LOCUS       sequence-495538-        2463 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2463)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30026
FEATURES             Location/Qualifiers
     source          1..2463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             31..54
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             91..114
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             118..135
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             136..159
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             160..225
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(304..321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(475..1063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(555..574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1234..2025)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2247..2351)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2419..2437
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggatagctt ggcgcgccaa tggagccacc cgcagttcga aaaaggcgga
       61 ggttccggcg gaggttctgg tggaggctcc tggagccacc cgcagttcga aaaaggccat
      121 caccatcacc atcacctgga agttctgttc caggggccag actacaaaga ccatgacggt
      181 gattataaag atcatgatat cgattacaag gatgacgatg acaaaatgtg agaccacgag
      241 tgattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      301 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      361 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      421 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      481 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      541 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      601 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      661 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      721 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      781 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      841 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      901 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      961 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1021 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1081 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1201 ctaaagtata tatgagtaaa cttggtctga cagttatttg ccgactacct tggtgatctc
     1261 gccttttacg taatggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     1321 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     1381 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     1441 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     1501 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     1561 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     1621 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     1681 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     1741 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     1801 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     1861 ggtcaccgta accagcaaat caatatcgct gtgtggcttc aggccgccat ccactgcgga
     1921 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     1981 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt
     2041 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     2101 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaaa
     2161 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     2221 gttctggacc agttgcgtga gcgcatactc ttcctttttc aatattattg aagcatttat
     2281 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2341 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2401 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtccact gaagagccac
     2461 tgt
//
