LOCUS       sequence-495281-       11223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding a chimeric protein composed of the 
            transmembrane region of FCRL3 and its full-length cytoplasmic tail, 
            fused to the extracellular domain of EGFR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Bianchi N, Foli E, Mostanfar M, Marzi R, Spinella MC, Polletti S, 
            Pecoraro M, Cassotta A, Thakur R, Jarrossay D, Sallusto F, Natoli G,
            Monticelli S
  TITLE     FCRL3 is an immunoregulatory receptor that restrains the activation 
            of human memory T lymphocytes
  JOURNAL   J Exp Med (2026) 223 (1): e20242474.
REFERENCE   2  (bases 1 to 11223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med 
            (2026) 223 (1): e20242474."
COMMENT     Sequence Label: pLVX-EF1_-EGFR-FCRL3-140aa-Chimera-puro
FEATURES             Location/Qualifiers
     source          1..11223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    742..857
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(1120..1164)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1349..1582
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2079..2204
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2251..2884
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3052..3186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3076..3095)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3130..3149
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3234..3338
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3339..3407,3408..4199)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3557..3576)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4370..4958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4859..4878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5112..5129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5246..5267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5282..5287,5288..5305,5306..5312)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5320..5336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5325..5347
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5344..5360
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5344..5360
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             5488..6121
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6328..6916
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6434..6445
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6843..6863
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6933..7532)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7016..7036)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7513..7532
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    7540..8112
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     7928..7945
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     promoter        complement(join(10589..11223,1..548))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(10598..11223,1..317)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(10621..10641)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cttgccagaa aaaaagcgtc gcagcaggtc atcaaaaatt ttaaatggct agagacttat
       61 cgaaagcagc gagacaggcg cgaaggtgcc accagattcg cacgcggcgg ccccagcgcc
      121 caagccaggc ctcaactcaa gcacgaggcg aaggggctcc ttaagcgcaa ggcctcgaac
      181 tctcccaccc acttccaacc cgaagctcgg gatcaagaat cacgtactgc agccaggggc
      241 gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa agaggccagg cccgcgggaa
      301 ccacacacgg cacttacctg tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg
      361 gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg gagccagtac
      421 acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt tcaattgccg
      481 acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca ctgacgggca
      541 ccggagcctc acgcatgctc ttctccacct cagtgatgac gagagcgggc gggtgagggg
      601 gcgggaacgc agcgatctct gggttctacg ttagtgggag tttaacgacg gtccctggga
      661 ttccccaagg caggggcgag tccttttgta tgaattactc atcgataaac tggatctctg
      721 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
      781 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
      841 cccccttttc ttttaaaggc gataccgtcg agatccgttc actaatcgaa tggatctgtc
      901 tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt
      961 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
     1021 aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt
     1081 tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa
     1141 ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg
     1201 tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca
     1261 aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat
     1321 gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac
     1381 agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg
     1441 ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg
     1501 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
     1561 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
     1621 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
     1681 ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccggcc
     1741 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     1801 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     1861 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     1921 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
     1981 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
     2041 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
     2101 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
     2161 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
     2221 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
     2281 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     2341 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     2401 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaaagcagc tgcttatatg
     2461 caggatctga gggctcgcca ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc
     2521 ccagcggaaa gtcccttgta gcaagctcga tatcagcagt tcttgaagta ctccggatgc
     2581 agctctcggg ccacgtgatg aaatgctagg cggctgtcaa acctccactc taacacttct
     2641 ctctccgggt catccatccc atgcaggctc acagggtgta acaagctggt gttctctcct
     2701 ttattggcct cttctacctt atctggctca actggtacta gcttgtagca ccatccaaag
     2761 gtcagtggat atctgacccc tggccctggt gtgtagttct gctaatcagg gaagtagcct
     2821 tgtgtgtggt agatccacag atcaaggata tcttgtcttc tttgggagtg aattagccct
     2881 tccaactact aagtttgtag tacatattta acaaatacaa tttctttaaa atgaaaataa
     2941 ttcagaggaa tcacaggttt agagtaaatg aaaccacagg taattggcag tggtaatagg
     3001 gtatggggtg ggaagtttgg gatgattttg gttagcttga gttatccagt tgatccagac
     3061 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     3121 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3181 caagttgatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3241 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3301 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3361 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3421 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3481 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3541 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3601 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3661 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3721 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3781 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3841 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3901 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3961 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4021 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4081 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4141 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4201 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4261 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4321 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4381 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4441 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4501 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4561 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4621 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4681 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4741 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4801 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4861 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4921 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4981 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5041 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5101 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5161 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5221 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     5281 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     5341 acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc gacggtaaac
     5401 gctagcaatg tcaaggcctc tcactctctg atattcattt ctttgcaagt tataaatact
     5461 gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac taaaagggtc
     5521 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     5581 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     5641 ctcagatctg gtctaaccag agagacccag tacaggcaaa aagcagctgc ttatatgcag
     5701 gatctgaggg ctcgccactc cccagtcccg cccaggccac gcctccctgg aaagtcccca
     5761 gcggaaagtc ccttgtagca agctcgatat cagcagttct tgaagtactc cggatgcagc
     5821 tctcgggcca cgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa cacttctctc
     5881 tccgggtcat ccatcccatg caggctcaca gggtgtaaca agctggtgtt ctctccttta
     5941 ttggcctctt ctaccttatc tggctcaact ggtactagct tgtagcacca tccaaaggtc
     6001 agtggatatc tgacccctgg ccctggtgtg tagttctgct aatcagggaa gtagccttgt
     6061 gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat tagcccttcc
     6121 agtcccctct tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt
     6181 cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg tgcttctagc
     6241 caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc atgtttttct
     6301 aggtctcgac tgcagaatta attccaggcg gggaggcggc ccaaagggag atccgactcg
     6361 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     6421 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     6481 aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg
     6541 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     6601 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     6661 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     6721 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     6781 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     6841 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
     6901 aatccagagg ttgattgttc cagagcgatc gctcaggcac cgggcttgcg ggtcatgcac
     6961 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
     7021 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
     7081 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
     7141 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
     7201 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
     7261 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg
     7321 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
     7381 tcttgcagct cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg
     7441 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg
     7501 gcgaggcgca ccgtgggctt gtactcggtc atgctaagct tatcatcgtg tttttcaaag
     7561 gaaaaccacg tccccgtggt tcggggggcc tagacgtttt ttaacctcga ctaaacacat
     7621 gtaaagcatg tgcaccgagg ccccagatca gatcccatac aatggggtac cttctgggca
     7681 tccttcagcc ccttgttgaa tacgcttgag gtgagccatt tgactctttc cacaactatc
     7741 caactcacaa cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc
     7801 ttttggccgc agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct
     7861 acagacgttg tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac
     7921 agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca
     7981 gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga aaataacata
     8041 tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg
     8101 gagggagagg ggcgggatcc gcggccgcct agtggtctga ggccagtaat acacgtggta
     8161 cattctcata gttttcttca tcatcttctt catgggccct gcctctgctg ctagcctccc
     8221 ctgcagagtc gtctgggtgt gtcttcttca gttctgaata gaggactgta agttcctcat
     8281 gctcttgatg catcattgga caattagctg agttttcttt tgtatgctgg atgctccaga
     8341 tctgggaata aatcgggttg ctatctccag gatttacatt gctgtacatt ggctccagct
     8401 ccattggggc tagtggttta gagtgagtgg gctcttgagg gtctatcctg gaaggcctgg
     8461 acgaggaagg ctcctgacac tcactaggac tgtgactaga tgttccagtg gcagaaagtc
     8521 ctcctggttt ccttcgggcc ctggcgtaat gcagcagagc agcagcagca gcaaggacga
     8581 ggatgctgag caccagcccc gtgattcccg cagcggacgg gatcttaggc ccattcgttg
     8641 gacagccttc aagacctggc ccagtgcatc cgtaggtgca gtttggatgg cacaggtggc
     8701 acacatggcc ggcgtctgcg tacttccaga ccagggtgtt gttttctccc atgactcctg
     8761 ccgggcaggt cttgacgcag tgggggccgt caatgtagtg ggcacactgg atacagttgt
     8821 ctggtccccg tcctgtgcag gtgatgttca tggcctgagg caggcactct gggtggcact
     8881 gtatgcactc agagttctcc acaaactccc ttggctcacc ctccagaagg ttgcacttgt
     8941 ccacgcattc cctgcctcgg ctgacattcc ggcaagagac gcagtccctg ggctccgggc
     9001 cccagcagcc ctcgggggag cacaaggcat ggcagacctg gcctgtggcc ttgcagctgt
     9061 tttcacctct gttgcttata attttggttt tctgaccgga ggtcccaaac agttttttcc
     9121 agtttattgt atttgcatag cacaaatttt tgtttcctga aattatcaca tctccatcac
     9181 ttatctcctt gagggagcgt aatcccaagg atgttatgtt caggctgacg actgcaagag
     9241 aaaactgacc atgttgcttg gtcctgccgc gtatgatttc taggttctca aaggcatgga
     9301 ggtccgtcct gttttcaggc caagcctgaa tcagcaaaaa ccctgtgatt tcctttacgg
     9361 ttttcagaat atccagttcc tgtggatcca gaggaggagt atgtgtgaag gagtcacccc
     9421 taaatgccac cggcaggatg tggagatcgc cactgatgga ggtgcagttt ttgaagtgtt
     9481 taatattcgt agcatttatg gagagtgagt ctttaaattc accaatacct attccgttac
     9541 acactttgcg gcaaggccct tcgcacttct tacacttgcg gacgccgtct tcctccatct
     9601 catagctgtc ggccccacag gctcggacgc acgagccgtg atctgtcacc acataattac
     9661 ggggacactt cttcacgcag gtggcaccaa agctgtattt gccctcgggg ttcacatcca
     9721 tctggtacgt ggtggggttg tagagcatga gtggggggca ggtgtccttg cacgtggctt
     9781 cgtctcggaa tttgcggcag accaggcagt cgctctcccg ggggcctgtg cagcctgcag
     9841 cacactggtt gtggcagcag tcactggggg acttgccacg gcagcgcccg gagcactgct
     9901 gggcacagat gattttggtc agtttctggc agttctcctc tcctgcaccc cagcagctcc
     9961 cattgggaca gcttggatca cacttttggc agctgcccag gtggttctgg aagtccatcg
    10021 acatgttgct gagaaagtca ctgctgacta tgtcccgcca ctggatgctc tccacgttgc
    10081 acagggcagg gttgttgctg aaccgcacgg cgccatgcag gatttcctgt aaatttctca
    10141 tgggcagctc cttcagtccg gttttatttg catcatagtt agataagact gctaaggcat
    10201 aggaattttc gtagtacata tttcctctga tgatctgcag gttttccaaa ggaattcgct
    10261 ccactgtgtt gagggcaatg aggacataac cagccacctc ctggatggtc tttaagaagg
    10321 aaagatcata attcctctgc acataggtaa tttccaaatt cccaaggacc acctcacagt
    10381 tattgaacat cctctggagg ctgagaaaat gatcttcaaa agtgcccaac tgcgtgagct
    10441 tgttactcgt gccttggcaa actttctttt cctccagagc ccgactcgcc gggcagagcg
    10501 cagccagcag cgccaggagc gctgccccgg ccgtcccgga gggtcgcatt ctagaactag
    10561 tctcgaggaa ttcaccggaa atagatcctc acgacacctg aaatggaaga aaaaaacttt
    10621 gaaccactgt ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg gcaaattcca
    10681 aggagaatta catcaagtgc caagctggcc taacttcagt ctccacccac tcagtgtggg
    10741 gaaactccat cgcataaaac ccctcccccc aacctaaaga cgacgtactc caaaagctcg
    10801 agaactaatc gaggtgcctg gacggcgccc ggtactccgt ggagtcacat gaagcgacgg
    10861 ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc acccgcccgc tctcccgagc
    10921 gccgcgtcct ccattttgag ctccctgcag cagggccggg aagcggccat ctttccgctc
    10981 acgcaactgg tgccgaccgg gccagccttg ccgcccaggg cggggcgata cacggcggcg
    11041 cgaggccagg caccagagca ggccggccag cttgagacta cccccgtccg attctcggtg
    11101 gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg ggacgcacgg gccccgtcgc
    11161 cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc ttggcccgca tttacaagac
    11221 tat
//
