggcac
     9961 ctattgcgaa ctgcacgtgt ccgactgcgc ccggaatcct tgtgctcatg gcggcacatg
    10021 ccacgacctg gaaaacggcc tgatgtgcac ttgccctgcc ggcttctctg ggagaagatg
    10081 tgaagtgcgg accagcatcg acgcctgtgc cagctctcct tgcttcaaca gagccacctg
    10141 ttacaccgac ctgagcaccg acaccttcgt gtgcaactgc ccttacggct tcgtgggcag
    10201 cagatgcgag tttcctgttg gcctgcctcc tagcttccct tggggcgcgc cggaagatca
    10261 agttgatcca agattgattg atggcaaggg cggatctctg gaggtgctgt tccagggacc
    10321 aggcggaagc ggcaatcccg gcggcgatct ggccccccag atgctgcgcg agctgcagga
    10381 gaccaatgcc gccctgcagg acgtgcgcga gctgctgcgc caccgcgtga aggagatcac
    10441 cttcctgaag aacaccgtga tggagtgcga cgcctgcggt atgcagccct ctagaggctc
    10501 cggctccatc atcccagttg aggaggagaa cccggacttc tggaaccgcg aggcagccga
    10561 ggccctgggt gccgccaaga agctgcagcc tgcacagaca gccgccaaga acctcatcat
    10621 cttcctgggc gatgggatgg gggtgtctac ggtgacagct gccaggatcc taaaagggca
    10681 gaagaaggac aaactggggc ctgagatacc cctggccatg gaccgcttcc catatgtggc
    10741 tctgtccaag acatacaatg tagacaaaca tgtgccagac agtggagcca cagccacggc
    10801 ctacctgtgc ggggtcaagg gcaacttcca gaccattggc ttgagtgcag ccgcccgctt
    10861 taaccagtgc aacacgacac gcggcaacga ggtcatctcc gtgatgaatc gggccaagaa
    10921 agcagggaag tcagtgggag tggtaaccac cacacgagtg cagcacgcct cgccagccgg
    10981 cacctacgcc cacacggtga accgcaactg gtactcggac gccgacgtgc ctgcctcggc
    11041 ccgccaggag gggtgccagg acatcgctac gcagctcatc tccaacatgg acattgacgt
    11101 gatcctaggt ggaggccgaa agtacatgtt tcgcatggga accccagacc ctgagtaccc
    11161 agatgactac agccaaggtg ggaccaggct ggacgggaag aatctggtgc aggaatggct
    11221 ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact gagctcatgc aggcttccct
    11281 ggacccgtct gtgacccatc tcatgggtct ctttgagcct ggagacatga aatacgagat
    11341 ccaccgagac tccacactgg acccctccct gatggagatg acagaggctg ccctgcgcct
    11401 gctgagcagg aacccccgcg gcttcttcct cttcgtggag ggtggtcgca tcgaccatgg
    11461 tcatcatgaa agcagggctt accgggcact gactgagacg atcatgttcg acgacgccat
    11521 tgagagggcg ggccagctca ccagcgagga ggacacgctg agcctcgtca ctgccgacca
    11581 ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg agctccatct tcgggctggc
    11641 ccctggcaag gcccgggaca ggaaggccta cacggtcctc ctatacggaa acggtccagg
    11701 ctatgtgctc aaggacggcg cccggccgga tgttaccgag agcgagagcg ggagccccga
    11761 gtatcggcag cagtcagcag tgcccctgga cgaagagacc cacgcaggcg aggacgtggc
    11821 ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc gtgcaggagc agaccttcat
    11881 agcgcacgtc atggccttcg ccgcctgcct ggagccctac accgcctgcg acctggcgcc
    11941 ccccgccggc accaccgacg ccgcgcaccc gggtggcgga ggactgaacg acatcttcga
    12001 ggctcagaaa atcgaatggc acgaacatca ccatcaccat caccatcacc attaaggccg
    12061 gcctgtagtc actggcacaa cgcgttaccc ggtaagccaa tcgggtatac acggtcgtca
    12121 tactgcagac agggttcttc tactttgcaa gatagtcttg agtagtaaaa taaatagata
    12181 gagaaaatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
    12241 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
    12301 aattgcatca caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    12361 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
    12421 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
    12481 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
    12541 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
    12601 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
    12661 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
    12721 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
    12781 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
    12841 catagtataa tacgacaagg tgaggaacta aaccatgacc gagtacaagc ccacggtgcg
    12901 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
    12961 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
    13021 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    13081 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    13141 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    13201 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    13261 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    13321 ggcggccgag cgcgccgggg tgcccgcctt cctggagaca tccgcgcccc gcaacctccc
    13381 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    13441 cacctggtgc atgacccgca agcccggtgc ctgacacgtg ctacgagatt tcgattccac
    13501 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    13561 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
    13621 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    13681 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
    13741 gtcgacctct agctagagct tggcgtaatc atggtcatta ccaatgctta atcagtgagg
    13801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    13861 agataactac gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag
    13921 aaccacgctc accggctccg gatttatcag caataaacca gccagccgga agggccgagc
    13981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    14041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca
    14101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    14161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    14221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    14281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    14341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    14401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    14461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    14521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    14581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat
    14641 tcttcctt
//
