LOCUS       sequence-496736-        9993 bp    DNA     circular SYN 08-JAN-2026
DEFINITION  Expresses an EZH2 mutant for knockout/rescue experiments in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Gail EH, Healy E, Flanigan SF, Jones N, Ng XH, Uckelmann M, Levina 
            V, Zhang Q, Davidovich C
  TITLE     Inseparable RNA binding and chromatin modification activities of a 
            nucleosome-interacting surface in EZH2.
  JOURNAL   Nat Genet. 2024 May 14. doi: 10.1038/s41588-024-01740-8.
  PUBMED    38744974
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 8, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2024 May 14. doi: 10.1038/s41588-024-01740-8."
COMMENT     Sequence Label: pHIV-EGFP.Flag-EZH2-mt1
FEATURES             Location/Qualifiers
     source          1..9993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1218..1241
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3492..3512
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    join(3532..4106,4107..4109,4110..4118)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3699..3716)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3926..3945
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4119..4121,4122..4124,4125..4838)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4119..4121,4122..4124,4125..4835)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4164..4185)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4425..4444)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4772..4793
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4850..4866)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4851..4867)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5376..5556
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5618..6206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5698..5717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6377..7168,7169..7237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7000..7019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7238..7342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7417..7436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7608..7987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7989..8187
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8142..8162
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8205..8385
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8432..8557
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9054..9287
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9472..9516
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9814..9931
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag cggccgctga gttaactatt
     1201 ctagagtacc caccatggac tacaaagacg atgacgacaa gggccagact gggaagaaat
     1261 ctgagaaggg accagtttgt tggcggaagc gtgtaaaatc agagtacatg cgactggctc
     1321 agctcaaggc tttcagacga gctgatgaag taaagagtat gtttagttcc aatcgtcaga
     1381 aaattttgga aagaacggaa atcttaaacc aagaatggaa acagcgaagg atacagcctg
     1441 tgcacatcct gacttctgtg agctcattgc gcgggactag ggagtgttcg gtgaccagtg
     1501 acttggattt tccaacacaa gtcatcccat taaagactct gaatgcagtt gcttcagtac
     1561 ccataatgta ttcttggtca cccctacagc agaattttat ggtggaagat gaaactgttt
     1621 tacataacat tccttatatg ggagatgaag ttttagatca ggatggtact ttcattgaag
     1681 aactaataaa aaattatgat gggaaagtac acggggatag agaatgtggg gctataaatg
     1741 atgaaatttt tgtggagttg gtgaatgccc ttggtcaata taatgatgat gacgatgatg
     1801 atgatggaga cgatcctgaa gaaagagaag aaaagcagaa agatctggag gatcaccgag
     1861 atgataaaga aagccgccca cctcggaaat ttccttctga taaaattttt gaagccattt
     1921 cctcaatgtt tccagataag ggcacagcag aagaactaaa ggaaaaatat aaagaactca
     1981 ccgaacagca gctcccaggc gcacttcctc ctgaatgtac ccccaacata gatggaccaa
     2041 atgctaaatc tgttcagaga gagcaaagct tacactcctt tcatacgctt ttctgtaggc
     2101 gatgttttaa atatgactgc ttcctacatc gtaagtgcaa ttattctttt catgcaacac
     2161 ccaacactta taagcggaag aacacagaaa cagctctaga caacaaacct tgtggaccac
     2221 agtgttacca gcatttggag ggagcaaagg agtttgctgc tgctctcacc gctgagcgga
     2281 taaagacccc accaaaacgt ccaggaggcc gcagaagagg acggcttccc aataacagta
     2341 gcaggcccag cacccccacc attaatgtgc tggaatcaaa ggatacagac agtgataggg
     2401 aagcagggac tgaaacgggg ggagagaaca atgataaaga agaagaagag aagaaagatg
     2461 aaacttcgag ctcctctgaa gcaaattctc ggtgtcaaac accaataaag atgaagccaa
     2521 atattgaacc tcctgagaat gtggagtgga gtggtgctga agcctcaatg tttagagtcc
     2581 tcattggcac ttactatgac aatttctgtg ccattgctag gttaattggg accaaaacat
     2641 gtagacaggt gtatgagttt agagtcaaag aatctagcat catagctcca gctcccgctg
     2701 aggatgtgga tactcctcca aggaaaaaga agaggaaaca ccggttgtgg gctgcacact
     2761 gcagaaagat acagctgaaa aaggacggct cctctaacca tgtttacaac tatcaaccct
     2821 gtgatcatcc acggcagcct tgtgacagtt cgtgcccttg tgtgatagca caaaattttt
     2881 gtgaaaagtt ttgtcaatgt agttcagagt gtcaaaaccg ctttccggga tgccgctgca
     2941 aagcacagtg caacaccaag cagtgcccgt gctacctggc tgtccgagag tgtgaccctg
     3001 acctctgtct tacttgtgga gccgctgacc attgggacag taaaaatgtg tcctgcaaga
     3061 actgcagtat tcagcggggc tccaaaaagc atctattgct ggcaccatct gacgtggcag
     3121 gctgggggat ttttatcaaa gatcctgtgc agaaaaatga attcatctca gaatactgtg
     3181 gagagattat ttctcaagat gaagctgaca gaagagggaa agtgtatgat aaatacatgt
     3241 gcagctttct gttcaacttg aacaatgatt ttgtggtgga tgcaacccgc aagggtaaca
     3301 aaattcgttt tgcaaatcat tcggtaaatc caaactgcta tgcaaaagtt atgatggtta
     3361 acggtgatca caggataggt atttttgcca agagagccat ccagactggc gaagagctgt
     3421 tttttgatta cagatacagc caggctgatg ccctgaagta tgtcggcatc gaaagagaaa
     3481 tggaaatccc tccaaaaaag aagagaaagg tataataggg gctaggatcc gcccctctcc
     3541 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     3601 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     3661 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     3721 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     3781 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     3841 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     3901 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     3961 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt
     4021 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     4081 tttcctttga aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagc
     4141 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     4201 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     4261 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     4321 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     4381 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     4441 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     4501 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     4561 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     4621 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     4681 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     4741 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4801 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcatc gataccgtcg
     4861 acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     4921 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     4981 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5041 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     5101 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     5161 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     5221 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     5281 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     5341 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     5401 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5461 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5521 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa
     5581 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5641 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5701 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5761 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5821 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5881 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5941 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6001 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6061 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6121 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6181 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6241 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6301 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6361 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6421 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6481 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6541 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6601 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6661 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6721 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6781 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6841 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6901 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6961 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7021 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7081 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7141 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7201 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7261 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7321 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     7381 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     7441 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     7501 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     7561 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     7621 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     7681 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     7741 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     7801 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     7861 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     7921 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     7981 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8041 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8101 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     8161 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     8221 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8281 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8341 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8401 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8461 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8521 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8581 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8641 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8701 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8761 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     8821 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8881 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8941 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9001 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9061 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9121 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9181 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9241 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9301 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9361 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9421 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9481 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9541 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9601 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9661 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     9721 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     9781 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     9841 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9901 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     9961 agtttggtta gtaccgggcc cgctctagcg tga
//
