LOCUS       sequence-495479-        2394 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2394)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30051
FEATURES             Location/Qualifiers
     source          1..2394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             88..117
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             127..150
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(235..252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(406..994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(486..505)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1165..1956)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2178..2282)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2350..2368
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggatgtatt caagaaagga ttttcaatag ccgatgaggg agttgtggga
       61 gctgttgaga gcagcggtag cggtgcacat catcaccacc atcaccatca tcaccatagc
      121 agcggtctgg aagttctgtt tcagggccca ggagcaatgt gagaccacga gtgattaatg
      181 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      241 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      301 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      361 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      421 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      481 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      541 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      601 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      661 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      721 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      781 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      841 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      901 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      961 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1021 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1081 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1141 atatgagtaa acttggtctg acagttattt gccgactacc ttggtgatct cgccttttac
     1201 gtaatggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
     1261 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
     1321 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
     1381 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     1441 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     1501 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     1561 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
     1621 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     1681 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     1741 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
     1801 aaccagcaaa tcaatatcgc tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     1861 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     1921 ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg
     1981 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     2041 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaaa acagtcataa
     2101 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     2161 cagttgcgtg agcgcatact cttccttttt caatattatt gaagcattta tcagggttat
     2221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2281 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2341 acctataaaa ataggcgtat cacgaggccc tttcgtccac tgaagagcca ctgt
//
