LOCUS       sequence-497530-        2946 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL80004
FEATURES             Location/Qualifiers
     source          1..2946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     121..140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     374..391
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1204..1222)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1290..1394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1616..2407
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2578..2946,1..220)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
       61 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      121 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      181 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      241 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      301 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      361 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      421 gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcaaat gagcgagctt
      481 atcaaagaaa acatgcacat gaagctctac atggaaggta ctgtgaacaa ccaccacttc
      541 aagtgcacat ctgagggtga gggaaagcct tacgagggaa ctcagaccat gaagatcaag
      601 gttgtcgagg gtggacctct ccctttcgct ttcgatatcc ttgctacctc cttcatgtac
      661 ggaagcaagg ccttcatcaa ccacactcag ggtatccctg acttcttcaa gcagtctttc
      721 ccagagggat tcacctggga gagaatcact acttacgagg atggtggtgt tctcaccgct
      781 actcaggata cctctttcca gaacggatgc atcatctaca acgtcaagat caacggtgtg
      841 aacttcccta gcaacggacc tgtgatgcag aaaaagacta gaggatggga ggccaacacc
      901 gagatgcttt accctgctga tggtggactt agaggacact ctcagatggc tcttaagctc
      961 gttggtggtg gatacctcca ctgctctttc aagaccacct accgttctaa gaagcctgcc
     1021 aagaacctca agatgcctgg attccacttc gtggatcaca gactcgagag gatcaaagag
     1081 gccgacaaag agacttacgt tgagcagcat gagatggccg ttgctaagta ctgcgatctc
     1141 ccatctaagc tcggacacag ataggctttg agaccacgaa gtggctcttc agtggacgaa
     1201 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     1261 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1381 gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1441 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg gggtacagtc
     1501 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     1561 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     1621 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     1681 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     1741 gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     1801 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     1861 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     1921 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     1981 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     2041 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     2101 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     2161 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     2221 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     2281 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     2341 cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg
     2401 caaataactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2461 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2521 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2581 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2641 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2701 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2761 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2821 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2881 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2941 caccga
//
