LOCUS       sequence-495361-        2337 bp    DNA     circular SYN 16-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30016A
FEATURES             Location/Qualifiers
     source          1..2337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..54
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             58..99
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(178..195)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(349..937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(429..448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1108..1899)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2121..2225)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2293..2311
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tgggtaagcc aatccctaat cctctcttgg gtttggattc tactggtgga
       61 aaacctattc caaacccatt gctcggactc gactcaacaa tgtgagacca cgagtgatta
      121 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      181 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      241 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      301 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      541 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      781 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      841 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      901 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1021 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1081 tatatatgag taaacttggt ctgacagtta tttgccgact accttggtga tctcgccttt
     1141 tacgtaatgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
     1201 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
     1261 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
     1321 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
     1381 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
     1441 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
     1501 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
     1561 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
     1621 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
     1681 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
     1741 cgtaaccagc aaatcaatat cgctgtgtgg cttcaggccg ccatccactg cggagccgta
     1801 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
     1861 tagttgagtc gatacttcgg cgatcaccgc ttccctcatg atgtttaact ttgttttagg
     1921 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     1981 gtaacgcgct tgctgcttgg atgcccgagg catagactgt accccaaaaa aaaacagtca
     2041 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     2101 gaccagttgc gtgagcgcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2161 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2221 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     2281 ttaacctata aaaataggcg tatcacgagg ccctttcgtc cactgaagag ccactgt
//
