LOCUS       sequence-497384-        9430 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  Inducible promoter Pxyl expressing msfGFP in haloarcheon Haloferax 
            volcanii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Rados T, Andre K, Cerletti M, Bisson A
  TITLE     A sweet new set of inducible and constitutive promoters in Haloferax
            volcanii.
  JOURNAL   Front Microbiol. 2023 Aug 10;14:1204876. doi: 
            10.3389/fmicb.2023.1204876. eCollection 2023.
  PUBMED    37637112
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2023-08-10"; volume: "14:1204876. doi"; pages: "
            10.3389/fmicb.2023.1204876. eCollection 2023"
COMMENT     Sequence Label: pAL750 :: msfGFP
FEATURES             Location/Qualifiers
     source          1..9430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(775..1363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(855..874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1534..2325,2326..2394))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2157..2176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2395..2499)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3875..4233)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3915..3936)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4127..4146
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4360..4382
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4374..4391
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4375..4391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4401..4420
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4401..4419
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5937..5953)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5937..5953)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6012..6032)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6012..6030)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6051..6067)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6051..6067)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6064..6086)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6075..6091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6099..6105,6106..6123,6124..6129))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6144..6165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6282..6299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgtcaagcac caccgcaatt gtctcatcgt atgaccgtcg gccttcccgc gccttgagaa
       61 cccggaggtc gcgccgcaca tccttcgaca ccggaatccg cgtgtccgtg gcagacatat
      121 atcccgcttt agggcacaac gtataaagca ttacggacat gaagatgtga ttggtaaagc
      181 atgagtcttg acgaaagggc aagagggatt ttgtcaccgg ccgaccgccg gtatctccaa
      241 aaaccgaagg agtatagcaa acaggcagga tacgaacgcc ggcgagcgat agtcgagcgg
      301 ctccatgaag ccctacatga tatgccgctc ctggtttcgg aactcgacga ggaccttaga
      361 acggaagcct tcgaggacgg cgatttagac gggaaagagc atacgctaaa cgtcctgtcg
      421 tggtcgttcg ctctcctata tctcgggatc accgacaccg tcgaaccgtc cgatttggca
      481 aaggacgctt tcgaggggat tgccgcgagc ggtgttagac aggcatacct ccagcgcggg
      541 tattcggtcg aggaggtcga ggtcgaggtt aatgtaaaac ggggcgagcc gctcgacgag
      601 ttacgggagc gcgagccgga cgaactcagt taccccgagg tgaaccaact actcgaatcg
      661 ggcgaactct cccacctaac cggcgaggaa caaatggctc gaatcaaaga agctcatgtg
      721 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      781 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      841 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      901 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      961 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1021 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1081 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1141 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1201 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1261 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1321 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1381 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1441 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1501 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1561 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1621 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1681 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1741 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1801 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1861 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1921 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1981 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2041 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2101 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2161 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2221 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2281 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2341 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2401 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2461 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2521 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2581 tcatttttta accaataggc cgaaatcggc aaaatccctt aagcttactc atcggattcc
     2641 tccgcgccgc ggtcagccgc ggcgggctgt ttgcgctcgt acgtgacgaa cgaaaagccg
     2701 tcgcggtcgt cgcggtcggt ctcgacccac ttcccgcggt cccactcggg gaaccgcgtg
     2761 tcgccttcgt gtgccgcgtc gagttcggtg agcaccagcc ccgcggcgcg gccgaggaac
     2821 tgctcgtaga cggtcgcgcc gccgacgacg tagacggtct cgacgcccat ctcgtcggcc
     2881 gcgtcctcgg cggcggcgac cgccgactcg accgactcga ccgcttcagc gccctcgggg
     2941 aggtcgaggt cgcgggtcgt cagcacgacg ctgtgacgct ccggaagcgg gccgccgagt
     3001 tggcgggcga tgctctcgta ggtcttgcga cccatcacga ccgggtggcc ggtcgtcgtc
     3061 cgcttgaact gtttcaggtc ctcggggagg tgccacggca tgtcgccgtc gcggccgatg
     3121 acgcggttgt cggcgacggc ggcgacgagg acgaaccgga ccgcggcgtc gccctcggtt
     3181 tcggacccag aaagctcctc gccgctcatc tagattagcc gtcggcgtcg gccagcaggt
     3241 cctcggcggt gagaagcgac tccagtgcga tgtcgtggtc ggccagcagc tccgccgcgc
     3301 cctcctgtcg gtccacgacg acgacgacgc ggtcgacgac tgcgccggcc tcgcggagcg
     3361 cctccacggc gtcgacggcg ctctggccgg tcgtcgcgat gtcttcgagg acgacgactt
     3421 cctcgccctc gtcgagcgcg ccctcgatgc gctttgccgt cccgtactcc tttgccttct
     3481 tgcgggcgat gacgtacggc ttgttcgtct cgaccgcggt ggcggccacg agcgggaccg
     3541 cgccgagcgc gacgccggcc agtttgtcgt cgtccgcgac gcgctctgcg aacgcttcgg
     3601 cgatgagctt cagacaccgg gggtcggtct cgaagaggta cttgtccacg tagtagctcg
     3661 acgtgccgcc gtgggagagc tcgaactcgc cgaactggac cgcgtcggcg gctcggagcg
     3721 cctcgatgag tgctgcgttc gccatggctg cagagtgcgg cttccgaacg catagtagtt
     3781 gctgttcggg ccagcgtact gccacgaaag cccgggtgtg accgccatca cgacgctgcc
     3841 cgcggcgtca acgaggggtt ttatccacgg gatctaaatc aaaagaatag accgagatag
     3901 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3961 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4021 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4081 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4141 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4201 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     4261 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     4321 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     4381 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt acccccgtct
     4441 ttcgtctccc gtctcccgtc tctcgtctcc cgtttcgcca tcgacgcgct ctcgtgagcg
     4501 cgcgctagcc cctcccgacg ggagtttcga tgtcgcttcc gacaggtttg ccgccggcag
     4561 acgccggaga ctgctggaca atcgttctgt aatagggaac gaagggcgag gccgagtgac
     4621 tcgtcccccg cgttcgctct tgctccggac tctcgggaac cccgcccccc ttctcggcgt
     4681 cggccacccg aattacaagc gtatgtgtgt aatctaaaac cggcgcaccg cacgtcgcgt
     4741 gggctgttcc cggactctct cgttcgtgcg tgctgtgatt gcattctgtg atagaaaacg
     4801 gcagtgtctg gcgaactctc ggcccactcg ttcctgatta tcggccgaga cgagccgtaa
     4861 taatcgactt gtctctctcg gtggtcgttt tcgacgacac cggtgtgggt cgtcagtccg
     4921 cgctcgtggg ctccgacacg gccgtctcgt cgccgcgttg ccgattcgac ccgccgactc
     4981 ggcgtgttct gttactggga attcgtgtgt ctctctacgt cacacgcgtc gggctcgtgt
     5041 cgcctcttct gcctgtcatc tcgcgctcgt cggcgctcgt cgtcaacgga cgagcgaact
     5101 acacccggcc gggagttcga gaccgaaccc gccgacgtct cggggttttc tgcattgccc
     5161 cgcgccgccc ataccgggac gtacttttat tacaatcgct ggtaatgagg atactgcaat
     5221 gcgaaaaggg gaagaattgt ttacaggcgt agtaccgata ttagttgagc tggatggaga
     5281 tgttaacgga cacaagtttt ccgtacgcgg agagggagaa ggcgatgcca ccaatggcaa
     5341 actcaccctt aaatttattt gtacgacagg caagctacca gtgccgtggc ctacattggt
     5401 cactacactc acgtatggtg tgcaatgctt tgcaagatat cccgaccaca tgaagcaaca
     5461 tgactttttc aaatcagcca tgcctgaagg atatgtccaa gaaaggacta ttagctttaa
     5521 ggacgacggc acctacaaaa cacgcgctga agtcaaattc gaaggtgata cgttagttaa
     5581 tcgtatcgaa ctgaaaggta tagactttaa agaagatgga aatatcctgg ggcataagtt
     5641 ggagtacaat ttcaacagcc ataacgtata catcacagct gacaagcaga aaaatggcat
     5701 taaagcaaat ttcaaaatca gacataacgt tgaagatggg tcagttcagc ttgcggatca
     5761 ttatcaacag aacacaccga ttggcgatgg tccggtgtta ctacctgata accattatct
     5821 gtcgactcag agtaaactgt ctaaagatcc aaatgaaaaa cgggaccaca tggtcctttt
     5881 agagtttgtg acggcagctg ggattacgca tggaatggat gaactttaca aatgaggatc
     5941 cactagttct agagcggccg cacctctgga ccatcgcatt tttcggcgcg gtggagctcc
     6001 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     6061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6121 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6181 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6241 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6361 tccacagaat caggggataa cgcaggaaag aacatgcatg gggggttgtt gtccgaccgc
     6421 gattcatagg ttcagagttc gtcgacccta ctctaatcat gaacgtactg tctcgagaga
     6481 taccacgaga ttacgagata cccgaataca acgcatatca cgaacatatt accaaaggtt
     6541 agttcaatca cgaacctgcc tccgtgaggg gttgctgtgc aatctccggc ccaatagaca
     6601 gcgtctgaac cgtctcgtct cgttcttcgg ttagactcga cagtaaggga cgtacccagt
     6661 ctacccagtc gtacatcgta tggagacggt cgatgtccgg tagcccgtta ggtccgcatt
     6721 gtacccatat ctcgattacc tcctcggtca actcatcaag cgcttctagc gtctcaaaga
     6781 ggacgtctcg agtgaacgac tcggtcagca tgaacgatgg aagaatccgc ttgtggcgtt
     6841 cttgctcgtc tgtgaacgaa aggttcagtc ggcactgacc ctcggtctcg tcgactaacc
     6901 ccattgctac aagtgtcggt aagtggttcc gaaccgactg cgtcgagatt cccgctttct
     6961 cggctaactc tgtcttgcta aggctcagat tcgtcgcgag caacgtacta atcatcgccc
     7021 gaaccgtggg ggttgcgtcc tctaagagac gcttcgacgg gagactcgcg agtgaaagcc
     7081 gtacttcgct actccgaatc tctctcggtg tcgcttcttg agacaaccct cgcccgattg
     7141 cgtgggctac gtcgtacggt gtccgtgcaa acccagagaa tagccgtatc gcgtctctcg
     7201 tcgcgtcgag acctttcgcc cgacacattg cccgtgttac tcgtgcaacc gtactcggac
     7261 catgctcgac gaccacaggg actttgagcg caatctccgg cgcgtcctcg tgaatctccc
     7321 cagggttcga cagtcggtct tctattacgc cggcgacgtg gtccgcccga ttcccgaagt
     7381 caccaaccac cgagatcgac gcaatcaggt tcgcatacgg attgctcgcg tcgacgacag
     7441 ggtcgaacga ctgacgtcgc ttctcgtctc ggtcttcaaa caacagtcgc cggactacgt
     7501 ggttcccgta cgtcgccgca atcggcgcgt tcttcgcaag cgtcgttgct aacaactcgg
     7561 cattgtcctc cccgtatcgc tccgggtgtc ggttgtagtc gggtaagcga gcctcccaaa
     7621 cgacgtcaat atcgagtaaa tcgaggacgt gaaacgcggt tcccgcgaga cccatcgccg
     7681 ttttcgtgat tcgactcagg tactcggacg tctcacactc gtagtcctca tgtgcaagtg
     7741 accgcgtcat gtcgagtagc tcgtctctcg cgtcctcgag gacgtcgacg tactcacccg
     7801 ccgagtcgac agagtcagcc aaccacccga ggtttcgaga cccacgaacg tacgtcccat
     7861 cttcgattag tccatcaagt tccgcgagac ggtcctcggt aaggacctgg tcgaacgtct
     7921 taggatcgag taacgctcgt gcaatcatca ccacatgaga catggcagag tcgtactctg
     7981 cagagacgac gagcgtattc tcgtcctcaa ggtagcccca cccggcttct ccccggtcag
     8041 atttgggttc gacagggtag tttgtcacac taacgccttt cttcggcggt gtcttcgccg
     8101 ctgagaccgt ctctgcgtac gaaagatagt ccacaccgtg ataccgaggt aagcgaaccc
     8161 gtccgtccgg cgtcgtcgac gcctcacctt cggcggtcgg tcggtggtcg tcctccctcc
     8221 ctccccgagg gcggtccgtg attacacggg tattgtggtt tggttttggg gtgttcgtaa
     8281 cgtagtcctg aagggccttt tgtcgctctt tctctttggc taattcttcg aggtacaacg
     8341 ctcctttgtc gagaatctcg attcgtaggt ttccgcgagc gccgtacttc tcacaaagac
     8401 catactcgat tagcttctcg ttcaatcgcc gcgagaccgt ggtcctatcg tactgactga
     8461 acgccgattg tagggcgctg taggccatgt gtccgctttc agtctcacct accgtccgaa
     8521 gtatctcgac agcaagtccg tccgtcccaa ccacgtcgac gagcgtcgta atggagtaat
     8581 cattacgggt gtcgttgcac ggattcgtaa cactcgatgg gagaatatcg ccgtagtagt
     8641 tgtgcaagta cactcggtcg atctctgacc caccaaggac cacgtcgacg ctctcagaca
     8701 gcgttagaac gtactcgata atctcgtcgg cctggtcgcg ccgcgtctct ctgaaagccg
     8761 cattgagacc gattgctatc gtctgttgag ggttgctata gcccaataga cgagtgaacg
     8821 cgagagcgtc ctctaaggcg ctctgtggct gtcccatctc atcatgtccc aaccgtcgcg
     8881 cataccgaat caagtcccac agagcccgtc tctcgccggc gacagctcgt cgtgcaagtt
     8941 tcacgactga cccatcgacg tcaaacccac aactcgacag tcggtgttca aactcgagta
     9001 tgtcgacacc gtccgccgct gtttgtagtg cctcgagaag cgcaaattga ctctctgtct
     9061 cgtcgaagat tactcgatga acccgaaggg cggtccagtc tgtaagcgag acgtccgcct
     9121 cgaggtcagt cgcaaagcga aggcttcgct tctcagtcgg ttcgtgtgcc gtcgtatcgt
     9181 ccgtatcgta catttagtgt cgcttttgtg cgaacactaa cgccgggtga aaatccgata
     9241 atcccataag ccccaaccac attggttgag ttaggacaac gggccacccg cccgccgtgt
     9301 ccgcgtaagc atccttcgac gccgatagga gcgaagtcct ggcagactcg tgtgctcccg
     9361 gcgtcgtttg attcaactag ttcgctagtc acgactatag tttattaatc caccttctca
     9421 gagagatatg
//
