LOCUS       sequence-496183-        5030 bp    DNA     circular SYN 05-JAN-2026
DEFINITION  Cloning vector to transiently express an inserted gene in plant 
            cells under the control of the 35S CaMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Gleave AP
  TITLE     A versatile binary vector system with a T-DNA organisational 
            structure conducive to efficient integration of cloned DNA into the 
            plant genome.
  JOURNAL   Plant Mol Biol. 1992 Dec;20(6):1203-7.
  PUBMED    1463857
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "1992-12"; volume: "20(6)"; pages: "1203-7"
COMMENT     Sequence Label: pART7
FEATURES             Location/Qualifiers
     source          1..5030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..36)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(19..35)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(28..50)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(176..631)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(313..334)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     525..544
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        658..762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(763..831,832..1623)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(981..1000)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1794..2382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2283..2302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2536..2553
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2670..2691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2706..2711,2712..2729,2730..2736)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2744..2760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2749..2771
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2768..2784
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2768..2784
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2814..2833
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2814..2832
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3850..4195
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4138..4158
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      4254..4961
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(5002..5020)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5003..5020)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
       61 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
      181 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
      241 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
      301 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
      361 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
      421 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
      481 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
      541 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
      601 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
      661 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      721 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      781 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      841 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      901 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      961 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1021 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1081 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1141 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1201 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1261 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1321 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1381 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1441 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1501 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     1561 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1621 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1681 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1741 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1801 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1861 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1921 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1981 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2041 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2101 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2161 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2221 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2281 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2341 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2401 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2461 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2521 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     2581 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     2641 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     2701 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     2761 tttcacacag gaaacagcta tgacatgatt acgaatttgg ccaagtcggc ctctaatacg
     2821 actcactata gggagctcgt cgagcggccg ctcgacgaat taattccaat cccacaaaaa
     2881 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
     2941 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
     3001 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
     3061 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
     3121 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
     3181 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
     3241 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
     3301 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
     3361 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
     3421 gcaggtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
     3481 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
     3541 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
     3601 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
     3661 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tgaagactgg
     3721 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
     3781 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagacca
     3841 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
     3901 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
     3961 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
     4021 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4081 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
     4141 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
     4201 gaggaattcg gtaccccggg ttcgaaatcg ataagcttgg atcctctaga gtcctgcttt
     4261 aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg
     4321 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa
     4381 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg
     4441 attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata
     4501 ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta
     4561 caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa
     4621 tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa
     4681 ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct
     4741 tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc
     4801 agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt
     4861 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
     4921 gtccagggcg aattttgcga caacatgtcg aggctcagca ggacctgcag gcatgcaagc
     4981 tagcttacta gtgatgcata ttctatagtg tcacctaaat ctgcggccgc
//
