LOCUS       sequence-495570-        2524 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2524)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50002
FEATURES             Location/Qualifiers
     source          1..2524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             33..284
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     221..242
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(324..342)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        410..514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             736..1527
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1698..2286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2187..2206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2440..2457
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gaacgcggct attagatcaa tggacaagca gaagaacggc atcaaggcca
       61 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
      121 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
      181 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      241 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgagct ttgagaccac
      301 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      361 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      421 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      481 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      541 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      601 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      661 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      721 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      781 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      841 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      901 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      961 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     1021 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     1081 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     1141 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     1201 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     1261 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1321 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1381 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1441 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1501 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1561 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1621 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1681 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1741 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1801 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1861 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1921 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1981 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2041 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2101 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2161 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2221 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2281 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2341 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2401 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2461 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2521 ctgt
//
