LOCUS       sequence-496832-        4479 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  Mammalian expression of Clontech rtTA driven by human beta-actin 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Faedo A, Laporta A, Segnali A, Galimberti M, Besusso D, Cesana E, 
            Belloli S, Moresco RM, Tropiano M, Fuca E, Wild S, Bosio A, Vercelli
            AE, Biella G, Cattaneo E
  TITLE     Differentiation of human telencephalic progenitor cells into MSNs by
            inducible expression of Gsx2 and Ebf1.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):E1234-E1242. doi: 
            10.1073/pnas.1611473114. Epub 2017 Jan 30.
  PUBMED    28137879
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1611473114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-02-14- 14"; volume: "114"; issue: "7"; pages: 
            "E1234-E1242"
COMMENT     Sequence Label: pCAG-TetON-3G
FEATURES             Location/Qualifiers
     source          1..4479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(9..755)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(786..805)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          813..1829
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(867..889)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(1830..2106)
                     /label=chicken beta-actin promoter
     enhancer        2108..2487
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2518..2622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2623..2691,2692..3483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2841..2860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3654..4242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4143..4162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4396..4413
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttacttagtt acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc
       61 atatcaaggt caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca
      121 agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt
      181 agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct
      241 tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact
      301 tgatgctcct gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg
      361 tacagtgcgt tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg
      421 gtttcgtact gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt
      481 gagaggagag cacagcggta tgacttggcg ttgttccgca gaaagtcttg ccatgactcg
      541 ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg
      601 agcagggccc gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt
      661 tgagcgagtt tccttgtcgt caggccttcg ataccgactc cattgagtaa ttccagagca
      721 gagtttatga ctttgctctt gtccagtcta gacatggtga attctttgcc aaaatgatga
      781 gacagcacaa taaccagcac gttgcccagg agctgtagga aaaagaagaa ggcatgaaca
      841 tggttagcag aggctctaga gccgccggtc acacgccaga agccgaaccc cgccctgccc
      901 cgtccccccc gaaggcagcc gtccccccgc ggacagcccc gaggctggag agggagaagg
      961 ggacggcggc gcggcgacgc acgaaggccc tccccgccca tttccttcct gccggcgccg
     1021 caccgcttcg ccccgcgccc gctagagggg gtgcggcggc gcctcccaga tttcggctcc
     1081 gcacagattt gggacaaagg aagtccctgc gccctctcgc acgattacca taaaaggcaa
     1141 tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc gggggccgcc gcgcccctcc
     1201 cccgagccct ccccggcccg aggcggcccc gccccgcccg gcacccccac ctgccgccac
     1261 cccccgcccg gcacggcgag ccccgcgcca cgccccgtac ggagccccgc acccgaagcc
     1321 gggccgtgct cagcaactcg gggagggggg tgcagggggg gttgcagccc gaccgacgcg
     1381 cccacacccc ctgctcaccc ccccacgcac acaccccgca cgcagccttt gttcccctcg
     1441 cagccccccc cgcaccgcgg ggcaccgccc ccggccgcgc tcccctcgcg cacactgcgg
     1501 agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag cgcggagccg
     1561 cacgcggcgc tccccacgca cacacacacg cacgcacccc ccgagccgct ccccccgcac
     1621 aaagggccct cccggagccc ctcaaggctt tcacgcagcc acagaaaaga aacaagccgt
     1681 cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc gctcacctgt
     1741 gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcggc ggagcggggc
     1801 acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg cgcttcgctt tttatagggc
     1861 cgccgccgcc gccgcctcgc cataaaagga aactttcgga gcgcgccgct ctgattggct
     1921 gccgccgcac ctctccgcct cgccccgccc cgcccctcgc cccgccccgc cccgcctggc
     1981 gcgcgccccc cccccccccc gcccccatcg ctgcacaaaa taattaaaaa ataaataaat
     2041 acaaaattgg gggtggggag gggggggaga tggggagagt gaagcagaac gtggggctca
     2101 cctcgaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     2161 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     2221 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     2281 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     2341 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     2401 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     2461 attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg aaatgtgcgc
     2521 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2581 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2641 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2701 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2761 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2821 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2881 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2941 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3001 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3061 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3121 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3181 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3241 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3301 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3361 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3421 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3481 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3541 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3601 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3661 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3721 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3781 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3841 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3901 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3961 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4021 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4081 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4141 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4201 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4261 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4321 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4381 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4441 ccgcctctcc ccgcgcgttg gccgattcat taatgcagc
//
