LOCUS       sequence-496155-        8332 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  A CRISPR-based technology to study chromosome-specific aneuploidy by
            targeting human centromeres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Bosco N, Goldberg A, Zhao X, Mays JC, Cheng P, Johnson AF, Bianchi 
            JJ, Toscani C, Di Tommaso E, Katsnelson L, Annuar D, Mei S, 
            Faitelson RE, Pesselev IY, Mohamed KS, Mermerian A, 
            Camacho-Hernandez EM, Gionco CA, Manikas J, Tseng YS, Sun Z, Fani S,
            Keegan S, Lippman SM, Fenyo D, Giunta S, Santaguida S, Davoli T
  TITLE     KaryoCreate: A CRISPR-based technology to study chromosome-specific 
            aneuploidy by targeting human centromeres.
  JOURNAL   Cell. 2023 Apr 27;186(9):1985-2001.e19. doi: 
            10.1016/j.cell.2023.03.029. Epub 2023 Apr 18.
  PUBMED    37075754
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.03.029"; journalName: "Cell"; date: 
            "2023-04-27- 27"; volume: "186"; issue: "9"; pages: "1985-2001.e19"
COMMENT     Sequence Label: pLenti-gRNA2-4
FEATURES             Location/Qualifiers
     source          1..8332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(80..91)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             269..502
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    580..701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(617..636)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     671..690
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      741..876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(896..915)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(897..915)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(925..942)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(925..941)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(934..956)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1083..1538
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1170..1189)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1380..1401
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1564..1668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1669..1737,1738..2529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1887..1906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2700..3288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3189..3208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3442..3459
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3576..3597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3612..3617,3618..3635,3636..3642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3650..3666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3655..3677
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3674..3690
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3674..3690
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3709..3729
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3711..3729
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3757..3983
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3984..4164
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4211..4336
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4829..5062
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5247..5291
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5469..5717
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5469..5489
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5640..5659
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    5879..5996
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6135..7313
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6366..7304
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7261..7281
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7326..7925
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7326..7345)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7822..7842
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(7941..8332,1..197)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7994..8014)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
       61 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
      121 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
      181 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
      241 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
      301 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      361 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      421 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      481 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
      541 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
      601 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      661 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
      721 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      781 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      841 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
      901 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
      961 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1021 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1081 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1141 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1201 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1261 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     1321 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     1381 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     1441 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1501 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     1561 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1621 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1681 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1741 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1801 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1861 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1921 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1981 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2041 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2101 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2161 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2221 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2281 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2341 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2401 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2461 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2521 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2581 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2641 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2701 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2761 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2821 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2881 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2941 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3001 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3061 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3121 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3181 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3241 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3301 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3361 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3421 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3481 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3541 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3601 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     3661 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     3721 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     3781 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     3841 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     3901 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     3961 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     4021 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4081 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4141 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     4201 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     4261 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     4321 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     4381 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     4441 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     4501 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     4561 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     4621 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     4681 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     4741 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     4801 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     4861 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     4921 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     4981 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     5041 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     5101 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     5161 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     5221 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     5281 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     5341 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     5401 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     5461 gacccagaga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     5521 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     5581 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     5641 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     5701 ggaaaggacg aaacaccgct tcaacactgt tagttgagtt taagagctat gctggaaaca
     5761 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     5821 tgctttttta agcttggcgt aactagatct tgagacaaat ggcagtattc atccacaatt
     5881 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     5941 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     6001 tttattacag ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa
     6061 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga
     6121 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     6181 ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg
     6241 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     6301 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     6361 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     6421 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
     6481 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     6541 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     6601 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     6661 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     6721 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     6781 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     6841 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     6901 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     6961 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     7021 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     7081 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     7141 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     7201 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     7261 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg
     7321 ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     7381 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     7441 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     7501 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc
     7561 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     7621 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     7681 aggagcccgc gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc
     7741 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     7801 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     7861 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     7921 cctgaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     7981 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     8041 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     8101 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     8161 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     8221 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     8281 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gg
//
