LOCUS       sequence-497470-        9591 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N)).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, 
            Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters 
            Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek 
            M, Schulman BA, Bode JW, Corn JE
  TITLE     C-terminal amides mark proteins for degradation via SCF-FBXO31.
  JOURNAL   Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w.
  PUBMED    39880951
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2025-02"; volume: "638"; issue: "8050"; pages: 
            "519-527"
COMMENT     Sequence Label: pJC2740 - pHR-EF1A-HA-FBXO31(D334N)-IRES-mTagBFP
FEATURES             Location/Qualifiers
     source          1..9591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2372..2570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2525..2545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2588..2768
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2815..2940
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3437..3670
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3855..3899
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4197..4314
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4379..4426
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        4503..4714
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4715..5653
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5610..5630
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5677..5703
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7360..7413
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7426..8124
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(8144..8177)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8233..8821
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8286..8306)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8704..8715)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8824..8840)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8825..8841)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9350..9530
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     2581 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2641 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2701 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2761 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2821 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2881 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2941 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3001 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3061 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3121 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3181 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3241 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     3301 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     3361 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     3421 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     3481 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     3541 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     3601 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     3661 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     3721 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     3781 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     3841 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     3901 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     3961 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     4021 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     4081 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4141 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     4201 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4261 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     4321 tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgccctg caggtctaga
     4381 agttcctatt ccgaagttcc tattctctag aaagtatagg aacttctcga gtactaggat
     4441 ccattaggcg gccgccctgc agggtggata accgtattac cgccatgcat gtgcccgtca
     4501 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4561 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4621 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4681 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4741 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4801 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4861 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4921 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4981 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     5041 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5101 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5161 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5221 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5281 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5341 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5401 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgcgcttttg
     5461 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5521 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5581 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5641 tcttccattt caggtgtcgt gagctagccc accatgtacc catacgatgt tccagattac
     5701 gctggtggat ctggaggttc tggtggaagc gctatggcgg tgtgtgctcg cctttgcggc
     5761 gtgggcccgt cgcgcggatg tcggcgccgc cagcagcgcc ggggcccggc cgagacggcg
     5821 gcggccgaca gcgagccgga cacagacccc gaggaggagc gcatcgaggc tagcgccggg
     5881 gtcgggggcg gcttgtgcgc gggcccctcg ccgccgcccc cgcgctgctc gctgctggag
     5941 ctgccgcccg agctgctggt ggagatcttc gcgtcgctgc cgggcacgga cctacccagc
     6001 ttggcccagg tctgcacgaa gttccggcgc atcctccaca ccgacaccat ctggaggagg
     6061 cgttgccgtg aggagtatgg tgtttgcgaa aacttgcgga agctggagat cacaggcgtg
     6121 tcttgtcggg acgtctatgc gaagctgctt caccgatata gacacatttt gggattgtgg
     6181 cagccagata tcgggccata cggaggactg ctgaacgtgg tggtggacgg cctgttcatc
     6241 atcgggtgga tgtacctgcc tccccatgac ccccacgtcg atgaccctat gagattcaag
     6301 cctctgttca ggatccacct gatggagagg aaggctgcca cagtggagtg catgtacggc
     6361 cacaaagggc cccaccacgg ccacatccag attgtgaaga aggatgagtt ctccaccaag
     6421 tgcaaccaga cggaccacca caggatgtcc ggcgggaggc aggaggagtt tcggacgtgg
     6481 ctgagggagg aatgggggcg cacgctggag gacatcttcc acgagcacat gcaggagctc
     6541 atcctgatga agttcatcta caccagtcag tacgacaact gcctgaccta ccgccgcatc
     6601 tacctgccgc ccagccgccc cgacgacctc atcaagcctg gcctcttcaa aggtacctat
     6661 ggcagccacg gcctggagat tgtgatgctc agcttccacg gccggcgtgc caggggcacc
     6721 aagatcacgg gcaaccccaa catccccgct gggcagcaga cagtggagat cgacctgagg
     6781 catcggatcc agctgcccga cctcgagaac cagcgcaact tcaatgagct ctcccgcatc
     6841 gtcctggagg tgcgcgagag ggtgcgccag gagcagcagg aaggcgggca cgaggcgggc
     6901 gagggtcgtg gccggcaggg cccccgggag tcccagccaa gccctgccca gcccagggca
     6961 gaggcgccca gcaagggccc agatgggaca cctggtgagg atggtggcga gcctggggat
     7021 gccgtagctg cggccgagca gcctgcccag tgtgggcagg ggcagccgtt cgtgctgccc
     7081 gtgggcgtga gctccaggaa tgaggactac ccccgaacct gcaggatgtg tttttatggc
     7141 acaggcctca tcgcgggcca cggcttcacc agccctgaac gcacccccgg ggtcttcatc
     7201 ctcttcgatg aggaccgctt cgggttcgtc tggctggagc tgaaatcctt cagcctgtac
     7261 agccgggtcc aggccacctt ccggaacgca gatgcgccgt ccccacaggc cttcgatgag
     7321 atgctcaaga acattcagtc cctcacctcc ggatcgggag agggcagagg aagtctgcta
     7381 acatgcggtg acgtcgagga gaatcctggc ccaccggtcg ccaccagcga gctgattaag
     7441 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
     7501 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     7561 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     7621 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     7681 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     7741 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     7801 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg
     7861 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     7921 gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac
     7981 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     8041 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
     8101 aaactggggc acaagcttaa ttaagaattc gtcgagggac ctaataactt cgtatagcat
     8161 acattatacg aagttataca tgtttaaggg ttccggttcc actaggtaca attcgatatc
     8221 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8281 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8341 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8401 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8461 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8521 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8581 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8641 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8701 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8761 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     8821 catcgatacc gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa
     8881 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     8941 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     9001 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     9061 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     9121 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     9181 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     9241 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     9301 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     9361 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9421 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9481 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc
     9541 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt t
//
