LOCUS       sequence-497719-        9469 bp    DNA     circular SYN 16-JAN-2026
DEFINITION  Express GSDMD WT in mammalian cells with lentiviurs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Du G, Healy LB, David L, Walker C, El-Baba TJ, Lutomski CA, Goh B, 
            Gu B, Pi X, Devant P, Fontana P, Dong Y, Ma X, Miao R, 
            Balasubramanian A, Puthenveetil R, Banerjee A, Luo HR, Kagan JC, Oh 
            SF, Robinson CV, Lieberman J, Wu H
  TITLE     ROS-dependent S-palmitoylation activates cleaved and intact 
            gasdermin D.
  JOURNAL   Nature. 2024 Jun;630(8016):437-446. doi: 10.1038/s41586-024-07373-5.
            Epub 2024 Apr 10.
  PUBMED    38599239
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07373-5"; journalName: "Nature"; date: 
            "2024-06"; volume: "630"; issue: "8016"; pages: "437-446"
COMMENT     Sequence Label: plenti-puro-GSDMD WT
FEATURES             Location/Qualifiers
     source          1..9469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2104..2603
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2139..2162)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2536..2555
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2624..3223
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2624..2643)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3120..3140
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3237..3825
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3290..3310)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3708..3719)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4032..4665
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4794..4810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4794..4810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4807..4829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4818..4834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4842..4848,4849..4866,4867..4872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4887..4908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5025..5042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5196..5784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5276..5295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5955..6746,6747..6815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6578..6597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6816..6920)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6968..7102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7005..7024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7059..7078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7270..7903
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7950..8075
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8572..8805
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8990..9034
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9297..9412
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tcgctagcgc
      601 taccggactc agatctcgag atggggtcgg cctttgagcg ggtagtccgg agagtggtcc
      661 aggagctgga ccatggtggg gagttcatcc ctgtgaccag cctgcagagc tccactggct
      721 tccagcccta ctgcctggtg gttaggaagc cctcaagctc atggttctgg aaaccccgtt
      781 ataagtgtgt caacctgtct atcaaggaca tcctggagcc ggatgccgcg gaaccagacg
      841 tgcagcgtgg caggagcttc cacttctacg atgccatgga tgggcagata cagggcagcg
      901 tggagctggc agccccagga caggcaaaga tcgcaggcgg ggccgcggtg tctgacagct
      961 ccagcacctc aatgaatgtg tactcgctga gtgtggaccc taacacctgg cagactctgc
     1021 tccatgagag gcacctgcgg cagccagaac acaaagtcct gcagcagctg cgcagccgcg
     1081 gggacaacgt gtacgtggtg actgaggtgc tgcagacaca gaaggaggtg gaagtcacgc
     1141 gcacccacaa gcgggagggc tcgggccggt tttccctgcc cggagccacg tgcttgcagg
     1201 gtgagggcca gggccatctg agccagaaga agacggtcac catcccctca ggcagcaccc
     1261 tcgcattccg ggtggcccag ctggttattg actctgactt ggacgtcctt ctcttcccgg
     1321 ataagaagca gaggaccttc cagccacccg cgacaggcca caagcgttcc acgagcgaag
     1381 gcgcctggcc acagctgccc tctggcctct ccatgatgag gtgcctccac aacttcctga
     1441 cagatggggt ccctgcggag ggggcgttca ctgaagactt ccagggccta cgggcagagg
     1501 tggagaccat ctccaaggaa ctggagcttt tggacagaga gctgtgccag ctgctgctgg
     1561 agggcctgga gggggtgctg cgggaccagc tggccctgcg agccttggag gaggcgctgg
     1621 agcagggcca gagccttggg ccggtggagc ccctggacgg tccagcaggt gctgtcctgg
     1681 agtgcctggt gttgtcctcc ggaatgctgg tgccggaact cgctatccct gttgtctacc
     1741 tgctgggggc actgaccatg ctgagtgaaa cgcagcacaa gctgctggcg gaggcgctgg
     1801 agtcgcagac cctgttgggg ccgctcgagc tggtgggcag cctcttggag cagagtgccc
     1861 cgtggcagga gcgcagcacc atgtccctgc cccccgggct cctggggaac agctggggcg
     1921 aaggagcacc ggcctgggtc ttgctggacg agtgtggcct agagctgggg gaggacactc
     1981 cccacgtgtg ctgggagccg caggcccagg gccgcatgtg tgcactctac gcctccctgg
     2041 cactgctatc aggactgagc caggagcccc actagggatc ccgcgactct agataattct
     2101 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     2161 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     2221 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     2281 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     2341 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     2401 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     2461 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     2521 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     2581 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     2641 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     2701 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     2761 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     2821 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     2881 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     2941 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     3001 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     3061 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     3121 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     3181 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc
     3241 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3301 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3361 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3421 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3481 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3541 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3601 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     3661 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3721 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3781 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     3841 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     3901 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     3961 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4021 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     4081 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     4141 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     4201 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     4261 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     4321 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     4381 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     4441 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     4501 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4561 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4621 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     4681 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     4741 gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     4801 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4861 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4921 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     4981 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5041 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5101 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5161 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5221 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5281 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5341 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5401 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5461 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5521 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5581 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5641 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5701 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5761 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5821 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5881 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5941 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6001 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6061 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6121 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6181 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6241 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6301 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6361 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6421 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6481 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6541 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6601 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6661 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6721 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6781 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6841 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6901 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     6961 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7021 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7081 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     7141 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     7201 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     7261 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     7321 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     7381 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     7441 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     7501 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     7561 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     7621 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     7681 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7741 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7801 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7861 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     7921 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     7981 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8041 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8101 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8161 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8221 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8281 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8341 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8401 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8461 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8521 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8581 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8641 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8701 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8761 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8821 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8881 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8941 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9001 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9061 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9121 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9181 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     9241 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta
     9301 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9361 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9421 attacaggga cagcagagat ccagtttatc gataagcttg ggagttccg
//
