LOCUS       sequence-49647-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, Garcia KC, 
            Bogyo M
  TITLE     Simplified, enhanced protein purification using an inducible, 
            autoprocessing enzyme tag.
  JOURNAL   PLoS One. 2009 Dec 2;4(12):e8119.
  PUBMED    19956581
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-2"; volume: "4(12)"; pages: "e8119"
COMMENT     Sequence Label: pET22b-GFP-CPDSalI
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagaccttgc
       61 gcgtcccagc ttagcgaaac tttgttgttt tctgcctttt gaacccaatc gccattcgcg
      121 tccttggtat gcttacgtcc cgcctcgtct acggccagtt cagaactacg aacagagaca
      181 tcgacacgaa gaccattcgc atccatcgcg ttaataaact gatgaccaaa gcctttttgc
      241 ttgtcgtcac tcaccaaaga acaaccaaca atactgatgt gatccggttt gttgttgatg
      301 ttttcggctt gattaaacga ctgttggaac ttggccaatt tcacggccaa ctcatcggca
      361 ctgtaaccac ttaagcgagt attgttagtt tctgagtggt cgcgaccatg ccccaccaac
      421 tgccaacgta gctttccatc cagttttgac ggatcgccat acaccacgcg atagttgccg
      481 tctgaatcga gctgcaccac cacactgctt tcagcatgtt tacctgctaa attggctgcc
      541 gcttttgcta ctaccgggtc gttttccatt tgaacgatga tttgaccgtc gaagcgggtt
      601 tcaccaccat ctgtcgttgg tgtaaccgta atcgggcccc agctattaac attttgatta
      661 tggagtattt ttccatccgc taatgcgtcg accttgtaca gctcgtccat gccgagagtg
      721 atcccggcgg cggtcacgaa ctccancagg accatgtgat cgcgcttctc gttggggtct
      781 ttgctcaggg cggactgggt gctcaggtag t
//
