LOCUS       sequence-496496-        9835 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  To generate cells expressing Nterm tagged miniTurbo deltaVHL for 
            ProtacID via lentivirus (no NLS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Shrestha S, Maitland MER, Jing L, Duan S, Nie DY, St-Germain J, 
            Kanaris M, Barsyte-Lovejoy D, Arrowsmith CH, Raught B
  TITLE     Characterization of PROTAC specificity and endogenous protein 
            interactomes using ProtacID.
  JOURNAL   Nat Commun. 2025 Aug 29;16(1):8089. doi: 10.1038/s41467-025-63357-7.
  PUBMED    40883309
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-08-29"; volume: "16(1):8089. doi"; pages: " 
            10.1038/s41467-025-63357-7"
COMMENT     Sequence Label: pSTV6-3xFLAG-miniTurbo-VHL54:213
FEATURES             Location/Qualifiers
     source          1..9835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    169..303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(193..212)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     247..266
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        351..455
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(456..524,525..1316)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1487..2075
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1976..1995
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2229..2246
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2363..2384
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2399..2404,2405..2422,2423..2429)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2437..2453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2442..2464
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2461..2477
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2461..2477
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2605..3238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     CDS             3443..3454
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3852..3872
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(3912..3920,3921..3956,3957..3959,3960..3995,
                     3996..3998,3999..4034,4035..4040,4041..4658))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(4659..4712)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4713..5309)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4793..4813)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5290..5309
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5319..5829)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5382..5400)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(5839..5856)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        5903..6217
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    5911..5929
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5947..5965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5982..6000
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6018..6036
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6054..6072
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6089..6107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6125..6143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6190..6214
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6247..6271
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6303..6368
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6411..7181
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    7694..7809
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(8072..8116)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8301..8534
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9031..9156
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(9203..9835,1)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 aactactaag tttgtagtac atatttaaca aatacaattt ctttaaaatg aaaataattc
       61 agaggaatca caggtttaga gtaaatgaaa ccacaggtaa ttggcagtgg taatagggta
      121 tggggtggga agtttgggat gattttggtt agcttgagtt atccagttga tccagacatg
      181 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      241 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      301 gttgatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      361 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      421 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      481 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      541 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      601 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      661 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      721 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      781 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      841 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      901 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
      961 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1021 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1081 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1141 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1201 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     1261 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     1321 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1381 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1441 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1501 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1561 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1621 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1681 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1741 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1801 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1861 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1921 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1981 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2041 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2101 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2161 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2221 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2281 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2341 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2401 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2461 caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac ggtaaacgct
     2521 agcaatgtca aggcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa
     2581 taataagatg acatgaacta ctactgctag agattttcca cactgactaa aagggtctga
     2641 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     2701 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     2761 agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat
     2821 ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg
     2881 gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct
     2941 cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc
     3001 gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg
     3061 gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt
     3121 ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg
     3181 tggtagatcc acagatcaag gatatcttgt cttcgttggg agtgaattag cccttccagt
     3241 cccctctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt
     3301 aaaggtacct ctagtccgga cgccgaggcg aaacaggcgg ggaggcggcc caaagggaga
     3361 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     3421 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     3481 gcagaatcca ggtggcaaca caggcgagca gccatggaaa ggacgtcagc ttccccgaca
     3541 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     3601 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     3661 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     3721 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     3781 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     3841 cagcgtatcc acatagcgta aaaggagcaa catagttaag aatacgatat cttggatcga
     3901 tccttactta gttacccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc
     3961 agcatatcaa ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg
     4021 tcaagggcgt cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg
     4081 attagttcca ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt
     4141 gcttgtctca gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct
     4201 acttgatgct cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga
     4261 gcgtacagtg cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc
     4321 agggtttcgt actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcgcga
     4381 tgtgagagga gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac
     4441 tcgccttcca gggggcagaa gtgggtatga tgcctgtcca gcatctcgat tggcagggca
     4501 tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc
     4561 ttttgagcga gtttccttgt cgtcaggcct tcgataccga caccattgag taattccaga
     4621 gctccgttta tgactttgct cttgtccagt ctagacattg gaccagggtt ttcttcaaca
     4681 tcaccacaag tgaggagaga acctctacct tcggcaccgg gcttgcgggt catgcaccag
     4741 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
     4801 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
     4861 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     4921 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     4981 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     5041 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     5101 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     5161 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     5221 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     5281 aggcgcaccg tgggcttgta ctcggtcatg gtgaattgct ggggagagag gtcggtgatt
     5341 cggtcaacga gggagccgac tgccgacgtg cgctccggag gcttgcagaa tgcggaacac
     5401 cgcgcgggca ggaacagggc ccacactacc gccccacacc ccgcctcccg caccgcccct
     5461 tcccggccgc tgctctcggc gcgccctgct gagcagccgc tattggccac agcccatcgc
     5521 ggtcggcgcg ctgccattgc tccctggcgc tgtccgtctg cgagggtact agtgagacgt
     5581 gcggcttccg tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg
     5641 ggcggagcag gaagcgtcgc cggggggccc acaagggtag cggcgaagat ccgggtgacg
     5701 ctgcgaacgg acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa
     5761 ccacgcccag agcagccgcg tccctgcgca aacccagggc tgccttggaa aaggcgcaac
     5821 cccaaccccg gatccttagt ggtggtggtg gtggtggacc ggacgcgttg agcggccgcg
     5881 tctggccctt tcgtcttcac tagagtttac tccctatcag tgatagagaa cgtatgtcga
     5941 gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca gtgatagaga
     6001 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     6061 cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg tatgtcgagt
     6121 ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggaggccta
     6181 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agaaggatcc gcggcctaag
     6241 ctttagacaa gtttgtacaa aaaagctgaa cgagatacgt atccaccggt actctcgaga
     6301 tggactacaa ggaccacgac ggtgactaca aggaccacga catcgactac aaggacgacg
     6361 acgacaaggg gggaggggga ggatctgggg gagggggagg atctatggcg atcccgctgc
     6421 tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg
     6481 tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt
     6541 gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt
     6601 ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg
     6661 gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca
     6721 aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc
     6781 tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta
     6841 acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg
     6901 aagcagggat taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg
     6961 cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc
     7021 tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga
     7081 ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac
     7141 cctggatggg cggagaaatc agtctgcggt ctgccgaaaa gatgcatatg gaggccgggc
     7201 ggccgcggcc cgtgctgcgc tcggtgaact cgcgcgagcc ctcccaggtc atcttctgca
     7261 atcgcagtcc gcgcgtcgtg ctgcccgtat ggctcaactt cgacggcgag ccgcagccct
     7321 acccaacgct gccgcctggc acgggccgcc gcatccacag ctaccgaggt cacctttggc
     7381 tcttcagaga tgcagggaca cacgatgggc ttctggttaa ccaaactgaa ttatttgtgc
     7441 catctctcaa tgttgacgga cagcctattt ttgccaatat cacactgcca gtgtatactc
     7501 tgaaagagcg atgcctccag gttgtccgga gcctagtcaa gcctgagaat tacaggagac
     7561 tggacatcgt caggtcgctc tacgaagatc tggaagacca cccaaatgtg cagaaagacc
     7621 tggagcggct gacacaggag cgcattgcac atcaacggat gggagattaa gaattcccct
     7681 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     7741 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     7801 tcttttaaag gcgataccgt cgagatccgt tcactaatcg aatggatctg tctctgtctc
     7861 tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt
     7921 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag
     7981 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc
     8041 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata
     8101 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc
     8161 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt
     8221 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc
     8281 cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga
     8341 ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct
     8401 caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct
     8461 gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa
     8521 ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct
     8581 tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc
     8641 tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgg ccgcttgctg
     8701 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     8761 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     8821 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     8881 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     8941 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     9001 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     9061 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     9121 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     9181 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     9241 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     9301 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     9361 atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata tgcaggatct
     9421 gagggctcgc cactccccag tcccgcccag gccacgcctc cctggaaagt ccccagcgga
     9481 aagtcccttg tagcaagctc gatatcagca gttcttgaag tactccggat gcagctctcg
     9541 ggccacgtga tgaaatgcta ggcggctgtc aaacctccac tctaacactt ctctctccgg
     9601 gtcatccatc ccatgcaggc tcacagggtg taacaagctg gtgttctctc ctttattggc
     9661 ctcttctacc ttatctggct caactggtac tagcttgtag caccatccaa aggtcagtgg
     9721 atatctgacc cctggccctg gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg
     9781 gtagatccac agatcaagga tatcttgtct tctttgggag tgaattagcc cttcc
//
