LOCUS       sequence-495404-       12531 bp    DNA     circular SYN 16-DEC-2025
DEFINITION  Lentiviral expression of hTERT, used to create immortalized cell 
            lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, 
            Betzig E, Meyer T
  TITLE     Engulfed cadherin fingers are polarized junctional structures 
            between collectively migrating endothelial cells.
  JOURNAL   Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
            2016 Nov 14.
  PUBMED    27842057
REFERENCE   2  (bases 1 to 12531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3438"; 
            journalName: "Nat Cell Biol"; date: "2016-12"; volume: "18"; issue: 
            "12"; pages: "1311-1323"
COMMENT     Sequence Label: pLV-hTERT-IRES-hygro
FEATURES             Location/Qualifiers
     source          1..12531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1119..1752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1959..2547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2065..2076
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2474..2494
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2571..3596)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2571..3596)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    3599..4148
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3758..3777)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3987..4004
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(4262..7660)
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     intron          7676..8614
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7699..7719)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(8615..8826)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    8904..9019
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(9282..9326)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9511..9744
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10241..10366
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10413..11046
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    11214..11348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11238..11257)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11292..11311
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        11396..11500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11501..11569,11570..12361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11719..11738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgc cccgcccggg ctattccttt
     2581 gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca
     2641 tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg
     2701 gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca
     2761 accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc
     2821 gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc
     2881 aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc
     2941 tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg
     3001 aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca
     3061 tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac
     3121 acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct
     3181 tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc
     3241 atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac
     3301 gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa ttccccaatg
     3361 tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct
     3421 ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg
     3481 aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg
     3541 aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatggta
     3601 ttatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt
     3661 taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca
     3721 atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt
     3781 gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg
     3841 tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg
     3901 ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc
     3961 ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg
     4021 aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa
     4081 tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc
     4141 agtaacgtta gggggggggg gcggaattga tcccgctaga gggaaaccgt tgctagctta
     4201 agttagttaa caattgttac cgcggcccgt cgactctaga gcggccgccc tcgaggaatt
     4261 ctcagtccag gatggtcttg aagtctgagg gcagtgccgg gttggctgcg gcctccaggg
     4321 cagtcagcgt cgtccccggg agcttccgac tcagctgcgt ctgggctgtc ctgagtgacc
     4381 ccaggagtgg cacgtaggtg acacggtgtc gagtcagctt gagcaggaat gcttggtggc
     4441 acagccactg cacggcctcg gagggcagag ggccggcggc gcccttggcc cccagcgaca
     4501 tccctgcgtt cttggctttc aggatggagt agcagaggga ggccgtgtca gagatgacgc
     4561 gcaggaaaaa tgtggggttc ttccaaactt gctgatgaaa tgggagctgc agcacacatg
     4621 cgtgaaacct gtacgcctgc agcaggagga tcttgtagat gttggtgcac accgtctgga
     4681 ggctgttcac ctgcaaatcc agaaacaggc tgtgacactt cagccgcaag accccaaaga
     4741 gtttgcgacg catgttcctc ccagccttga agccgcggtt gaaggtgaga ctggctctga
     4801 tggaggtccg ggcatagctg gagtagtcgc tctgcacctc cagggtccgg gtatccagca
     4861 gcaggccgca ccaggggaat aggccgtggg ccggcatctg aacaaaagcc gtgccaccca
     4921 gggcctcgtc ttctacaggg aagttcacca ctgtcttccg caagttcacc acgcagccat
     4981 actcagggac acctcggacc agggtcctga ggaaggtttt cgcgtgggtg aggtgaggtg
     5041 tcaccaacaa gaaatcatcc accaaacgca ggagcagccc gtcccgccga atccccgcaa
     5101 acagcttgtt ctccatgtcg ccgtagcaca ggctgcagag cagcgtggag aggatggagc
     5161 cctgcgggat cccctggcac tggacgtagg acttgcccct gatgcgcacg gcgtggtggc
     5221 acatgaagcg taggaagacg tcgaagaggc cactgctggc ctcattcagg gaggagctct
     5281 gctcgatgac gacggcatcc ctcagcgggc tggtctcctg caggtgagcc acgaactgtc
     5341 gcatgtacgg ctggaggtct gtcaaggtag agacgtggct cttgaaggcc ttgcggacgt
     5401 gcccatgggc ggccttctgg accacggcat accgacgcac gcagtacgtg ttctggggtt
     5461 tgatgatgct ggcgatgacc tccgtgagcc tgtcctgggg gatggtgtcg tacgcgcccg
     5521 tcacatccac cttgacaaag tacagctcag gcggcgggtc ctgggcccgc acacgcagca
     5581 cgaaggtgcg ccaggccctg tggatatcgt ccaggcccag cacagaggcg cccaggaggc
     5641 cggggcgccg cgcccgctcg tagttgagca cgctgaacag tgccttcacc ctcgaggtga
     5701 gacgctcggc cctcttttct ctgcggaacg ttctggctcc cacgacgtag tccatgttca
     5761 caatcggccg cagcccgtca ggcttgggga tgaagcggag tctggacgtc agcagggcgg
     5821 gcctggcttc ccgatgctgc ctgacctctg cttccgacag ctcccgcagc tgcaccctct
     5881 tcaagtgctg tctgattcca atgctttgca acttgctcca gacactcttc cggtagaaaa
     5941 agagcctgtt cttttgaaac gtggtctccg tgacataaaa gaaagacctg agcagctcga
     6001 cgacgtacac actcatcagc cagtgcagga acttggccag gatctcctca cgcagacggt
     6061 gctctgcggc cggaacacag ccaacccctg ggctcctgcg cagccaagcg cagccccgca
     6121 cgctcatctt ccacgtcagc tcctgcagcg agagcttggc atgcttcccc agggagatga
     6181 acttcttggt gttcctgagg aagcggcgtt cgttgtgcct ggagccccag aggcctgggg
     6241 gcaccagccg gcgcaggcag gcccgcacga agccgtacac ctgccagggg ctgctgtgct
     6301 ggcggagcag ctgcaccagg cgacgggggt ctgtgtcctc ctcctcgggg gccgccacag
     6361 agccctgggg cttctcccgg gcacagacac cggctgctgg ggtgaccgca gctcgcagcg
     6421 ggcagtgcgt cttgaggagc accccgtagg ggcactgcgc gtggttccca agcagctcca
     6481 gaaacagggg ccgcatttgc cagtagcgct ggggcaggcg gggcaacctg cggggagtcc
     6541 ctggcatcca gggcctggaa cccagaaaga tggtctccac gagcctccga gcgccagtca
     6601 ggctgggcct cagagagctg agtaggaagg agggccgcag ctgctccttg tcgcctgagg
     6661 agtagaggaa gtgcttggtc tcggcgtaca ccgggggaca aggcgtgtcc cagggacgtg
     6721 gtggccgcga tgtggatggg gggcccgcgt ggtgctggcg gcccacggat gggtgggagt
     6781 ggcgcgtgcc agagagcgca ccctccaaag aggtggcttc ttcggcgggt ctggcaggtg
     6841 acaccacaca gaaaccacgg tcactcggtc cacgcgtcct gcccgggtgg gcccaggacc
     6901 cctgcccaac gggcgtccgc tccggctcag gggcagcgcc acgcctgggc ctcttgggca
     6961 acggcagact tcggctggca ctgcccccgc gcctcctcgc acccggggct ggcaggccca
     7021 gggggacccc ggcctccctg acgctatggt tccaggcccg ttcgcatccc agacgccttc
     7081 ggggtccact agcgtgtggc gggggccggg cctgagtggc agcgccgagc tggtacagcg
     7141 gcggcccgca cacctggtag gcgcagctgg gagccaccag cacaaagagc gcgcagcgtg
     7201 ccagcaggtg aaccagcacg tcgtcgccca cgcggcgcag cagcagcccc cacgccccgc
     7261 tcccccgcag tgcgtcggtc accgtgttgg gcaggtagct gcgcacgctg gtggtgaagg
     7321 cctcgggggg gcccccgcgg gccccgtcca gcagcgcgaa gccgaaggcc agcacgttct
     7381 tcgcgccgcg ctcgcacagc ctctgcagca ctcgggccac cagctccttc aggcaggaca
     7441 cctggcggaa ggagggggcg gcggggggcg gccgtgcgtc ccagggcacg cacaccaggc
     7501 actgggccac cagcgcgcgg aaagccgccg ggtccccgcg ctgcaccagc cgccagccct
     7561 ggggccccag gcgccgcacg aacgtggcca gcggcagcac ctcgcggtag tggctgcgca
     7621 gcagggagcg cacggctcgg cagcggggag cgcgcggcat ggatcctcac gacacctgaa
     7681 atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact
     7741 caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct
     7801 ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg
     7861 acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg
     7921 agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac
     7981 ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa
     8041 gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg
     8101 gggcgataca cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc
     8161 cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg
     8221 acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt
     8281 ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat
     8341 tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc
     8401 gcacgcggcg gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc
     8461 cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa
     8521 tcacgtactg cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga
     8581 ggccaggccc gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc
     8641 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
     8701 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
     8761 caccggttca attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct
     8821 ctgcccactg acgggcacct cgagtgaaga cgaaagggcc tcatcgataa actggatctc
     8881 tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt
     8941 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
     9001 tccccccttt tcttttaaag gcgataccgt cgagatccgt tcactaatcg aatggatctg
     9061 tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg
     9121 gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata
     9181 gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat
     9241 tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct
     9301 aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag
     9361 tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc
     9421 caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa
     9481 atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc
     9541 acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc
     9601 tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat
     9661 tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc
     9721 aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt
     9781 ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat
     9841 aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgg
     9901 ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt
     9961 ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct actgtattat
    10021 ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt
    10081 gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc
    10141 tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa
    10201 ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc
    10261 tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc
    10321 ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc
    10381 ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa
    10441 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
    10501 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
    10561 ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata
    10621 tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc cctggaaagt
    10681 ccccagcgga aagtcccttg tagcaagctc gatatcagca gttcttgaag tactccggat
    10741 gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc aaacctccac tctaacactt
    10801 ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagctg gtgttctctc
    10861 ctttattggc ctcttctacc ttatctggct caactggtac tagcttgtag caccatccaa
    10921 aggtcagtgg atatctgacc cctggccctg gtgtgtagtt ctgctaatca gggaagtagc
    10981 cttgtgtgtg gtagatccac agatcaagga tatcttgtct tctttgggag tgaattagcc
    11041 cttccaacta ctaagtttgt agtacatatt taacaaatac aatttcttta aaatgaaaat
    11101 aattcagagg aatcacaggt ttagagtaaa tgaaaccaca ggtaattggc agtggtaata
    11161 gggtatgggg tgggaagttt gggatgattt tggttagctt gagttatcca gttgatccag
    11221 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
    11281 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
    11341 aacaagttga tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg
    11401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    11461 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    11521 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    11581 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    11641 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    11701 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    11761 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    11821 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    11881 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    11941 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    12001 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    12061 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    12121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    12181 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    12241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    12301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    12361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    12421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    12481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
