LOCUS       sequence-497536-        2323 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH44199
FEATURES             Location/Qualifiers
     source          1..2323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(102..893)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1115..1219)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1287..1305
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1495..1512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1666..2254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1746..1765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
       61 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttatttgcc gactaccttg
      121 gtgatctcgc cttttacgta atggacaaat tcttccaact gatctgcgcg cgaggccaag
      181 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
      241 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
      301 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
      361 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
      421 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
      481 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
      541 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
      601 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
      661 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
      721 agccttacgg tcaccgtaac cagcaaatca atatcgctgt gtggcttcag gccgccatcc
      781 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
      841 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct catgatgttt
      901 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
      961 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     1021 aaaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     1081 cggtcaaggt tctggaccag ttgcgtgagc gcatactctt cctttttcaa tattattgaa
     1141 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1201 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1261 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtccactga
     1321 agagccactt cgtggtctca cattttgtta gacttgcttt tccaagcggg tgtgtaacaa
     1381 ttgggcgtgg tgggtttggt tgtgttggtg gtgtagtatg agaccacaga gtgattaatg
     1441 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1501 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1561 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1621 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1681 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1741 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1801 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1861 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1921 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1981 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2041 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2101 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2161 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2221 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2281 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttg
//
