LOCUS       sequence-495712-       13861 bp    DNA     circular SYN 18-DEC-2025
DEFINITION  CRISPR Editing with SpCas9, with miRNA-124 target sequence to 
            repress transgene expression in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13861)
  AUTHORS   Deglon Lab
  TITLE     Deglon Lab Plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: SIN-cPPT-G1B3-spCas9-WPRE-miR124T
FEATURES             Location/Qualifiers
     source          1..13861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3344..3368
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3374..7477
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3374..7477
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3374..7477
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7490..7510
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(7554..7578)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7601..7617)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7602..7618)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    7631..8219
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7684..7704)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8102..8113)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8423..8656
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8681..8699)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8682..8699)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8989..9008)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9222..9241)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9341..9363
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9401..9419)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9487..9591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9592..9660,9661..10452)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10623..11211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11112..11131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11365..11382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11452..11472)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11457..11786
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11637..11772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12920..12985
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13115..13135
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13560..13694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13597..13616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13651..13670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatccg atgtacgggc
     2161 cagatatacg cgttgacatt gattattgac taggatccgg taccgaattc gagctcggta
     2221 ccagatctaa catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc
     2281 ctgagctggc tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac
     2341 ctggcagcat tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca
     2401 ccttggcaca gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc
     2461 ccccctcaaa tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct
     2521 gacagcctgg catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt
     2581 ggcgccaccg gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag
     2641 gggatgccca ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca
     2701 gaagtccaag gacacaaatg ggtgagggga gagctctccc catagctggg ctgcggccca
     2761 accccacccc ctcaggctat gccagggggt gttgccaggg gcacccgatc ttcctcttca
     2821 tgcccagtga atgactcacc ttggcacaga cacaatgttc ggggtgggca cagtgcctgc
     2881 ttcccgccgc accccagccc ccctcaaatg ccttccgaga agcccattga gcaggcccga
     2941 tcttcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg
     3001 cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt
     3061 gagcaggccc gatcttcctc ttcatgccca gtgaatgact caccttggca cagacacaat
     3121 gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca gcccccctca aatgccttcc
     3181 gagaagccca ttgagcaggc ccgggcatcg ccagtctagc ccactccttc ataaagccct
     3241 cgcatcccag gagcgagcag agccagagca ggttggagag gagacgcatc acctccgctg
     3301 ctcgcgggga tcctagtgga tcccccgggc tgcaggaatt atcacaagtt tgtacaaaaa
     3361 agcaggctcc accatggaca agaagtactc cattgggctc gatatcggca caaacagcgt
     3421 cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg
     3481 caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg
     3541 ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa
     3601 gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc
     3661 tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca
     3721 cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata
     3781 tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct
     3841 cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc
     3901 agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt
     3961 cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct
     4021 gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg
     4081 cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt
     4141 cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga
     4201 caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct
     4261 gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc
     4321 gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa
     4381 ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa
     4441 aaatggctac gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat
     4501 taagcccatc ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga
     4561 agatctgttg cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct
     4621 gggcgaactg cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa
     4681 cagggaaaag attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc
     4741 ccggggaaat tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg
     4801 gaacttcgag gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac
     4861 taactttgat aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga
     4921 gtacttcaca gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa
     4981 gccagcattc ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa
     5041 ccggaaagtt accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga
     5101 ctctgttgaa atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga
     5161 tctcctgaaa atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct
     5221 tgaggacatt gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt
     5281 gaaaacttac gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata
     5341 tacaggatgg gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg
     5401 aaagacaatc ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt
     5461 gatccatgat gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca
     5521 gggggacagt cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg
     5581 aatactgcag accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc
     5641 cgagaatatc gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa
     5701 cagtagggaa aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct
     5761 taaggaacac ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct
     5821 gcagaacggc agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta
     5881 cgacgtggat catatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt
     5941 gttgacaaga tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt
     6001 caagaaaatg aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa
     6061 gttcgataat ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt
     6121 catcaaaagg cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga
     6181 ttcacgcatg aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat
     6241 tactctgaag tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag
     6301 agagatcaac aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc
     6361 acttatcaaa aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta
     6421 cgatgttagg aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta
     6481 cttcttttac agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga
     6541 gattcggaag cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa
     6601 gggtagggat ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa
     6661 aaagaccgaa gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag
     6721 cgacaagctg atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc
     6781 tcctacagtc gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa
     6841 actcaaaagc gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa
     6901 aaaccccatc gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat
     6961 taagcttccc aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag
     7021 tgcgggcgag ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt
     7081 gtatctggcc agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca
     7141 gctgttcgtg gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt
     7201 ctccaaaaga gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa
     7261 gcacagggat aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac
     7321 caacttgggc gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta
     7381 cacctctaca aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta
     7441 tgaaacaaga atcgacctct ctcagctcgg tggagacagc agggctgacc ccaagaagaa
     7501 gaggaaggtg tgaaagggtt cgatccctac cggttagtaa tgagtttatc tagacccagc
     7561 tttcttgtac aaagtggtga taattcgata tcaagcttat cgataccgtc gacctcgagg
     7621 gaattccgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7681 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7741 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7801 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7861 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7921 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7981 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     8041 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8101 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8161 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     8221 tcgggaattc gagctcggta cctaatggca ttcaccgcgt gccttaattc gaatggcatt
     8281 caccgcgtgc cttaaacgcg ttggcattca ccgcgtgcct taaatgcatt ggcattcacc
     8341 gcgtgcctta aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     8401 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     8461 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8521 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8581 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8641 tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
     8701 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
     8761 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
     8821 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
     8881 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
     8941 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     9001 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     9061 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     9121 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     9181 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
     9241 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     9301 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     9361 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     9421 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     9481 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9541 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9601 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9661 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9721 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9781 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9841 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9901 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9961 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    10021 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    10081 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    10141 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    10201 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    10261 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    10321 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    10381 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10441 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10501 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10561 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10621 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10681 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10741 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10801 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10861 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10921 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10981 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    11041 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    11101 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    11161 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    11221 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    11281 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    11341 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    11401 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt
    11461 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    11521 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    11581 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    11641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    11701 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    11761 tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt
    11821 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
    11881 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
    11941 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
    12001 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
    12061 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac
    12121 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
    12181 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
    12241 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
    12301 aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
    12361 ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg
    12421 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
    12481 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
    12541 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
    12601 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa
    12661 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
    12721 ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac
    12781 gatgatttat acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc
    12841 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
    12901 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
    12961 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
    13021 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
    13081 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
    13141 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
    13201 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
    13261 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
    13321 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
    13381 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
    13441 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
    13501 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    13561 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    13621 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    13681 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    13741 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    13801 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    13861 t
//
