LOCUS       sequence-496751-        9182 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PopuA+20xTetO biosensor cicuit in pNBU2 integrative Bacteroides 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9182)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJB158
FEATURES             Location/Qualifiers
     source          1..9182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1933..2004
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2020..2047
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2585..3208
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2658..2677)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    3373..3391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(3478..3480,3481..3483,3484..4197)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(3523..3544)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      4218..4245
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(6225..7016,7017..7085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6848..6867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8377..8765
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            8936..9045
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1981 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2041 ttctgcgttt atatatgatg tgtaatgaca attatccttc cccaggaaaa ttgtaatagc
     2101 cctgttgaac atataaatct agtttagtta ttgacgccaa atatacggtg tttaagtgaa
     2161 atattcaaac ttattttata tttatctgcc ggtttgttct attttgctga gtgatagggg
     2221 aatattaccc ggaaactttc catatcatta caaataaagc cctctgtatg ccccggattt
     2281 tgaatataat ttgtatgttt aatccgtttt ttgtatcttt gtgccagcaa gatactggaa
     2341 caggctgttt ttatacataa aaatgctatt ttttgctatt cgttgaaagg attatcttat
     2401 caaattctat tcctttttta attactcagt ctgtcaataa tcattttatt aattatttcg
     2461 ggattcattg ttatttgtcc cttatttaaa tcagaacttt atacgatata aagaaagatt
     2521 cctgttatcg tttgttcttg tcttgctgtg tatattattt taataaccta aaccatttta
     2581 ttgtatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
     2641 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
     2701 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
     2761 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
     2821 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
     2881 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
     2941 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
     3001 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
     3061 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
     3121 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
     3181 acaacttaaa tgtgaaagtg ggtcttaatg gccacatcgg ttcctgctag aaggcccatc
     3241 ctgacggatg gccttttggt attcgagcat tacctcatac tttggatttt tgttttgttt
     3301 gcaatggtta atctattgtt aaaatttaaa gtttcacttg aactttcaaa taatgttctt
     3361 atatttgcag tgtccctatc agtgatagag agtaggatca cacaacattt aaaaaataac
     3421 attatgaaag cactcgaaaa gagattcaaa ggtaatatta acaataattt attttcaatg
     3481 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3541 gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg agggcgatgc caccaacggc
     3601 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3661 gtgaccacct taggctacgg cgtggcctgc ttcgcccgct accccgacca catgaagcag
     3721 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catctctttc
     3781 aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3841 aaccgcatcg tgctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3901 ctggagtaca acttcaacag ccacaaggtc tatatcacgg ccgacaagca gaagaacggc
     3961 atcaaggcta acttcaagac ccgccacaac gttgaggacg gcggcgtgca gctcgccgac
     4021 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4081 ctgagccatc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4141 aaggagaggg tgaccgccgc cgggattaca catgacatgg acgagctgta caagtaatag
     4201 cgttcagctc gactgtgaga aggccatcct gacggatggc cttttttttt cgttataact
     4261 agttgcgttc ctagccgcta ctagtgtaat tgcctatctt ccagtgatgg aacagcattt
     4321 gtgcattggc tgcaacaatc agccttactt gtgcctgttc tatttccgaa ccgaccgctt
     4381 gtatgaatgc atcaaaattc gttttctcta cgttggattc cttgttgctc atattgtgat
     4441 gataatttct acaaatatag tcattggtaa ctatctatga aactgtttga tacttttata
     4501 gttgattaaa cttgttcatg gcatttgcct taatatcatc cgctatgtca atgtagggtt
     4561 tcatagcttt gtagtcgctg tgtcccgtcc atttcatgac cacctgtgcc gggattccga
     4621 gagccagcgc attgcagatg aatgtccttt ttcctgcatg ggtactgagc aaagcgtatt
     4681 tgtgtgtgac ttcatcaata cgttcatttc ccttgtagta ggtttcccgt acaggctcgt
     4741 tgatttctgc cagttcgccc agctctttca ggtaatcgtt catcttctgg ttgctgatga
     4801 cgggcagagc catgtaattc tcgaaatgga tgtccttgta tttgtccagt atggctttgc
     4861 tgtatttgtt cagttcaatc gtcaggctgt cggcagtctt gactgtggtt atttcgatgt
     4921 ggtcggactt cacatcgctt cttttcagat tgcgaacatc cgaataccgc aaactcgtaa
     4981 agcagcagaa caggaaaaca tcacgcacac gttccaggta ttgcttatcc ttgggtatct
     5041 ggtagtcttt cagcttgttc agttcatccc aagtcaggaa gattactttt ttcgaggtgg
     5101 ttttcagttt cggtttgaac gtatcgtatg caatgttctg atgatgtcct ttcttgaagc
     5161 tccagcgcag gaaccatttg aggaatccca tttgcttgcc gatggtgctg tttctcatat
     5221 ccttggtgtc acgcaggaag ttgacgtatt cgttcaatcc aaactcgttg aaatagttga
     5281 acgttgcatc ctccttgaac tctttgaggt ggttcctcac tgctgcaaat ttttcatagg
     5341 tggatgccgt ccagttattc tggttaccgc actcttttac aaactcatcg aacacctccc
     5401 aaaagctgac aggggcttct tccggctgtt cttcactggt gtctttcatt ctcatgttga
     5461 aagcttcctt caactgttgg gtcgttggca tgacctcctg cacctcaaat tccttgaaaa
     5521 tattctggat ttcggcatag tatttcagca agtccgtatt gatttcggct gcactttgct
     5581 ttagcttgtt ggtacatccg ttctttaccc gctgcttatc tgcatcccat ttggctacgt
     5641 caatccggta gcccgttgta aactcgatac gttggctggc aaagatgaca cgcatacgga
     5701 tgggtacgtt ctctacgatt ggcacaccgt tctttttccg gctctccaat gcaaaaatga
     5761 tgttgcgctt gatattcata attgggtgcg tttgaaattc tacacccaaa tatacaccca
     5821 attattgaga tagcaaaaga catttagaaa catttacttt tactctatat tgtaatttac
     5881 acttgattat cagtcgtttg cagttttatg atattctgtg aaagtataag ttcgagagcc
     5941 tgtctctccg caaaaaacgc tgaaaatcag cagattgcaa aacaaacacc ctgttttaca
     6001 cccaagaatg taaagtcggg tgtttttgtt ttatttaaga taatacaacc actacataat
     6061 aaaagagtag cgatattaaa agaatccgat gagaaaagac taatatttat ctatccattc
     6121 agtttgattt ctcaggactt tacatcgtcc tgaaagtatt tgttggtacc ggtaccgagg
     6181 acgcgacagc tgcagacgcg ttatgagtaa acttggtctg acagttacca atgcttaatc
     6241 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6301 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6361 ccgcgggacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6421 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6481 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6541 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6601 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6661 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6721 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6781 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6841 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6901 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6961 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7021 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7081 ctcatactct tcctttttca atcatggccg cgggattaaa agtcggggat tggtgaacaa
     7141 aaaggtgttt ctctctttaa gagaaatatc gttttgctaa acagttgata ttgaggtatc
     7201 attttatcgt aaaagacatt tttgctcaac aattgcttga cggaaatcaa caaattttag
     7261 cattttgtaa aaaagtcgct atataatttg gtgaattgga gttattttca tatttttgca
     7321 tcccgaagag tttctcttaa agagagaaac atcttttgca taccttttcc gaccgaattt
     7381 ttatgtcgta aagaggggct ttgcaggggg tggactcaga aagatgagaa tagatgacta
     7441 ttgtagttga aacacataga aagttgctga tatacagacc gatacgcata tcgggatgaa
     7501 ccatgagtac gttcttttct caaaaaacat aaatattcga aaagagatgc aataaattaa
     7561 ggagaggtta taatgaacaa agtaaatata aaagatagtc aaaattttat tacttcaaaa
     7621 tatcacatag aaaaaataat gaattgcata agtttagatg aaaaagataa catctttgaa
     7681 ataggtgcag ggaaaggtca ttttactgct ggattggtaa agagatgtaa ttttgtaacg
     7741 gcgatagaaa ttgattctaa attatgtgag gtaactcgta ataagctctt aaattatcct
     7801 aactatcaaa tagtaaatga tgatatactg aaatttacat ttcctagcca caatccatat
     7861 aaaatatttg gcaacatacc ttacaacata agcacaaata taattcgaaa aattgttttt
     7921 gaaagttcag ccacaataag ttatttaata gtggaatatg gttttgctaa aatgttatta
     7981 gatacaaaca gatcactagc attgctgtta atggcagagg tagatatttc tatattagca
     8041 aaaattccta ggtattattt ccatccaaaa cctaaagtgg atagcacatt aattgtatta
     8101 aaaagaaagc cagcaaaaat ggcatttaaa gagagaaaaa aatatgaaac ttttgtaatg
     8161 aaatgggtta acaaagagta cgaaaaactg tttacaaaaa atcaatttaa taaagcttta
     8221 aaacatgcga gaatatatga tataaacaat attagtttcg aacaatttgt atcgctattt
     8281 aatagttata aaatatttaa cggctaaaaa caataggcca catgcaactg taaatgttta
     8341 cgcgggtacc gacaccgcgg tggaggggaa ttcccatgtc agccgttaag tgttcctgtg
     8401 tcactcaaaa ttgctttgag aggctctaag ggcttctcag tgcgttacat ccctggcttg
     8461 ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt atatattctt ttttttctta
     8521 taaaacttaa aaccttagag gctatttaag ttgctgattt atattaattt tattgttcaa
     8581 acatgagagc ttagtacgtg aaacatgaga gcttagtacg ttagccatga gagcttagta
     8641 cgttagccat gagggtttag ttcgttaaac atgagagctt agtacgttaa acatgagagc
     8701 ttagtacgtg aaacatgaga gcttagtacg tactatcaac aggttgaact gctgatcttc
     8761 agatcctcta cgccggacgc atcgtggccg gatcaattcc gttttccgct gcataaccct
     8821 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
     8881 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
     8941 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
     9001 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccggctacc gccggcgtaa
     9061 cagatgaggg caagcggatg gctgatgaaa ccaagccaac caggaagggc agcccaccta
     9121 tcacggaatt gatccccctc gaattggaat tctagcctgg aatagggaag caccacatca
     9181 cc
//
