LOCUS       sequence-497528-        2320 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH41402
FEATURES             Location/Qualifiers
     source          1..2320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..817
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      988..1576
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1477..1496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1730..1747
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1839..1894
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     complement(1934..1952)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2020..2124
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
       61 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      121 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt
      181 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      241 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      301 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      361 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      421 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      481 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      541 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
      601 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      661 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      721 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta
      781 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata
      841 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      901 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      961 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1021 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1081 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1141 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1201 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1261 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1321 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1381 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1441 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1501 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1561 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1621 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1681 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1741 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1801 tcattaatca ctctgtggtc tcatactgta tttttacaac aattaccaac aacaacaaac
     1861 aacaaacaac attacaatta ctatttacaa ttacaatgtg agaccacgaa gtggctcttc
     1921 agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1981 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2041 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2101 caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     2161 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg
     2221 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2281 tgatgttatg gagcagcaac gatgttacgc agcagggcag
//
