LOCUS       sequence-495589-       11612 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Binary vector to receive a NotI fragment from pART7 with a gene to 
            be stably expressed in plants via agrobacterium-mediated 
            transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Gleave AP
  TITLE     A versatile binary vector system with a T-DNA organisational 
            structure conducive to efficient integration of cloned DNA into the 
            plant genome.
  JOURNAL   Plant Mol Biol. 1992 Dec;20(6):1203-7.
  PUBMED    1463857
REFERENCE   2  (bases 1 to 11612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "1992-12"; volume: "20(6)"; pages: "1203-7"
COMMENT     Sequence Label: pART27
FEATURES             Location/Qualifiers
     source          1..11612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..58)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     649..668
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1404..1656
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1719..1743
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     oriT            2298..2407
                     /note="incP origin of transfer"
     mobile_element  2467..3234
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             3859..5007
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     primer_bind     complement(6196..6213)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6367..6955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6447..6466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8052..8840
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9515..10150
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    10638..10662
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    10917..10938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10953..10958,10959..10976,10977..10983)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10991..11007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10996..11018
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11015..11031
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11015..11031
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             11027..11377
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYRPAGGRTSDIPRPWRPG
                     ACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS
                     QQLRSLNGEWKL"
     promoter        11049..11067
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     11049..11066
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      11147..11156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(11186..11205)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11187..11205)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11212..11229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11212..11228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11221..11243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        11386..11569
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             join(11570..11611,11612,1..8,9..779)
                     /codon_start=1
                     /gene="nos-nptII"
                     /product="fusion between an N-terminal peptide of nopaline 
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ggatccgggc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
       61 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
      121 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
      181 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
      241 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
      301 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
      361 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
      421 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
      481 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
      541 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
      601 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
      661 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
      721 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
      781 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
      841 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
      901 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccgatccaac
      961 acttacgttt gcaacgtcca agagcaaata gaccacgaac gccggaaggt tgccgcagcg
     1021 tgtggattgc gtctcaattc tctcttgcag gaatgcaatg atgaatatga tactgactat
     1081 gaaactttga gggaatactg cctagcaccg tcacctcata acgtgcatca tgcatgccct
     1141 gacaacatgg aacatcgcta tttttctgaa gaattatgct cgttggagga tgtcgcggca
     1201 attgcagcta ttgccaaaat cgaaataccc ctcacgcatg cattcatcaa tattattcat
     1261 gcggggaaag gcaagattaa tccaactggc aaatcatcca gcgtgattgg taacttcagt
     1321 tccagcgact tgattcgttt tggtgctacc cacgttttca ataaggacga gatggtggag
     1381 taaagaagga gtgcgtcgaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat
     1441 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     1501 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     1561 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     1621 aattatcgcg cgcggtgtca tctatgttac tagatcgaat taattcagta cattaaaaac
     1681 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     1741 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     1801 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     1861 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     1921 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     1981 aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa
     2041 ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag
     2101 ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga tcgtcgacgg
     2161 atcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     2221 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     2281 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     2341 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     2401 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
     2461 accaggggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc
     2521 agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa
     2581 cggcaaaagc accgccggac atcagcgcta tctctgctct cactgccgta aaacatggca
     2641 actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc
     2701 catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc tcaacacgat
     2761 tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt
     2821 gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc taaatcgcgc
     2881 cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc gcacgtattc
     2941 ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg
     3001 gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac
     3061 gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac
     3121 ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa
     3181 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat tacccaacca
     3241 ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga gcgattgagg
     3301 aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc ggccagggct
     3361 acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc atcaatggcg
     3421 acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg cgcacggcgc
     3481 ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag caggacgagc
     3541 ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt ttttagccgc
     3601 taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc catcaagaag
     3661 agcgacttcg cggagctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag
     3721 gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc
     3781 aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca
     3841 atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg
     3901 atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt
     3961 gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc
     4021 gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag
     4081 cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg
     4141 cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc
     4201 agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag
     4261 ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg
     4321 gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac
     4381 aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg
     4441 gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc
     4501 ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat
     4561 ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc
     4621 ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc
     4681 ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg
     4741 ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg
     4801 acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg
     4861 gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag
     4921 gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat
     4981 gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca
     5041 gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg cacttgcttc
     5101 gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac agaggattaa
     5161 aattgacaat tgtgattaag gctcagattc gacggcttgg agcggccgac gtgcaggatt
     5221 tccgcgagat ccgattgtcg gccctgaaga aagctccaga gatgttcggg tccgtttacg
     5281 agcacgagga gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat gccgtggcat
     5341 tcggcgccta catcgacggc gagatcattg ggctgtcggt cttcaaacag gaggacggcc
     5401 ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa cagcgaggcc
     5461 gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg
     5521 tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata
     5581 tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg
     5641 cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta
     5701 gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt
     5761 tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg
     5821 cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc
     5881 tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag
     5941 tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg
     6001 gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac
     6061 ctacagttgt ttccttactg ggctttctca gccgggatgg cgctaagaag ctattgccgc
     6121 cgatcttcat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     6181 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6241 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6301 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6361 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6421 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6481 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6541 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6601 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6661 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6721 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6781 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6841 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6901 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat atcaagaaga
     6961 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7021 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7081 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7141 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7201 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7261 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7321 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaaacaagt
     7381 ggcagcaacg gattcgcaaa cctgtcacgc cttttgtgcc aaaagccgcg ccaggtttgc
     7441 gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct gagcaggtgg
     7501 cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat gtgcacctat
     7561 ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag gattacatct
     7621 cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac caagagcttg
     7681 tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa cacattgttg
     7741 tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt
     7801 gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac aatgtacctg
     7861 cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg ttcacgtata
     7921 aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc tcgggagcac
     7981 aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg
     8041 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     8101 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     8161 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     8221 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     8281 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     8341 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     8401 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     8461 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     8521 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     8581 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     8641 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     8701 cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga caagaagatc
     8761 gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa ggcgagatca
     8821 ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc
     8881 ggcttaactc aagcgttaga gagctgggga agactatgcg cgatctgttg aaggtggttc
     8941 taagcctcgt acttgcgatg gcatcggggc aggcacttgc tgacctgcca attgttttag
     9001 tggatgaagc tcgtcttccc tatgactact ccccatccaa ctacgacatt tctccaagca
     9061 actacgacaa ctccataagc aattacgaca atagtccatc aaattacgac aactctgaga
     9121 gcaactacga taatagttca tccaattacg acaatagtcg caacggaaat cgtaggctta
     9181 tatatagcgc aaatgggtct cgcactttcg ccggctacta cgtcattgcc aacaatggga
     9241 caacgaactt cttttccaca tctggcaaaa ggatgttcta caccccaaaa ggggggcgcg
     9301 gcgtctatgg cggcaaagat gggagcttct gcggggcatt ggtcgtcata aatggccaat
     9361 tttcgcttgc cctgacagat aacggcctga agatcatgta tctaagcaac tagcctgctc
     9421 tctaataaaa tgttaggagc ttggctgcca tttttggggt gaggccgttc gcggccgagg
     9481 ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg
     9541 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca
     9601 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac
     9661 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat
     9721 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat
     9781 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc
     9841 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc
     9901 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt
     9961 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc
    10021 gcgaggtatc cacaacgccg gcggccggcc gcggtgtctc gcacacggct tcgacggcgt
    10081 ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc
    10141 ggtgagcgtc ggaaagggtc gacgatcttg ctgcgttcgg atattttcgt ggagttcccg
    10201 ccacagaccc ggattgaagg cgagatccag caactcgcgc cagatcatcc tgtgacggaa
    10261 ctttggcgcg tgatgactgg ccaggacgtc ggccgaaaga gcgacaagca gatcacgctt
    10321 ttcgacagcg tcggatttgc gatcgaggat ttttcggcgc tgcgctacgt ccgcgaccgc
    10381 gttgagggat caagccacag cagcccactc gaccttctag ccgacccaga cgagccaagg
    10441 gatctttttg gaatgctgct ccgtcgtcag gctttccgac gtttgggtgg ttgaacagaa
    10501 gtcattatcg cacggaatgc caagcactcc cgaggggaac cctgtggttg gcatgcacat
    10561 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
    10621 acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga tagtttaaac
    10681 tgaaggcggg aaacgacaat ctgatcatga gcggagaatt aagggagtca cgttatgacc
    10741 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga
    10801 aggagccact cagccccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10861 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    10921 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    10981 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
    11041 gccaagctat ttaggtgaca ctatagaata ctcaagctat gcatccaacg cgttgggagc
    11101 tctcccatat cgacctgcag gcggccgcac tagtgatatc ccgcggccat ggcggccggg
    11161 agcatgcgac gtcgggccca attcgcccta tagtgagtcg tattacaatt cactggccgt
    11221 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    11281 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    11341 acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatgggtt tctggagttt
    11401 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca
    11461 ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct
    11521 cggtatccaa ttagagtctc atattcactc tcaatccaaa taatctgcaa tggcaattac
    11581 cttatccgca acttctttac ctatttccgc cc
//
