LOCUS       sequence-49641-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, Garcia KC, 
            Bogyo M
  TITLE     Simplified, enhanced protein purification using an inducible, 
            autoprocessing enzyme tag.
  JOURNAL   PLoS One. 2009 Dec 2;4(12):e8119.
  PUBMED    19956581
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-2"; volume: "4(12)"; pages: "e8119"
COMMENT     Sequence Label: pET22b-CPDBamHI-Leu
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     sig_peptide     complement(690..755)
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agaccttgcg cgtcccagct
       61 tagcgaaact ttgttgtttt ctgccttttg aacccaatcg ccattcgcgt ccttggtatg
      121 cttacgtccc gcctcgtcta cggccagttc agaactacga acagagacat cgacacgaag
      181 accattcgca tccatcgcgt taataaactg atgaccaaag cctttttgct tgtcgtcact
      241 caccaaagaa caaccaacaa tactgatgtg atccggtttg ttgttgatgt tttcggcttg
      301 attaaacgac tgttggaact tggccaattt cacggccaac tcatcggcac tgtaaccact
      361 taagcgagta ttgttagttt ctgagtggtc gcgaccatgc cccaccaact gccaacgtag
      421 ctttccatcc agttttgacg gatcgccata caccacgcga tagttgccgt ctgaatcgag
      481 ctgcaccacc acactgcttt cagcatgttt acctgctaaa ttggctgccg cttttgctac
      541 taccgggtcg ttttccattt gaacgatgat ttgaccgtcg aagcgggttt caccaccatc
      601 tgtcgttggt gtaaccgtaa tcgggcccca gctattaaca ttttgattat ggagtatttt
      661 tccggatccg aattaattcc gatatccatg gccatcgccg gctgggcagc gaggagcagc
      721 agaccagcag cagcggtcgg cagcaggtat ttcatatgta tatctccttc tt
//
